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PLEKHH2 antibody (AA 1043-1092)

PLEKHH2 Reactivity: Human, Mouse, Rabbit, Cow, Dog, Guinea Pig, Horse, Bat, Monkey, Pig WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6741614
  • Target See all PLEKHH2 products
    PLEKHH2 (Pleckstrin Homology Domain Containing, Family H (With MyTH4 Domain) Member 2 (PLEKHH2))
    Binding Specificity
    • 8
    • 8
    • 6
    • 1
    AA 1043-1092
    Reactivity
    Human, Mouse, Rabbit, Cow, Dog, Guinea Pig, Horse, Bat, Monkey, Pig
    Host
    • 16
    • 1
    Rabbit
    Clonality
    • 17
    Polyclonal
    Conjugate
    • 7
    • 2
    • 2
    • 2
    • 2
    • 2
    This PLEKHH2 antibody is un-conjugated
    Application
    • 17
    • 14
    • 13
    • 13
    • 2
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Brand
    IHC-plus™
    Specificity
    Human PLEKHH2
    Predicted Reactivity
    Percent identity by BLAST analysis: Dog, Bovine, Rabbit, Horse, Guinea pig (100%) Rat (92%) Chicken (86%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic peptide located between aa1043-1092 of human PLEKHH2 (Q8IVE3, NP_742066). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Zebra finch (100%), Rat, Opossum (92%), Turkey, Chicken (86%), Xenopus, Drosophila (84%).

    Type of Immunogen: Synthetic peptide
    Isotype
    IgG
  • Application Notes
    Approved: IHC, IHC-P (10 μg/mL), WB

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
    Comment

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Distilled Water.
    Concentration
    Lot specific
    Buffer
    Lyophilized from PBS with 2 % sucrose
    Handling Advice
    Avoid repeat freeze-thaw cycles.
    Storage
    4 °C,-20 °C
    Storage Comment
    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target
    PLEKHH2 (Pleckstrin Homology Domain Containing, Family H (With MyTH4 Domain) Member 2 (PLEKHH2))
    Alternative Name
    PLEKHH2 (PLEKHH2 Products)
    Synonyms
    RGD1304935 antibody, si:ch73-105b23.3 antibody, PLEKHH1L antibody, AI256725 antibody, E030001K05 antibody, mKIAA2028 antibody, pleckstrin homology, MyTH4 and FERM domain containing H2 antibody, pleckstrin homology domain containing, family H (with MyTH4 domain) member 2 antibody, Plekhh2 antibody, plekhh2 antibody, PLEKHH2 antibody
    Background
    Name/Gene ID: PLEKHH2

    Synonyms: PLEKHH2, KIAA2028, PLEKHH1L
    Gene ID
    130271
    NCBI Accession
    NP_742066
    UniProt
    Q8IVE3
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