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Versican antibody (AA 3301-3396)

Name: Versican antibody (AA 3301-3396)
SKU: ABIN741660
Price: 384.62 USD
Availability: InStock

Vcan Reactivity: Mouse, Rat ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin-embedded rat brain labeled with Rabbit Anti-Versican Polyclonal Antibody, Unconjugated (ABIN741660) 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC

Formalin-fixed and paraffin-embedded mouse brain labeled with Rabbit Anti-Versican Polyclonal Antibody, Unconjugated (ABIN741660) 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC

Paraformaldehyde-fixed, paraffin embedded rat brain, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Versican Polyclonal Antibody (bs-2533R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining

IHC (p)

Paraformaldehyde-fixed, paraffin embedded mouse brain, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Versican Polyclonal Antibody (bs-2533R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.

IHC (p)

Formalin-fixed and paraffin-embedded mouse brain labeled with Rabbit Anti-Versican Polyclonal Antibody, Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC (p)

Formalin-fixed and paraffin-embedded rat brain labeled with Rabbit Anti-Versican Polyclonal Antibody, Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC (p)

6 images

Catalog No. ABIN741660

Datasheet as PDF

Target See all Versican (Vcan) Antibodies

Versican (Vcan)
Binding Specificity
15

14

3

2

2

2

1

1

1

1

1

1

1

1

1
AA 3301-3396
Reactivity
44

36

19

4

1

1
Mouse, Rat
Host
42

24

1

1
Rabbit
Compatible Secondaries
Clonality
38

30
Polyclonal
Conjugate
28

4

4

3

3

3

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
This Versican antibody is un-conjugated
Application
35

18

16

15

13

12

12

8

5

2

1

1

1
ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity

Mouse, Rat

Predicted Reactivity

Human,Dog,Cow,Pig,Horse

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human Versican core protein

Isotype

IgG

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anti-Versican (Vcan) (AA 3058-3299) antibody
Vcan Reactivity: Mouse WB, ICC, IHC, IP Host: Rabbit Polyclonal unconjugated

anti-Versican (Vcan) (AA 2958-3209) antibody
Vcan Reactivity: Human WB, ICC, IHC, IP Host: Rabbit Polyclonal unconjugated

anti-Versican (Vcan) (AA 362-585) antibody
Vcan Reactivity: Mouse WB, ICC, IF Host: Mouse Monoclonal S351-23 unconjugated

anti-Versican (Vcan) (AA 362-585) antibody (HRP)
Vcan Reactivity: Mouse WB, ICC, IF Host: Mouse Monoclonal S351-23 HRP

anti-Versican (Vcan) (AA 362-585) antibody (FITC)
Vcan Reactivity: Mouse WB, ICC, IF Host: Mouse Monoclonal S351-23 FITC

anti-Versican (Vcan) (AA 362-585) antibody (Alkaline Phosphatase (AP))
Vcan Reactivity: Mouse WB, ICC, IF Host: Mouse Monoclonal S351-23 Alkaline Phosphatase (AP)

anti-Versican (Vcan) (AA 362-585) antibody (Biotin)
Vcan Reactivity: Mouse WB, ICC, IF Host: Mouse Monoclonal S351-23 Biotin

anti-Versican (Vcan) (AA 362-585) antibody (PE)
Vcan Reactivity: Mouse WB, ICC, IF Host: Mouse Monoclonal S351-23 PE

anti-Versican (Vcan) (AA 362-585) antibody (APC)
Vcan Reactivity: Mouse WB, ICC, IF Host: Mouse Monoclonal S351-23 APC

anti-Versican (Vcan) (AA 362-585) antibody (PerCP)
Vcan Reactivity: Mouse WB, ICC, IF Host: Mouse Monoclonal S351-23 PerCP

Application Notes

ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Target

Versican (Vcan)

Alternative Name

Versican (Vcan Products)

Background

Synonyms: WGN, ERVR, GHAP, PG-M, WGN1, CSPG2, Versican core protein, Chondroitin sulfate proteoglycan core protein 2, Chondroitin sulfate proteoglycan 2, Glial hyaluronate-binding protein, Large fibroblast proteoglycan, VCAN
Background: May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid.

Gene ID

1462

UniProt

P13611

Pathways

Glycosaminoglycan Metabolic Process