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CBX5 antibody (Internal Region)

Verified CBX5 Reactivity: Human WB, ELISA, IF, FACS Host: Goat Polyclonal unconjugated
Catalog No. ABIN185491
  • Target See all CBX5 Antibodies
    CBX5 (Chromobox Homolog 5 (CBX5))
    Binding Specificity
    • 15
    • 15
    • 12
    • 10
    • 10
    • 10
    • 7
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 113
    • 49
    • 28
    • 15
    • 8
    • 7
    • 7
    • 4
    • 4
    • 4
    • 3
    • 1
    • 1
    Human
    Host
    • 120
    • 18
    • 1
    • 1
    Goat
    Clonality
    • 110
    • 29
    • 1
    Polyclonal
    Conjugate
    • 67
    • 10
    • 8
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    This CBX5 antibody is un-conjugated
    Application
    • 92
    • 55
    • 28
    • 28
    • 26
    • 22
    • 17
    • 10
    • 10
    • 7
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
    Purpose
    CBX5 / HP1-Alpha
    Sequence
    NKRKSNFSNS ADDIK
    Specificity
    Variants (NP_036249.1, NP_001120793.1, NP_001120794.1) encode the same protein.
    Cross-Reactivity
    Dog, Human, Mouse
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-NKRKSNFSNSADDIK, from the internal region of the protein sequence according to NP_036249.1, NP_001120793.1, NP_001120794.1.
    Isotype
    IgG
    Top Product
    Discover our top product CBX5 Primary Antibody
  • Application Notes

    Western Blot: Approx 24-25 kDa band observed in lysates of cell lines A43N and K562 (calculated MW of 22.2 kDa according to Human NP_036249.1). Recommended concentration: 0.03-0.1 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:128000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    CBX5 (Chromobox Homolog 5 (CBX5))
    Alternative Name
    CBX5 (CBX5 Products)
    Synonyms
    HP1 antibody, HP1A antibody, id:ibd2026 antibody, wu:fc63e02 antibody, wu:fe47g12 antibody, wu:fi03h05 antibody, zgc:158601 antibody, CBX5 antibody, cbx5 antibody, MGC64354 antibody, 2610029O15Rik antibody, C75991 antibody, Hp1a antibody, Hp1alpha antibody, chromobox 5 antibody, chromobox homolog 5 (HP1 alpha homolog, Drosophila) antibody, chromobox 5 S homeolog antibody, CBX5 antibody, cbx5 antibody, Cbx5 antibody, cbx5.S antibody
    Background
    CBX5, HP1-ALPHA, chromobox homolog 5 (HP1 alpha homolog, Drosophila), HGNC:1555, HP1, HP1Hs-alpha , chromobox homolog 5 (Drosophila HP1 alpha), Heterochromatin protein-1, HP1A, antigen p25, heterochromatin protein 1 homolog alpha, heterochromatin protein
    Gene ID
    23468
    NCBI Accession
    NP_036249, NP_001120793, NP_001120794
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