Doublecortin antibody (AA 232-242)
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- Target See all Doublecortin (DCX) Antibodies
- Doublecortin (DCX)
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Binding Specificity
- AA 232-242
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Reactivity
- Human, Mouse, Rat
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This Doublecortin antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)
- Purpose
- Doublecortin / DCX (aa232-242)
- Sequence
- KTSANMKAPQ S
- Specificity
- This antibody is expected to recognize all four reported isoforms (NP_000546.2, NP_835365.1, NP_835364.1, NP_001182482.1). Reported variants represent identical protein: NP_835364.1 and NP_835366.1.
- Cross-Reactivity
- Cow, Dog, Human, Mouse, Pig, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- CKTSANMKAPQS
- Isotype
- IgG
- Top Product
- Discover our top product DCX Primary Antibody
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anti-Doublecortin (DCX) (AA 362-411) antibody
DCX Reactivity: Human WB, ELISA, IHC, ICC, FACS Host: Mouse Monoclonal 2G5 unconjugated
anti-Doublecortin (DCX) (AA 362-411) antibodyDCX Reactivity: Human WB, ELISA, IHC, FACS, IF/ICC Host: Mouse Monoclonal unconjugated
anti-Doublecortin (DCX) (AA 69-81) antibodyVerified DCX Reactivity: Human ELISA, IHC, IF, FACS Host: Goat Polyclonal unconjugated
anti-Doublecortin (DCX) (AA 1-360) antibodyDCX Reactivity: Human WB, ELISA, IF Host: Mouse Monoclonal 1G12 unconjugated
anti-Doublecortin (DCX) (AA 108-360) antibodyDCX Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Doublecortin (DCX) antibodyDCX Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-Doublecortin (DCX) (AA 11-360) antibodyDCX Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated
anti-Doublecortin (DCX) (pSer335) antibodyDCX Reactivity: Human, Mouse, Rat WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-Doublecortin (DCX) (Ser30) antibodyDCX Reactivity: Human, Mouse, Rat WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-Doublecortin (DCX) (AA 107-137) antibodyDCX Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB15193 unconjugated
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- Application Notes
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Immunohistochemistry: In paraffin embedded Human Cerebellum shows axonal and incidental nuclear staining. Recommended concentration: 2-4 μg/mL. This antibody has been successfully used in IHC on Rat: Heysieattalab, S et al. (2021) Acta Epileptologica 3, 3
Western Blot: Approx 45 kDa band observed in Mouse fetal Brain lysates (calculated MW of 49.3 kDa according to Mouse NP_001103692.1). Recommended concentration: 0.01-0.03 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:32000.
- Comment
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 and KNRK cells. Recommended concentration: 5-10μg/ml.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- Doublecortin (DCX)
- Alternative Name
- DCX (DCX Products)
- Background
- DCX, doublecortin, RP5-914P14.1, DBCN, DC, FLJ51296, LISX, SCLH, XLIS, OTTHUMP00000023860, OTTHUMP00000216316, doublecortex, doublin, lis-X, lissencephalin-X, neuronal migration protein doublecortin
- Gene ID
- 1641, 13193, 84394
- NCBI Accession
- NP_000546, NP_835365, NP_835364, NP_001182482
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