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EPH Receptor A2 antibody (N-Term)

Name: EPH Receptor A2 antibody (N-Term)
SKU: ABIN391885
Price: 429 USD
Availability: InStock

EPHA2 Reactivity: Human, Mouse WB, FACS, IHC (p) Host: Rabbit Polyclonal RB01579 unconjugated

Anti-EPHA2 Antibody (T45) at 1:1000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 μg per lane.

Flow cytometric analysis of NCI- cells using EphA2 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram).

FACS

Western blot analysis of hEPHA2-T45 (ABIN391885 and ABIN2841705) in MCF-7 cell line lysates (35 μg/lane).

Western blot analysis of hEPHA2-T45 (ABIN391885 and ABIN2841705) in mouse NIH-3T3 cell line lysates (35 μg/lane).

Western blot analysis of EPHA2 (arrow) using rabbit polyclonal hEPHA2-T45 (ABIN391885 and ABIN2841705).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

IHC (p)

6 images

(1 reference)

Catalog No. ABIN391885

Datasheet as PDF

Target See all EPH Receptor A2 (EPHA2) Antibodies

EPH Receptor A2 (EPHA2)
Binding Specificity
19

15

15

15

10

10

9

9

8

8

8

7

7

7

7

7

7

6

6

6

5

4

4

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
AA 30-60, N-Term
Reactivity
180

128

70

14

6

5

4

3

2

1

1

1

1

1
Human, Mouse
Host
190

17

4

1

1
Rabbit
Compatible Secondaries
Clonality
187

25

1
Polyclonal
Conjugate
72

20

12

12

12

12

12

12

11

10

9

9

2

2

2

2

2
This EPH Receptor A2 antibody is un-conjugated
Application
130

80

27

26

21

14

12

8

7

7

7

3

3

3

2

2

1

1
Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity

Pr

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen

This EphA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-60 amino acids from the N-terminal region of human EphA2.

Clone

RB01579

Isotype

Ig Fraction
anti-EPH Receptor A2 (EPHA2) (AA 901-976) antibody
EPHA2 Reactivity: Human, Mouse, Rat WB, ELISA, IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-EPH Receptor A2 (EPHA2) (AA 204-326) antibody
EPHA2 Reactivity: Human WB, ELISA, IF Host: Mouse Monoclonal 1E3 unconjugated

anti-EPH Receptor A2 (EPHA2) antibody
EPHA2 Reactivity: Human FACS, IF, cELISA Host: Mouse Monoclonal Kalpha-5H5 unconjugated

anti-EPH Receptor A2 (EPHA2) (AA 30-60) antibody
EPHA2 Reactivity: Human, Mouse WB, ELISA, IHC, FACS Host: Rabbit Polyclonal unconjugated

anti-EPH Receptor A2 (EPHA2) (AA 333-347), (Extracellular), (N-Term) antibody
EPHA2 Reactivity: Human, Mouse, Rat WB, IHC, FACS Host: Rabbit Polyclonal unconjugated

anti-EPH Receptor A2 (EPHA2) antibody
EPHA2 Reactivity: Human WB, ELISA, IHC Host: Mouse Monoclonal 1B3C7 unconjugated

anti-EPH Receptor A2 (EPHA2) (AA 559-976) antibody
EPHA2 Reactivity: Human, Mouse WB, ELISA, IHC, IHC (p) Host: Mouse Monoclonal 3D7 unconjugated

anti-EPH Receptor A2 (EPHA2) (AA 847-976) antibody
EPHA2 Reactivity: Human WB Host: Rabbit Polyclonal unconjugated

anti-EPH Receptor A2 (EPHA2) (AA 627-976) antibody
EPHA2 Reactivity: Human ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-EPH Receptor A2 (EPHA2) (pTyr594) antibody
EPHA2 Reactivity: Human WB, ELISA, IF (p), IHC (p), IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated

Application Notes

WB: 1:1000. WB: 1:1000. WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100. FC: 1:10~50

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date

6 months
Tatsukawa, Koga, Aoki, Koshikawa, Imafuku, Nakayama, Nabeshima: "Immunohistochemical demonstration of EphA2 processing by MT1-MMP in invasive cutaneous squamous cell carcinoma." in: Virchows Archiv : an international journal of pathology, Vol. 469, Issue 1, pp. 25-34, (2017) (PubMed).
Target

EPH Receptor A2 (EPHA2)

Alternative Name

EphA2 (EPHA2 Products)

Background

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).

Molecular Weight

108266

Gene ID

1969

NCBI Accession

NP_004422

UniProt

P29317

Pathways

RTK Signaling