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DDX5 antibody (AA 475-614)

Name: DDX5 antibody (AA 475-614)
Brand: antibodies-online
SKU: ABIN5542510
Price: 511.5 USD
Availability: InStock

DDX5 Reactivity: Human, Mouse, Monkey WB, ELISA, IHC, ICC, FACS Host: Mouse Monoclonal 4F1C12 unconjugated

Western blot analysis using DDX5 mouse mAb against HT-29 (1), Hela (2), NIH/3T3 (3), COS7 (4), SW620 (5), Jurkat (6), A431 (7), and MCF-7 (8) cell lysate.

Flow cytometric analysis of Hela cells using DDX5 mouse mAb (green) and negative control (red).

FACS

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using DDX5 mouse mAb with DAB staining.

IHC

Black line: Control Antigen (100 ng),Purple line: Antigen (10 ng), Blue line: Antigen (50 ng), Red line:Antigen (100 ng)

ELISA

Western blot analysis using DDX5 mAb against human DDX5 (AA: 475-614) recombinant protein.

Western blot analysis using DDX5 mAb against HEK293 (1) and DDX5 (AA: 475-614)-hIgGFc transfected HEK293 (2) cell lysate.

6 images

Catalog No. ABIN5542510

Datasheet as PDF

Target See all DDX5 Antibodies

DDX5 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5))
Binding Specificity
19

11

4

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
AA 475-614
Reactivity
67

46

41

6

6

6

6

5

4

3

1

1

1
Human, Mouse, Monkey
Host
61

6

1
Mouse
Compatible Secondaries
Clonality
53

15
Monoclonal
Conjugate
44

3

2

2

2

2

2

2

2

2

1

1

1

1

1
This DDX5 antibody is un-conjugated
Application
54

18

16

14

14

13

11

8

7

3

3

3

2

2

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Purification

purified

Immunogen

Purified recombinant fragment of human DDX5 (AA: 475-614) expressed in E. coli.

Clone

4F1C12

Isotype

IgG2a
anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5) (AA 1-614) antibody
DDX5 Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated

anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5) (C-Term) antibody
Verified DDX5 Reactivity: Human, Mouse WB, ELISA Host: Goat Polyclonal unconjugated

anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5) (AA 1-614) antibody
DDX5 Reactivity: Human WB, IF Host: Mouse Polyclonal unconjugated

anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5) (AA 1-290) antibody
DDX5 Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5) antibody
DDX5 Reactivity: Human WB, ELISA, IHC, IF, FACS Host: Rabbit Monoclonal 2C3 unconjugated Recombinant Antibody

anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5) (C-Term) antibody
DDX5 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5) (C-Term) antibody
DDX5 Reactivity: Human WB, IF, IHC (p), ICC Host: Rabbit Polyclonal unconjugated

anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5) (C-Term) antibody
DDX5 Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit WB Host: Rabbit Polyclonal unconjugated

anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5) (AA 1-210) antibody
DDX5 Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5) (pTyr593) antibody
DDX5 Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro) Host: Rabbit Polyclonal unconjugated

Application Notes

ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, ICC: , FCM: 1:200 - 1:400

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified antibody in PBS with 0.05 % sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C/-20 °C

Storage Comment

4°C, -20°C for long term storage
Target

DDX5 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5))

Alternative Name

DDX5 (DDX5 Products)

Synonyms

G17P1 antibody, HLR1 antibody, HUMP68 antibody, p68 antibody, 2600009A06Rik antibody, Hlr1 antibody, wu:fa56a07 antibody, wu:fb11e01 antibody, wu:fb16c10 antibody, wu:fb53b05 antibody, ddx5 antibody, DEAD-box helicase 5 antibody, DEAD (Asp-Glu-Ala-Asp) box polypeptide 5 antibody, DEAD (Asp-Glu-Ala-Asp) box helicase 5 antibody, DEAD-box helicase 5 L homeolog antibody, DDX5 antibody, Ddx5 antibody, ddx5 antibody, ddx5.L antibody

Background

Description: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified.,
Aliases: p68, HLR1, G17P1, HUMP68

Molecular Weight

69.1 kDa

Gene ID

1655

HGNC

1655

Pathways

Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Regulation of Muscle Cell Differentiation, Positive Regulation of Response to DNA Damage Stimulus