Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

alpha Adaptin antibody (AA 706-727)

Verified AP2A1 Reactivity: Human WB, ELISA Host: Goat Polyclonal unconjugated
Catalog No. ABIN5539681
  • Target See all alpha Adaptin (AP2A1) Antibodies
    alpha Adaptin (AP2A1) (Adaptor-Related Protein Complex 2, alpha 1 Subunit (AP2A1))
    Binding Specificity
    • 8
    • 8
    • 6
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 706-727
    Reactivity
    • 45
    • 16
    • 13
    • 5
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 35
    • 6
    • 4
    Goat
    Clonality
    • 38
    • 7
    Polyclonal
    Conjugate
    • 22
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This alpha Adaptin antibody is un-conjugated
    Application
    • 33
    • 22
    • 22
    • 9
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA
    Purpose
    AP2A1 (aa706-727)
    Sequence
    ELEPPAPESP MALLADPAPA AD
    Specificity
    This antibody is expected to recognize reported isoform 1 (NP_055018.2) only.
    Cross-Reactivity
    Cow, Human, Mouse, Pig, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Isotype
    IgG
  • Application Notes

    Western Blot: Approx 110 kDa band observed in Human Brain (Frontal cortex) lysates (calculated MW of 108 kDa according to NP_055018.2). Recommended concentration: 0.03-0.1 μg/mL. Primary incubation was 1 hour.

    Peptide ELISA: antibody detection limit dilution 1:128000.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Buser, Ritz, Moes, Tostado, Frank, Spiess, Mariani, Jenö, Boulay, Hutter: "Quantitative proteomics reveals reduction of endocytic machinery components in gliomas." in: EBioMedicine, Vol. 46, pp. 32-41, (2020) (PubMed).

  • Target
    alpha Adaptin (AP2A1) (Adaptor-Related Protein Complex 2, alpha 1 Subunit (AP2A1))
    Alternative Name
    AP2A1 (AP2A1 Products)
    Background
    AP2A1, adaptor-related protein complex 2, alpha 1 subunit, ADTAA, AP2-ALPHA, CLAPA1, 100 kDa coated vesicle protein A, AP-2 complex subunit alpha-1, adapter-related protein complex 2 alpha-1 subunit, adaptin, alpha A, adaptor protein complex AP-2 subunit
    Gene ID
    160, 11771, 308578
    NCBI Accession
    NP_055018
    Pathways
    Notch Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation
You are here:
Support