PLEKHH2 antibody (AA 1043-1092)
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- Target See all PLEKHH2 products
- PLEKHH2 (Pleckstrin Homology Domain Containing, Family H (With MyTH4 Domain) Member 2 (PLEKHH2))
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Binding Specificity
- AA 1043-1092
- Reactivity
- Human, Mouse, Rabbit, Cow, Dog, Guinea Pig, Horse, Bat, Monkey, Pig
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PLEKHH2 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human PLEKHH2
- Predicted Reactivity
- Percent identity by BLAST analysis: Dog, Bovine, Rabbit, Horse, Guinea pig (100%) Rat (92%) Chicken (86%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide located between aa1043-1092 of human PLEKHH2 (Q8IVE3, NP_742066). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Zebra finch (100%), Rat, Opossum (92%), Turkey, Chicken (86%), Xenopus, Drosophila (84%).
Type of Immunogen: Synthetic peptide - Isotype
- IgG
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- Application Notes
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Approved: IHC, IHC-P (10 μg/mL), WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Distilled Water.
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS with 2 % sucrose
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- Target
- PLEKHH2 (Pleckstrin Homology Domain Containing, Family H (With MyTH4 Domain) Member 2 (PLEKHH2))
- Alternative Name
- PLEKHH2 (PLEKHH2 Products)
- Synonyms
- RGD1304935 antibody, si:ch73-105b23.3 antibody, PLEKHH1L antibody, AI256725 antibody, E030001K05 antibody, mKIAA2028 antibody, pleckstrin homology, MyTH4 and FERM domain containing H2 antibody, pleckstrin homology domain containing, family H (with MyTH4 domain) member 2 antibody, Plekhh2 antibody, plekhh2 antibody, PLEKHH2 antibody
- Background
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Name/Gene ID: PLEKHH2
Synonyms: PLEKHH2, KIAA2028, PLEKHH1L - Gene ID
- 130271
- NCBI Accession
- NP_742066
- UniProt
- Q8IVE3
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