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PARP1 antibody

Name: PARP1 antibody
Brand: antibodies-online
SKU: ABIN2854798
Price: 609.18 USD
Availability: InStock

KO Validated PARP1 Reactivity: Human WB, IP, IF, IHC (p), ICC, ChIP Host: Rabbit Polyclonal unconjugated

WB Image PARP1 antibody detects PARP1 protein by Western blot analysis.

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PARP1, antibody at 1:200 dilution.

ChIP

WB Image Sample (30 ug of whole cell lysate) A: 293T B: HeLa 7.

Expression of apoptogenic proteins in MCME-treated cancer cells.

Combined treatment of gefitinib and lovastatin induced apoptosis and autophagy in SSP-25 cellsThe cells (1 x 106/well) were seeded in a six-well tray and treated with a combination of gefitinib and lovastatin for 24 hours.

GSH inhibited the activation of Cd-induced p38 MAPK/JNK pathways and proteins involved in apoptosis signalingBEAS-2B cells were sham-exposed or dosed with 30 μM CdCl2 for 6, 12, or 24 h (A) or with 30 μM CdCl2 for 24 or 36 h (B), cells were lysed, and protein extracts were subjected to western blot analyses using antibodies against p-p38 MAPK, p38 MAPK, p-JNK, JNK, p-c-Jun, c-Jun, Bax, Cytochrome c, Caspase-9, Caspase-3, and PARP1.

The association of PARP-1 with CCR6DNP is sequence dependent and allele specific.

ChIP

CCR6 expression in PARP-1 knockdown human cells.

CCR6 expression in human cells treated with 3-aminobenzamide.

Partial characterization of DSB repair complexes by co-IP analysis.

CISD2 expression affects cell apoptosis.

17 images

(7 references)

Catalog No. ABIN2854798

Datasheet as PDF

Target See all PARP1 Antibodies

PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))
Reactivity
232

64

49

8

6

6

5

5

5

3

3

2

2

1

1
Human
Host
221

27

1

1
Rabbit
Compatible Secondaries
Clonality
207

43
Polyclonal
Conjugate
144

17

16

16

15

14

3

3

3

3

3

3

2

2

2

2

2
This PARP1 antibody is un-conjugated
Application
161

130

69

39

35

27

27

23

23

17

16

7

7

2

2

2

1
Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP)

Cross-Reactivity

Human, Mouse, Rat

Characteristics

Rabbit Polyclonal antibody to PARP (poly (ADP-ribose) polymerase 1)
PARP antibody

Purification

Purified by antigen-affinity chromatography.

Grade

KO Validated

Immunogen

Recombinant protein encompassing a sequence within the center region of human PARP1. The exact sequence is proprietary.

Isotype

IgG
anti-Poly (ADP-Ribose) Polymerase 1 (PARP1) (AA 81-390) antibody
PARP1 Reactivity: Human WB, IHC, IP, IF Host: Rabbit Polyclonal unconjugated

anti-Poly (ADP-Ribose) Polymerase 1 (PARP1) (AA 140-220), (Cleaved-Asp214) antibody
PARP1 Reactivity: Human, Mouse WB, ELISA, IF, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Poly (ADP-Ribose) Polymerase 1 (PARP1) (AA 661-881) antibody
PARP1 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Poly (ADP-Ribose) Polymerase 1 (PARP1) (AA 201-300) antibody
PARP1 Reactivity: Human, Mouse, Rat, Chicken WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Poly (ADP-Ribose) Polymerase 1 (PARP1) (AA 1-100) antibody
PARP1 Reactivity: Human WB, ELISA, IF Host: Mouse Monoclonal 3G4 unconjugated

anti-Poly (ADP-Ribose) Polymerase 1 (PARP1) (C-Term) antibody
PARP1 Reactivity: Human WB Host: Rabbit Polyclonal unconjugated

anti-Poly (ADP-Ribose) Polymerase 1 (PARP1) (AA 670-858) antibody
PARP1 Reactivity: Human, Mouse, Rat WB, IHC (p), ICC Host: Rabbit Polyclonal unconjugated

anti-Poly (ADP-Ribose) Polymerase 1 (PARP1) (cleaved) antibody
PARP1 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Poly (ADP-Ribose) Polymerase 1 (PARP1) antibody
PARP1 Reactivity: Human WB, ELISA, IHC, FACS, IF Host: Rabbit Monoclonal 8C7 unconjugated Recombinant Antibody

anti-Poly (ADP-Ribose) Polymerase 1 (PARP1) antibody
PARP1 Reactivity: Human WB, IHC Host: Rabbit Monoclonal unconjugated

