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ADAM17 antibody (AA 201-300)

Name: ADAM17 antibody (AA 201-300)
SKU: ABIN705671
Price: 384.62 USD
Availability: InStock

ADAM17 Reactivity: Human, Rat, Mouse WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin embedded human placenta labeled with Anti-ADAM17/CD156b Polyclonal Antibody, Unconjugated (ABIN705671) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

IHC

Mouse thymocytes probed with Rabbit Anti-ADAM17 Polyclonal Antibody, Unconjugated .

FACS

U937 cells probed with ADAM17 Polyclonal Antibody, Unconjugated at 1:100 for 30 minutes followed by incubation with a PE conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

FACS

Paraformaldehyde-fixed, paraffin embedded rat kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with ADAM17 Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.

IHC (p)

4 images

(1 reference)

Catalog No. ABIN705671

Datasheet as PDF

Target See all ADAM17 Antibodies

ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))
Binding Specificity
15

13

8

8

8

7

6

5

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
AA 201-300
Reactivity
109

53

51

4

4

4

1

1
Human, Rat, Mouse
Host
94

13

2

1

1
Rabbit
Compatible Secondaries
Clonality
86

25
Polyclonal
Conjugate
53

10

7

5

4

4

3

3

3

3

3

3

1

1

1

1

1

1

1

1

1

1
This ADAM17 antibody is un-conjugated
Application
81

47

39

14

14

13

12

10

10

6

5

3

3

2

2
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity

Human, Mouse, Rat

Predicted Reactivity

Dog,Cow,Pig,Rabbit

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human ADAM17

Isotype

IgG

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anti-ADAM Metallopeptidase Domain 17 (ADAM17) (AA 226-473) antibody
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anti-ADAM Metallopeptidase Domain 17 (ADAM17) (C-Term) antibody
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KO Validated ADAM17 Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-ADAM Metallopeptidase Domain 17 (ADAM17) (pThr735) antibody
ADAM17 Reactivity: Human, Rat, Mouse WB, ELISA, IHC, ICC, IF Host: Rabbit Polyclonal unconjugated

anti-ADAM Metallopeptidase Domain 17 (ADAM17) antibody
ADAM17 Reactivity: Human, Rat, Mouse WB, FACS, IF (cc) Host: Rabbit Monoclonal 9E7 unconjugated

anti-ADAM Metallopeptidase Domain 17 (ADAM17) (N-Term) antibody
ADAM17 Reactivity: Human WB, FACS Host: Rabbit Polyclonal unconjugated

anti-ADAM Metallopeptidase Domain 17 (ADAM17) (AA 215-315) antibody
ADAM17 Reactivity: Human WB, ELISA, IHC, IHC (p), PLA Host: Mouse Monoclonal 1F6 unconjugated

anti-ADAM Metallopeptidase Domain 17 (ADAM17) (AA 215-315) antibody
ADAM17 Reactivity: Human WB, IHC (p), EIA Host: Mouse Monoclonal 1F6 unconjugated

Application Notes

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Li, Xie, He, Wang, Duan, Yang, Wang: "Identification of ADAM10 and ADAM17 with potential roles in the spermatogenesis of the Chinese mitten crab, Eriocheir sinensis." in: Gene, Vol. 562, Issue 1, pp. 117-27, (2015) (PubMed).
Target

ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))

Alternative Name

ADAM17 (ADAM17 Products)

Synonyms

ADAM18 antibody, CD156B antibody, CSVP antibody, NISBD antibody, TACE antibody, CD156b antibody, Tace antibody, adam17 antibody, si:dkey-81b15.2 antibody, adam18 antibody, cd156b antibody, csvp antibody, tace antibody, fc20b01 antibody, wu:fc20b01 antibody, zgc:63886 antibody, ADAM metallopeptidase domain 17 antibody, a disintegrin and metallopeptidase domain 17 antibody, ADAM metallopeptidase domain 17b antibody, ADAM metallopeptidase domain 17 L homeolog antibody, ADAM metallopeptidase domain 17a antibody, ADAM17 antibody, Adam17 antibody, adam17b antibody, adam17.L antibody, adam17a antibody

Background

Synonyms: CSVP, TACE, NISBD, ADAM18, CD156B, NISBD1, Disintegrin and metalloproteinase domain-containing protein 17, ADAM 17, Snake venom-like protease, TNF-alpha convertase, TNF-alpha-converting enzyme, ADAM17
Background: Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). Plays a role in the proteolytic processing of ACE2.

Gene ID

6868

UniProt

P78536

Pathways

Notch Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus