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Dynactin 1 antibody (C-Term)

Name: Dynactin 1 antibody (C-Term)
Brand: antibodies-online
SKU: ABIN184834
Price: 469.28 USD
Availability: InStock

Verified DCTN1 Reactivity: Human WB, ELISA, IHC, IF, FACS Host: Goat Polyclonal unconjugated

ABIN184834 staining (1µg/ml) of Human Testis lysate (RIPA buffer, 35µg total protein per lane).

ABIN184834 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.

IHC

ABIN184834 (1µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer).

ABIN184834 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.

FACS

ABIN184834 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.

7 images

(1 reference)

Catalog No. ABIN184834

Datasheet as PDF

Target See all Dynactin 1 (DCTN1) Antibodies

Dynactin 1 (DCTN1)
Binding Specificity
15

5

3

3

2

2

2

2

1

1

1

1

1

1
C-Term
Reactivity
52

20

14

5

4

3

2

2

2

1

1

1

1
Human
Host
39

11

3
Goat
Compatible Secondaries
Clonality
42

11
Polyclonal
Conjugate
32

2

2

2

2

2

2

2

1

1

1

1

1

1

1
This Dynactin 1 antibody is un-conjugated
Application
44

16

13

13

12

6

6

5

5

3

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose

Dynactin

Sequence

QEQLHQLHSR LIS

Specificity

This antibody is expected to recognise all reported isoforms.

Cross-Reactivity

Human, Mouse

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade

Verified

Immunogen

Peptide with sequence C-QEQLHQLHSRLIS, from the C Terminus of the protein sequence according to NP_004073.2, NP_075408.1, NP_001128512.1, NP_001128513.1.

Isotype

IgG
anti-Dynactin 1 (DCTN1) (C-Term) antibody
DCTN1 Reactivity: Human WB, IF, ICC, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Dynactin 1 (DCTN1) (AA 1084-1278) antibody
DCTN1 Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-Dynactin 1 (DCTN1) (C-Term) antibody
DCTN1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Dynactin 1 (DCTN1) (AA 945-1139) antibody
DCTN1 Reactivity: Human WB, IP Host: Rabbit Polyclonal unconjugated

anti-Dynactin 1 (DCTN1) (N-Term) antibody
DCTN1 Reactivity: Human WB, IP Host: Mouse Monoclonal unconjugated

anti-Dynactin 1 (DCTN1) (AA 1-198) antibody
DCTN1 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 2E4-1C2 unconjugated

anti-Dynactin 1 (DCTN1) antibody
DCTN1 Reactivity: Human, Mouse, Rat IF Host: Rabbit Polyclonal unconjugated

anti-Dynactin 1 (DCTN1) (AA 1216-1278) antibody
DCTN1 Reactivity: Human WB, IHC, ICC, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Dynactin 1 (DCTN1) (C-Term) antibody
DCTN1 Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig, Rabbit, Zebrafish (Danio rerio) WB Host: Rabbit Polyclonal unconjugated

anti-Dynactin 1 (DCTN1) (AA 251-350) antibody
DCTN1 Reactivity: Human WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Application Notes

Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 6-7 μg/mL.
Western Blot: Approx. 150 kDa band observed in HeLa and MCF7 cell lysates, and in preliminary testing of HEK293 and Mouse Brain lysate (calculated MW of 142 kDa according to NP_004073.2). Recommended concentration 0.3-1 μg/mL. Primary incubation 1 hour at
Peptide ELISA: antibody detection limit dilution 1:16000.

Comment

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells and additionally the nucleus of U2OS cells.. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeL

Restrictions

For Research Use only
Format

Liquid

Concentration

0.5 mg/mL

Buffer

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Minimize freezing and thawing.

Storage

-20 °C

Storage Comment

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Bupha-Intr, Haizlip, Janssen: "Temporal changes in expression of connexin 43 after load-induced hypertrophy in vitro." in: American journal of physiology. Heart and circulatory physiology, Vol. 296, Issue 3, pp. H806-14, (2009) (PubMed).
Target

Dynactin 1 (DCTN1)

Alternative Name

DCTN1 (DCTN1 Products)

Background

DCTN1, dynactin 1 (p150, glued homolog, Drosophila), P135, DP-150, DAP-150, p150-glued, p150, Glued (Drosophila) homolog, 150 kDa dynein-associated polypeptide, dynactin 1 (p150, Glued (Drosophila) homolog), HMN7B, OTTHUMP00000202491, dynactin 1, p150, Gl

Gene ID

1639, 13191

NCBI Accession

NP_004073, NP_075408, NP_001128512, NP_001128513, NP_001177765, NP_001177766

Pathways

M Phase, ER-Nucleus Signaling