GCLC antibody (N-Term)
(1 reference)-
- Target See all GCLC Antibodies
- GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))
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Binding Specificity
- N-Term
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Reactivity
- Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Rabbit, Dog, Guinea Pig, Horse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This GCLC antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC)
- Sequence
- VLETLQEKGE RTNPNHPTLW RPEYGSYMIE GTPGQPYGGT MSEFNTVEAN
- Predicted Reactivity
- Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%
- Characteristics
- This is a rabbit polyclonal antibody against GCLC. It was validated on Western Blot using a cell lysate as a positive control.
- Purification
- Affinity Purified
- Immunogen
- The immunogen is a synthetic peptide directed towards the N terminal region of human GCLC
- Top Product
- Discover our top product GCLC Primary Antibody
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anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 528-637) antibody
GCLC Reactivity: Human WB, ELISA, IF Host: Mouse Monoclonal 3H1 unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 1-30), (N-Term) antibodyGCLC Reactivity: Human WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB31687 unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 1-30) antibodyGCLC Reactivity: Human WB, IHC, ELISA, IF, FACS Host: Rabbit Polyclonal unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (N-Term) antibodyGCLC Reactivity: Human, Mouse, Rat, Pig WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 437-637) antibodyGCLC Reactivity: Human, Rat WB, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) antibodyGCLC Reactivity: Human WB, IHC Host: Rabbit Monoclonal unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 1-252) antibodyGCLC Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 435-636) antibodyGCLC Reactivity: Rat WB, IHC, ICC Host: Rabbit Polyclonal unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (Middle Region) antibodyGCLC Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Rabbit, Dog, Guinea Pig, Horse, Saccharomyces cerevisiae WB, IHC Host: Rabbit Polyclonal unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 6-34), (N-Term) antibodyGCLC Reactivity: Human WB, IF, FACS, IHC (p), EIA Host: Rabbit Polyclonal unconjugated
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- Application Notes
- Optimal working dilutions should be determined experimentally by the investigator.
- Comment
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Antigen size: 637 AA
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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: "Molecular mechanisms of lipopolysaccharide-mediated inhibition of glutathione synthesis in mice." in: Free radical biology & medicine, Vol. 68, pp. 148-58, (2014) (PubMed).
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: "Molecular mechanisms of lipopolysaccharide-mediated inhibition of glutathione synthesis in mice." in: Free radical biology & medicine, Vol. 68, pp. 148-58, (2014) (PubMed).
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- Target
- GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))
- Alternative Name
- GCLC (GCLC Products)
- Synonyms
- GCLC antibody, gclc antibody, MGC146287 antibody, 2259 antibody, CG2259 antibody, DmGCLC antibody, DmGCS antibody, DmGCSh antibody, Dmel\\CG2259 antibody, GCL antibody, GCLc antibody, GCSh antibody, Gcs antibody, Gcsh antibody, DDBDRAFT_0186120 antibody, DDBDRAFT_0231403 antibody, DDB_0186120 antibody, DDB_0231403 antibody, GCS antibody, GLCL antibody, GLCLC antibody, D9Wsu168e antibody, GLCL-H antibody, Ggcs-hs antibody, Glclc antibody, cb1049 antibody, fe36e11 antibody, wu:fe36e11 antibody, glutamate-cysteine ligase catalytic subunit antibody, glutamate-cysteine ligase, catalytic subunit antibody, Glutamate-cysteine ligase catalytic subunit antibody, gamma glutamylcysteine synthetase antibody, glutamate-cysteine ligase, catalytic subunit L homeolog antibody, glutamate-cysteine ligase, catalytic subunit S homeolog antibody, gamma-glutamylcysteine synthetase antibody, GCLC antibody, gclc antibody, Gclc antibody, LbGCS antibody, gcsA antibody, gclc.L antibody, gclc.S antibody, GSH1 antibody
- Background
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Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. GCLC is the catalytic subunit of 637 amino acids with a calculated molecular weight of 72.773 kDa. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6. The regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: GCS, GLCL, GLCLC, GCL
Protein Interaction Partner: UBC, PPID, GCLM, CCL22, PAXIP1, ELAVL1,
Protein Size: 637 - Molecular Weight
- 73 kDa
- Gene ID
- 2729
- NCBI Accession
- NM_001498, NP_001489
- UniProt
- P48506
- Pathways
- Cell RedoxHomeostasis
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