Ubiquitin antibody
-
- Target See all Ubiquitin Antibodies
- Ubiquitin
-
Reactivity
- Human, Cow, Chicken, Drosophila melanogaster, C. elegans
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- This Ubiquitin antibody is un-conjugated
-
Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
- Purification
- affinity purified antibody
- Clone
- Ubi-1-AP
- Isotype
- IgG1
- Top Product
- Discover our top product Ubiquitin Primary Antibody
-
anti-Ubiquitin (Ubiquitin) antibody
Ubiquitin Reactivity: Cow WB, IHC, IF, ICC, IP Host: Rabbit Polyclonal unconjugated
anti-Ubiquitin (Ubiquitin) (AA 1-229) antibodyUbiquitin Reactivity: Human, Rat, Mouse, Cow, Pig, Rabbit, Dog, Horse WB, IHC, ICC, IP Host: Rabbit Polyclonal unconjugated
anti-Ubiquitin (Ubiquitin) (AA 1-229) antibodyUbiquitin Reactivity: Human, Rat WB, IHC, ICC, IP Host: Mouse Monoclonal D2 unconjugated
anti-Ubiquitin (Ubiquitin) (acLys27) antibodyUbiquitin Reactivity: Human, Rat, Mouse WB, ELISA, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Ubiquitin (Ubiquitin) antibody (HRP)Ubiquitin Reactivity: Cow WB, IHC, IF, ICC, IP Host: Rabbit Polyclonal HRP
anti-Ubiquitin (Ubiquitin) antibody (FITC)Ubiquitin Reactivity: Cow WB, IHC, IF, ICC, IP Host: Rabbit Polyclonal FITC
anti-Ubiquitin (Ubiquitin) antibody (Alkaline Phosphatase (AP))Ubiquitin Reactivity: Cow WB, IHC, IF, ICC, IP Host: Rabbit Polyclonal Alkaline Phosphatase (AP)
anti-Ubiquitin (Ubiquitin) antibody (Biotin)Ubiquitin Reactivity: Cow WB, IHC, IF, ICC, IP Host: Rabbit Polyclonal Biotin
anti-Ubiquitin (Ubiquitin) antibody (PE)Ubiquitin Reactivity: Cow WB, IHC, IF, ICC, IP Host: Rabbit Polyclonal PE
anti-Ubiquitin (Ubiquitin) antibody (APC)Ubiquitin Reactivity: Cow WB, IHC, IF, ICC, IP Host: Rabbit Polyclonal APC
-
- Application Notes
- MCA-Ubi-1 is excellent for the detection of ubiquitinated inclusions seen in human neurodegenerative diseases such as the neurofibrillary tangles of Alzheimer's disease. We recommend a starting dilution of 1:1,000 for this purposes, using ABC or other enzymatic amplification procedures. For immunofluorescence try a dilution of 1:500.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- The antibody is supplied purified form at a concentration of 1 mg/mL. Purification is accomplished using a Protein A affinity column, and the purified antibody is in a phosphate buffered saline solution. We added 11 mM sodium azide preservative .
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 4 °C short term or -24 °C long term.
-
- Target
- Ubiquitin
- Alternative Name
- Ubiquitin (Ubiquitin Products)
- Synonyms
- CG11624 antibody, DmUb antibody, DmUbi-p63E antibody, Dmel\\CG11624 antibody, Ub antibody, Ubi-P63e antibody, Ubi-p antibody, Ubi63E antibody, Ubi63p antibody, Ubip antibody, Ubiq antibody, Ubp antibody, ubi antibody, ubi-p63E antibody, ubi63E antibody, CG18282 antibody, CG32744 antibody, CR32744 antibody, DmUbi-p5E antibody, Dmel\\CG32744 antibody, ubiquitin antibody, si:dz180g5.5 antibody, si:busm1-180g5.5 antibody, LOC100194618 antibody, UBIQ antibody, Ubiquitin antibody, LOC100282068 antibody, Ubiquitin-63E antibody, Ubiquitin-5E antibody, transmembrane and ubiquitin-like domain containing 2 antibody, polyubiquitin antibody, Putative ubiquitin family protein antibody, ubiquitin antibody, ubiquitin B antibody, UBiQuitin antibody, Ubiquitin antibody, Ubi-p63E antibody, Ubi-p5E antibody, tmub2 antibody, LOC100194618 antibody, pco110026 antibody, LOC100135798 antibody, UBB antibody, ubq-1 antibody, LOC100190766 antibody
- Background
- Ubiquitin is a highly conserved globular 76 amino acid protein of about 8.5 kDa molecular weight. It has a important role in the targeting of proteins for proteolytic degradation. Proteins to be degraded are covalantly coupled to the C-terminus of ubiquitin by means of ubiquitin ligases. The ubiquitin itself is frequently also ubiquitinated, producing a polyubiquitin chain. The polyubquitinated complex is then recognized by a complex of degradative enzymes which together form the proteosome. Interestingly, ubiquitin also becomes covalently bonded to many types of pathological inclusions seen in serious human disease states which appear to be resistant to normal degradation, so that ubiquitin antibodies are very useful for studies of these inclusions. For example the neurofibrillary tangles and paired helical filaments diagnostic of Alzheimer's disease, the Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease are all heavily ubiquitinated and can all be readily visualized with ubiquitin antibodies of appropriate specificity.
- Pathways
- Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway
-
