C5 antibody (N-Term)
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- Target See all C5 Antibodies
- C5 (Complement Component 5 (C5))
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Binding Specificity
- AA 1-18, N-Term
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Reactivity
- Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This C5 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purpose
- Rabbit IgG polyclonal antibody for Complement C5(C5) detection. Tested with WB, IHC-P in Mouse,Rat.
- Sequence
- DLQLLHQKVE EQAAKYKH
- Cross-Reactivity (Details)
- No cross reactivity with other proteins.
- Characteristics
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Rabbit IgG polyclonal antibody for Complement C5(C5) detection. Tested with WB, IHC-P in Mouse,Rat.
Gene Name: complement component 5
Protein Name: Complement C5 - Purification
- Immunogen affinity purified.
- Immunogen
- A synthetic peptide corresponding to a sequence at the N-terminus of rat C5a(1-18aa DLQLLHQKVEEQAAKYKH), different from the related mouse sequence by four amino acids.
- Isotype
- IgG
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anti-Complement Component 5 (C5) (AA 701-755) antibody
C5 Reactivity: Human, Mouse WB, ELISA, IF (p), IF (cc), IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Complement Component 5 (C5) (AA 253-281), (N-Term) antibodyC5 Reactivity: Human WB, IHC (p), FACS Host: Rabbit Polyclonal RB21866 unconjugated
anti-Complement Component 5 (C5) (AA 253-281) antibodyC5 Reactivity: Human WB, ELISA, IHC, FACS Host: Rabbit Polyclonal unconjugated
anti-Complement Component 5 (C5) (C-Term) antibodyC5 Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Complement Component 5 (C5) (AA 1-77) antibodyC5 Reactivity: Rat WB, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Complement Component 5 (C5) (AA 253-281), (N-Term) antibodyC5 Reactivity: Human WB, IHC (p), FACS Host: Rabbit Polyclonal unconjugated
anti-Complement Component 5 (C5) (AA 1550-1661) antibodyC5 Reactivity: Human WB, ELISA Host: Mouse Polyclonal unconjugated
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- Application Notes
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. - Comment
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Concentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- Preservative
- Thimerosal (Merthiolate), Sodium azide
- Precaution of Use
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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- Target
- C5 (Complement Component 5 (C5))
- Alternative Name
- Complement C5 (C5 Products)
- Synonyms
- C5 antibody, LOC565774 antibody, C5a antibody, C5b antibody, CPAMD4 antibody, Hc antibody, RGD1561905 antibody, He antibody, complement C5 antibody, complement component 5 antibody, hemolytic complement antibody, C5 antibody, c5 antibody, Hc antibody
- Background
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C5a is a protein fragment released from complement component C5. This gene is mapped to 9q33.2. The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to susceptibility to liver fibrosis and to rheumatoid arthritis.
Synonyms: Anaphylatoxin C5a analog antibody|Anaphylatoxin C5a antibody|C5 antibody|C5a antibody|Complement C5 antibody|Complement component C5 antibody|Complement component C5a antibody|CPAMD 4 antibody|CPAMD4 antibody - Gene ID
- 362119
- UniProt
- P08650
- Pathways
- Complement System, Carbohydrate Homeostasis
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