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CK1 epsilon antibody (C-Term)

Verified CSNK1E Reactivity: Human WB, ELISA, IHC, IF, FACS Host: Goat Polyclonal unconjugated
Catalog No. ABIN238540
  • Target See all CK1 epsilon (CSNK1E) Antibodies
    CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))
    Binding Specificity
    • 10
    • 9
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 58
    • 41
    • 17
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 60
    • 7
    • 2
    Goat
    Clonality
    • 62
    • 7
    Polyclonal
    Conjugate
    • 36
    • 6
    • 6
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This CK1 epsilon antibody is un-conjugated
    Application
    • 46
    • 32
    • 31
    • 12
    • 11
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
    Purpose
    CSNK1E
    Sequence
    PASQTSVPFD HLGK
    Cross-Reactivity
    Dog, Human, Mouse, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-PASQTSVPFDHLGK, from the C Terminus of the protein sequence according to NP_001885.1, NP_689407.1.
    Isotype
    IgG
  • Application Notes

    Immunohistochemistry: Paraffin embedded Human Tonsil. Recommended concentration: 5 μg/mL.

    Western Blot: Approx 48 kDa band observed in nuclear lysates of cell lines A431and HeLa, and approx 45 kDa in nuclear lysates of cell line K562 (calculated MW of 47.3 kDa according to NP_001276841.1). Recommended concentration: 0.3-1 μg/mL. Primary incubatio

    Peptide ELISA: antibody detection limit dilution 1:32000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and A431 cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))
    Alternative Name
    CSNK1E (CSNK1E Products)
    Synonyms
    CKIepsilon antibody, HCKIE antibody, CKIe antibody, ck1-epsilon antibody, ck1epsilon antibody, hckie antibody, dco antibody, fj38c01 antibody, wu:fj38c01 antibody, wu:fj62a04 antibody, zgc:77310 antibody, AI426939 antibody, AI551861 antibody, AW457082 antibody, CK1epsilon antibody, KC1epsilon antibody, tau antibody, ckie antibody, CSNK1E antibody, Csnk1e antibody, CKI-epsilon antibody, 0538/13 antibody, 0915/10 antibody, 1396/02 antibody, 1447/01 antibody, 1460/09 antibody, CG2048 antibody, CK1delta/epsilon antibody, DBT antibody, DBT/CK1epsilon antibody, DCO antibody, DCO/CKIe antibody, Dbt antibody, Dco antibody, Dco/CK1 antibody, Dmel\CG2048 antibody, dCKIepsilon antibody, dbt antibody, dco-1 antibody, ddbt antibody, l(3)S053813 antibody, l(3)S144701 antibody, l(3)dco antibody, l(3)dco-1 antibody, l(3)discs overgrown antibody, l(3)discs overgrown-1 antibody, l(3)j3B9 antibody, l(3)rK215 antibody, casein kinase 1 epsilon antibody, casein kinase 1, epsilon antibody, casein kinase 1 epsilon L homeolog antibody, casein kinase I isoform epsilon antibody, casein kinase I epsilon antibody, casein kinase I antibody, LOC400927-CSNK1E readthrough antibody, discs overgrown antibody, CSNK1E antibody, csnk1e antibody, Csnk1e antibody, csnk1e.L antibody, LOC443240 antibody, NAEGRDRAFT_82890 antibody, LOC100407384 antibody, LOC100523244 antibody, LOC100607246 antibody, LOC100727500 antibody, LOC400927-CSNK1E antibody, dco antibody
    Background
    CSNK1E, casein kinase 1, epsilon, RP1-5O6.1, HCKIE, MGC10398 , CKI-epsilon, OTTHUMP00000063258, OTTHUMP00000063263, casein kinase 1 epsilon, casein kinase I epsilon
    Gene ID
    1454, 27373, 58822
    NCBI Accession
    NP_001276841
    Pathways
    Hedgehog Signaling, M Phase
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