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E2F3 antibody (C-Term)

Name: E2F3 antibody (C-Term)
SKU: ABIN2780388
Price: 472.77 USD
Availability: InStock

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit WB Host: Rabbit Polyclonal unconjugated

WB Suggested Anti-E2F3 Antibody Titration: 0.

Host: Mouse Target Name: E2F3 Sample Tissue: Mouse Small Intestine Antibody Dilution: 1ug/ml

Host: Rabbit Target Name: E2F3 Sample Tissue: Human Lung Tumor Antibody Dilution: 1ug/ml

Host: Rabbit Target Name: E2F3 Sample Tissue: Human Ovary Tumor Antibody Dilution: 1.

4 images

(3 references)

Catalog No. ABIN2780388

Datasheet as PDF

Target See all E2F3 Antibodies

E2F3
Binding Specificity
15

15

15

8

7

6

6

5

4

2

2

2

2

2

1

1

1

1

1
C-Term
Reactivity
65

58

37

2

2

2

2

2

2

1

1

1

1
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
Host
80

4

1
Rabbit
Compatible Secondaries
Clonality
82

3
Polyclonal
Conjugate
27

8

7

6

3

3

3

3

3

3

3

3

3

3

3

2

2
This E2F3 antibody is un-conjugated
Application
79

39

39

38

11

9

4

2

2

1

1
Western Blotting (WB)

Sequence

LLQQTEDQIP SNLEGPFVNL LPPLLQEDYL LSLGEEEGIS DLFDAYDLEK

Specificity

The immunizing peptide used to raise this antibody is 100 % homologous to isoform 2 (334aa, 37 kDa) of human E2F3.

Predicted Reactivity

Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 92%, Rat: 100%

Characteristics

This is a rabbit polyclonal antibody against E2F3. It was validated on Western Blot using a cell lysate as a positive control.

Purification

Affinity Purified

Immunogen

The immunogen is a synthetic peptide directed towards the C terminal region of human E2F3

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anti-E2F3 (AA 336-425) antibody
Reactivity: Human WB, ELISA, PLA Host: Mouse Monoclonal 5F7 unconjugated

anti-E2F3 (AA 381-465) antibody
Reactivity: Human, Mouse WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-E2F3 (C-Term) antibody
Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Bat, Pig, Hamster WB, IHC (p), IHC Host: Rabbit Polyclonal unconjugated

anti-E2F3 (acLys168) antibody
Reactivity: Human WB, ELISA Host: Rabbit Polyclonal unconjugated

anti-E2F3 (AA 1-108) antibody
Reactivity: Human ELISA, IF Host: Rabbit Polyclonal unconjugated

anti-E2F3 (AA 1-133) antibody
Reactivity: Human WB, ELISA Host: Mouse Polyclonal unconjugated

anti-E2F3 antibody
Reactivity: Mouse, Rat WB Host: Rabbit Polyclonal unconjugated

anti-E2F3 (C-Term) antibody
Verified Reactivity: Mouse WB, ELISA Host: Goat Polyclonal unconjugated

anti-E2F3 (AA 1-133) antibody
Reactivity: Human ELISA Host: Mouse Monoclonal 3C11 unconjugated

anti-E2F3 antibody
KO Validated Reactivity: Human WB Host: Rabbit Polyclonal unconjugated

Application Notes

Optimal working dilutions should be determined experimentally by the investigator.

Comment

Antigen size: 334 AA

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freeze-thaw cycles.

Storage

-20 °C

Storage Comment

For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Giangreco, Vaishnav, Wagner, Finelli, Fleshner, Van der Kwast, Vieth, Nonn: "Tumor suppressor microRNAs, miR-100 and -125b, are regulated by 1,25-dihydroxyvitamin D in primary prostate cells and in patient tissue." in: Cancer prevention research (Philadelphia, Pa.), Vol. 6, Issue 5, pp. 483-94, (2013) (PubMed).

Melcher, Hartmann, Zopf, Herterich, Wilke, Müller, Rosler, Kudlich, Al-Taie, Rosenwald, Katzenberger, Scholtka, Seibold, Rogoll, Scheppach, Scheurlen, Lührs: "LOH and copy neutral LOH (cnLOH) act as alternative mechanism in sporadic colorectal cancers with chromosomal and microsatellite instability." in: Carcinogenesis, Vol. 32, Issue 4, pp. 636-42, (2011) (PubMed).

Naga Prasad, Duan, Gupta, Surampudi, Volinia, Calin, Liu, Kotwal, Moravec, Starling, Perez, Sen, Wu, Plow, Croce, Karnik: "Unique microRNA profile in end-stage heart failure indicates alterations in specific cardiovascular signaling networks." in: The Journal of biological chemistry, Vol. 284, Issue 40, pp. 27487-99, (2009) (PubMed).
Target

E2F3

Abstract

E2F3 Products

Synonyms

E2F-3 antibody, E2F3b antibody, E2f3a antibody, mKIAA0075 antibody, RGD1561600 antibody, E2F transcription factor 3 antibody, E2F transcription factor 3 S homeolog antibody, E2F3 antibody, e2f3.S antibody, E2f3 antibody

Background

E2F3 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F2, have an additional cyclin binding domain. E2F3 binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner.
Alias Symbols: E2F-3
Protein Interaction Partner: RB1, TRIM28, TFDP1, HDAC1, SRA1, RBL1, FZR1, ELAVL1, PCNA, MCM4, HELLS, CCNB1, BCL6, ATAD2, HCFC1, USF1, KMT2D, RYBP, TFDP2, YY1, TFE3, SP1, CCNA2, CDK3, RNF144A, FHL2, WNK1, MGA, TEAD3, SPIB, PKIB, MYBL2, CREBBP, EAPP, JMY, PPP1R13B, TP53BP2, TP53INP1, B
Protein Size: 465

Molecular Weight

49 kDa

Gene ID

1871

NCBI Accession

NM_001243076

UniProt

Q68DT0