PHD1 antibody (N-Term)
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- Target See all PHD1 (EGLN2) Antibodies
- PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))
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Binding Specificity
- N-Term
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PHD1 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
- Specificity
- EGLN2 Antibody detects endogenous levels of total EGLN2.
- Predicted Reactivity
- Pig,Rabbit,Dog
- Purification
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- Immunogen
- A synthesized peptide derived from human EGLN2, corresponding to a region within N-terminal amino acids.
- Isotype
- IgG
- Top Product
- Discover our top product EGLN2 Primary Antibody
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anti-Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2) (AA 283-407) antibody
EGLN2 Reactivity: Human ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2) (AA 194-221) antibodyEGLN2 Reactivity: Human WB, FACS, IHC (p) Host: Rabbit Polyclonal RB22708 unconjugated
anti-Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2) (AA 58-107) antibodyEGLN2 Reactivity: Human, Cow, Guinea Pig WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2) (AA 150-250) antibodyEGLN2 Reactivity: Human WB, IP Host: Rabbit Polyclonal unconjugated
anti-Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2) (AA 1-407) antibodyEGLN2 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 2G3 unconjugated
anti-Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2) antibodyEGLN2 Reactivity: Human WB Host: Rabbit Monoclonal unconjugated
anti-Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2) (AA 281-315) antibodyEGLN2 Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit, Horse, Pig, Monkey, Bat WB Host: Rabbit Polyclonal unconjugated
anti-Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2) (C-Term) antibodyEGLN2 Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Monkey WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2) antibodyEGLN2 Reactivity: Human, Mouse, Rat WB, IP Host: Rabbit Polyclonal unconjugated
anti-Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2) (Middle Region) antibodyEGLN2 Reactivity: Human, Dog WB Host: Rabbit Polyclonal unconjugated
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- Application Notes
- WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20 °C. Stable for 12 months from date of receipt.
- Expiry Date
- 12 months
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- Target
- PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))
- Alternative Name
- EGLN2 (EGLN2 Products)
- Synonyms
- egln2 antibody, phd2 antibody, PHD1 antibody, 0610011A13Rik antibody, C85656 antibody, Hif-p4h-1 antibody, Ier4 antibody, Phd1 antibody, SM-20 antibody, HIF-PH1 antibody, HPH-1 antibody, HPH-3 antibody, PHD-1 antibody, EIT6 antibody, HIFPH1 antibody, egl-9 family hypoxia-inducible factor 1 antibody, egl-9 family hypoxia inducible factor 2 antibody, Egl nine homolog 2 antibody, egl-9 family hypoxia-inducible factor 2 L homeolog antibody, egl-9 family hypoxia-inducible factor 2 antibody, egln1 antibody, EGLN2 antibody, egln2 antibody, egln2.L antibody, Egln2 antibody
- Background
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Description: Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN2 is involved in regulating hypoxia tolerance and apoptosis in cardiac and skeletal muscle. Also regulates susceptibility to normoxic oxidative neuronal death. Links oxygen sensing to cell cycle and primary cilia formation by hydroxylating the critical centrosome component CEP192 which promotes its ubiquitination and subsequent proteasomal degradation. Hydroxylates IKBKB, mediating NF-kappaB activation in hypoxic conditions. Target proteins are preferentially recognized via a LXXLAP motif.
Gene: EGLN2
- Molecular Weight
- 44 kDa
- Gene ID
- 112398
- UniProt
- Q96KS0
- Pathways
- Intracellular Steroid Hormone Receptor Signaling Pathway, Cell RedoxHomeostasis
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