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Goat anti-Mouse IgG2b (Heavy Chain) Antibody (HRP)

ELISA, IHC, WB Polyclonal IgG HRP
Catalog No. ABIN376251
  • Target See all IgG2b products
    IgG2b
    Binding Specificity
    • 35
    • 16
    • 5
    • 3
    Heavy Chain
    Reactivity
    • 80
    • 24
    Mouse
    Host
    • 59
    • 25
    • 12
    • 7
    • 1
    Goat
    Clonality
    • 71
    • 19
    Polyclonal
    Conjugate
    • 17
    • 11
    • 10
    • 8
    • 7
    • 5
    • 5
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    HRP
    Application
    • 47
    • 45
    • 32
    • 30
    • 26
    • 21
    • 7
    • 6
    • 6
    • 5
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Specificity
    Reacts with the heavy chain of mouse IgG 2b as demonstrated by ELISA and flow cytometry.
    Characteristics
    Source: Pooled antisera from goats hyperimmunized with mouse IgG 2b paraproteins.
    To insure lot-to-lot consistency, each batch of product is tested by ELISA, PCFIA and/or flow cytometry for conformance to characteristics of a standard reference reagent.
    Purification
    Affinity chromatography on mouse IgG 3 covalently linked to agarose.
    Isotype
    IgG
  • Application Notes
    Working Dilution:
    1:4,000-1:8,000
    Representative data are included in this product insert.

    Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Handling Advice
    Avoid repeated freezing and thawing. Dilute only prior to immediate use
    Centrifuge product if not completely clear after standing at room temperature.
    Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
    Storage
    4 °C
  • Jin, Nesbitt, Yang, Chen, Horowitz, Jones, Vandergaast, Carey, Reiter, Russell, Kyratsous, Hooper, Hamilton, Ferreira, Deng, Straus, Baras, Hillyer, Luchsinger: "Seroprevalence of anti-SARS-CoV-2 antibodies in a cohort of New York City metro blood donors using multiple SARS-CoV-2 serological assays: Implications for controlling the epidemic and "Reopening"." in: PloS one, Vol. 16, Issue 4, pp. e0250319, (2021) (PubMed).

    Nesbitt, Jin, Hogan, Yang, Chen, Chan, Simon, Vargas, King, Huard, Bandy, Hillyer, Luchsinger: "Low Seroprevalence of SARS-CoV-2 in Rhode Island blood donors during may 2020 as determined using multiple serological assay formats." in: BMC infectious diseases, Vol. 21, Issue 1, pp. 871, (2021) (PubMed).

    Luchsinger, Ransegnola, Jin, Muecksch, Weisblum, Bao, George, Rodriguez, Tricoche, Schmidt, Gao, Jawahar, Pal, Schnall, Zhang, Strauss, Yazdanbakhsh, Hillyer, Bieniasz, Hatziioannou: "Serological Assays Estimate Highly Variable SARS-CoV-2 Neutralizing Antibody Activity in Recovered COVID19 Patients." in: Journal of clinical microbiology, (2020) (PubMed).

  • Target
    IgG2b
    Abstract
    IgG2b Products
    Target Type
    Antibody
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