Application Notes

WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment

Positive Control: HCT116 (30uM cisplatin treatment for 24hr) , PC-12 , Rat2 , 293T , A431 , HeLa , HepG2
Validation: Comparison, KO/KD, Orthogonal, Overexpression

Restrictions

For Research Use only
Format

Liquid

Concentration

0.3 mg/mL

Buffer

1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Takino, Sato, Nagamine, Hori: "The inhibition of Bax activation-induced apoptosis by RasGRP2 via R-Ras-PI3K-Akt signaling pathway in the endothelial cells." in: Scientific reports, Vol. 9, Issue 1, pp. 16717, (2019) (PubMed).

Bera, Chiu, Huang, Liang, Lee, Shih: "Genetic and Epigenetic Perturbations by DNMT3A-R882 Mutants Impaired Apoptosis through Augmentation of PRDX2 in Myeloid Leukemia Cells." in: Neoplasia (New York, N.Y.), Vol. 20, Issue 11, pp. 1106-1120, (2019) (PubMed).

Dominguez, Maddelein, Pucelle, Nicaise, Maurage, Duyckaerts, Cuvillier, Delisle: "Neuronal sphingosine kinase 2 subcellular localization is altered in Alzheimer's disease brain." in: Acta neuropathologica communications, Vol. 6, Issue 1, pp. 25, (2019) (PubMed).

Li, Chen, Chen, Yen, Fang, Tsai, Chang, Shen, Huang, Chuu, Chang, Hsiung, Jiang: "Upregulation of CISD2 augments ROS homeostasis and contributes to tumorigenesis and poor prognosis of lung adenocarcinoma." in: Scientific reports, Vol. 7, Issue 1, pp. 11893, (2019) (PubMed).

Chakraborty, Tapryal, Venkova, Horikoshi, Pandita, Sarker, Sarkar, Pandita, Hazra: "Classical non-homologous end-joining pathway utilizes nascent RNA for error-free double-strand break repair of transcribed genes." in: Nature communications, Vol. 7, pp. 13049, (2018) (PubMed).

Liang, Shi, Yang, Qiu, Lin, Qi, Li, Zhao, Liu: "Inhibition of polypyrimidine tract-binding protein 3 induces apoptosis and cell cycle arrest, and enhances the cytotoxicity of 5- fluorouracil in gastric cancer cells." in: British journal of cancer, Vol. 116, Issue 7, pp. 903-911, (2017) (PubMed).

Xu, Wu, Zheng, Yu, Yang, Yao, Zhou, Ching, Lau: "Proteome profiling of cadmium-induced apoptosis by antibody array analyses in human bronchial epithelial cells." in: Oncotarget, Vol. 7, Issue 5, pp. 6146-58, (2017) (PubMed).
Target

PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Alternative Name

poly(ADP-ribose) polymerase 1 (PARP1 Products)

Synonyms

ADPRT antibody, ADPRT 1 antibody, ADPRT1 antibody, ARTD1 antibody, PARP antibody, PARP-1 antibody, PPOL antibody, pADPRT-1 antibody, Adprt antibody, Parp-1 antibody, 5830444G22Rik antibody, AI893648 antibody, Adprp antibody, Adprt1 antibody, C80510 antibody, parp-1 antibody, sPARP-1 antibody, BEST:LD21673 antibody, CG17685 antibody, CG17696 antibody, CG17718 antibody, CG40411 antibody, D.PARP antibody, Dm.pARTa antibody, Dmel\\CG40411 antibody, LD21673.3prime antibody, PARP1 antibody, dPARP antibody, parp antibody, adprt1 antibody, padprt-1 antibody, ppol antibody, si:dkey-206f10.3 antibody, wu:fc60f12 antibody, zgc:110092 antibody, poly(ADP-ribose) polymerase 1 antibody, poly (ADP-ribose) polymerase 1 antibody, poly (ADP-ribose) polymerase family, member 1 antibody, Poly-(ADP-ribose) polymerase antibody, poly(ADP-ribose) polymerase 1 L homeolog antibody, PARP1 antibody, Parp1 antibody, Parp antibody, parp1.L antibody, parp1 antibody

Background

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.

Cellular Localization: Nucleus

Molecular Weight

113 kDa

Gene ID

142

UniProt

P09874

Pathways

Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location