NEK2 antibody (AA 1-100)
(3 references)-
- Target See all NEK2 Antibodies
- NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))
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Binding Specificity
- AA 1-100
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This NEK2 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Cross-Reactivity
- Human
- Predicted Reactivity
- Mouse,Rat,Dog,Cow,Pig,Horse,Rabbit
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic peptide derived from human NEK2
- Isotype
- IgG
- Top Product
- Discover our top product NEK2 Primary Antibody
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anti-NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (AA 148-397) antibody
NEK2 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (AA 331-445) antibodyNEK2 Reactivity: Human WB, ELISA, IP, IHC (p) Host: Mouse Monoclonal 2F6 unconjugated
anti-NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (AA 331-445) antibodyNEK2 Reactivity: Human WB, ELISA, IF Host: Mouse Monoclonal 2F9 unconjugated
anti-NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (AA 1-445) antibodyNEK2 Reactivity: Human WB, IP Host: Rabbit Polyclonal unconjugated
anti-NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (AA 1-445) antibodyNEK2 Reactivity: Human WB, IF Host: Mouse Polyclonal unconjugated
anti-NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (AA 396-426) antibodyNEK2 Reactivity: Human WB Host: Rabbit Polyclonal RB2959 unconjugated
anti-NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (AA 331-445) antibodyNEK2 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 1C8 unconjugated
anti-NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (AA 287-299) antibodyNEK2 Reactivity: Human WB, ELISA Host: Rabbit Polyclonal unconjugated
anti-NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (AA 282-295) antibodyNEK2 Reactivity: Human WB Host: Sheep Monoclonal unconjugated
anti-NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (AA 125-393) antibodyNEK2 Reactivity: Human WB, IHC, ICC Host: Rabbit Polyclonal unconjugated
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- Application Notes
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WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Expiry Date
- 12 months
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: "Overexpression of NIMA-related kinase 2 is associated with progression and poor prognosis of prostate cancer." in: BMC urology, Vol. 15, pp. 90, (2015) (PubMed).
: "Examining Nek2 as a better proliferation marker in non-small cell lung cancer prognosis." in: Tumour biology, Vol. 35, Issue 7, pp. 7155-62, (2014) (PubMed).
: "Aberrant expression of NEK2 and its clinical significance in non-small cell lung cancer." in: Oncology letters, Vol. 8, Issue 4, pp. 1470-1476, (2014) (PubMed).
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: "Overexpression of NIMA-related kinase 2 is associated with progression and poor prognosis of prostate cancer." in: BMC urology, Vol. 15, pp. 90, (2015) (PubMed).
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- Target
- NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))
- Alternative Name
- NEK2 (NEK2 Products)
- Synonyms
- AA617254 antibody, C77054 antibody, HsPK21 antibody, NEK2A antibody, NLK1 antibody, ATNEK2 antibody, NIMA-RELATED KINASE2 antibody, NIMA-related kinase 2 antibody, T9J14.24 antibody, T9J14_24 antibody, NIMA related kinase 2 antibody, NIMA (never in mitosis gene a)-related expressed kinase 2 antibody, NIMA-related kinase 2 antibody, NEK2 antibody, Nek2 antibody
- Background
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Synonyms: NLK1, RP67, NEK2A, HsPK21, PPP1R111, Serine/threonine-protein kinase Nek2, HSPK 21, Never in mitosis A-related kinase 2, NimA-related protein kinase 2, NimA-like protein kinase 1, NEK2
Background: Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP25 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC8. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC2 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC, SGOL1, NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the nucleolus, does not.
- Gene ID
- 4751
- UniProt
- P51955
- Pathways
- M Phase
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