SIRPA Protein (AA 31-370) (His tag)
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- Target See all SIRPA Proteins
- SIRPA (Signal-Regulatory Protein alpha (SIRPA))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 31-370
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This SIRPA protein is labelled with His tag.
- Purpose
- Human SIRP alpha/CD172a Protein
- Sequence
- Glu31-Arg370
- Characteristics
- Recombinant Human SIRP alpha/CD172a Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu31-Arg370.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human SIRP alpha, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 38.1ng/ml determined by ELISA. The affinity constant of 20.6nM as determined in SPR assay (Biacore T200). See testing image for detail.
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Signal-Regulatory Protein alpha (SIRPA) (AA 31-370) protein (Fc Tag)
SIRPA Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by SEC-HPLC
Signal-Regulatory Protein alpha (SIRPA) (AA 31-370) protein (His tag)SIRPA Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Signal-Regulatory Protein alpha (SIRPA) protein (Fc-His Tag)SIRPA Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
Signal-Regulatory Protein alpha (SIRPA) protein (Fc-Avi Tag,Biotin)SIRPA Origin: Human Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Signal-Regulatory Protein alpha (SIRPA) (AA 32-373) protein (His tag)SIRPA Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Signal-Regulatory Protein alpha (SIRPA) (AA 31-370) (Active) protein (His tag)SIRPA Origin: Cynomolgus Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Signal-Regulatory Protein alpha (SIRPA) (Active) protein (His tag)SIRPA Origin: Human Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE. Active
Signal-Regulatory Protein alpha (SIRPA) (Active) protein (His tag)SIRPA Origin: Cynomolgus Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE. Active
Signal-Regulatory Protein alpha (SIRPA) (Active) protein (Biotin,His-Avi Tag)SIRPA Origin: Human Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE. Active
Signal-Regulatory Protein alpha (SIRPA) (AA 31-370) (Active) protein (Fc Tag)SIRPA Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- SIRPA (Signal-Regulatory Protein alpha (SIRPA))
- Alternative Name
- SIRP alpha (SIRPA Products)
- Synonyms
- BIT Protein, CD172A Protein, MFR Protein, MYD-1 Protein, P84 Protein, PTPNS1 Protein, SHPS1 Protein, SIRP Protein, SIRPA Protein, SIRP-A1 Protein, SIRP-B1 Protein, AI835480 Protein, Bit Protein, CD172a Protein, Ptpns1 Protein, SHP-1 Protein, SHPS-1 Protein, swc3 Protein, signal regulatory protein alpha Protein, myeloperoxidase Protein, signal-regulatory protein alpha Protein, tyrosine-protein phosphatase non-receptor type substrate 1 Protein, SIRPA Protein, MPO Protein, LOC458039 Protein, LOC100400378 Protein, LOC100590224 Protein, Sirpa Protein
- Background
- Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.
- Molecular Weight
- 38.1 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.
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