NOTCH4 Protein (AA 1411-1964) (rho-1D4 tag)
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- Target See all NOTCH4 Proteins
- NOTCH4 (Notch 4 (NOTCH4))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1411-1964
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Origin
- Mouse
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Source
- Insect Cells
- Purification tag / Conjugate
- This NOTCH4 protein is labelled with rho-1D4 tag.
- Application
- SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys), ELISA
- Sequence
- MAAVGALEPL LPGPLLAAHP QAGTRPSANQ LPWPILCSPV VGVLLLALGA LLVLQLIRRR RREHGALWLP PGFIRRPQTQ QAPHRRRPPL GEDNIGLKAL KPEAEVDEDG VAMCSGPEEG EAEETASASR CQLWPLNSGC GELPQAAMLT PPQECESEVL DVDTCGPDGV TPLMSAVFCG GVQSTTGASP QRLGLGNLEP WEPLLDRGAC PQAHTVGTGE TPLHLAARFS RPTAARRLLE AGANPNQPDR AGRTPLHTAV AADAREVCQL LLASRQTTVD ARTEDGTTPL MLAARLAVED LVEELIAARA DVGARDKRGK TALHWAAAVN NARAARSLLQ AGADKDAQDS REQTPLFLAA REGAVEVAQL LLELGAARGL RDQAGLAPGD VARQRSHWDL LTLLEGAGPT TQEARAHART TPGGGAAPRC RTLSAGARPR GGGACLQART WSVDLGARGG KVYARCRSRS GSCGGPTTRG RRFSAGSRGR RGARASQDDW PRDWVALEAC GSACSAPIPP PSLTPSPERG SPQVAWGLPV HQEIPLNSVV RNLNGSSGTE TSQVAPA
- Specificity
- C-terminal Rho-tag
- Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Notch4 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This made-to-order protein has already been successfully produced. Please let us know if you are interested in purchasing a smaller amount of this protein. We will check our stock and make you a customized quote in case we can provide this protein in a smaller amount..
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin-free.
- Grade
- Crystallography grade
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Notch 4 (NOTCH4) (AA 1042-1441) protein (His tag)
NOTCH4 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Notch 4 (NOTCH4) (AA 1469-2003) protein (His tag)Crystallography grade NOTCH4 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, Crys, ELISA
Notch 4 (NOTCH4) (AA 1465-1964) protein (His tag)Crystallography grade NOTCH4 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, Crys, ELISA
Notch 4 (NOTCH4) (AA 1467-2003) protein (rho-1D4 tag)Crystallography grade NOTCH4 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, Crys, ELISA
Notch 4 (NOTCH4) (AA 1432-2003) protein (rho-1D4 tag)Crystallography grade NOTCH4 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, Crys, ELISA
Notch 4 (NOTCH4) (AA 1463-1964) protein (rho-1D4 tag)Crystallography grade NOTCH4 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, Crys, ELISA
Notch 4 (NOTCH4) (AA 1428-1964) protein (rho-1D4 tag)Crystallography grade NOTCH4 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, Crys, ELISA
Notch 4 (NOTCH4) (AA 24-1447) protein (His tag)Crystallography grade NOTCH4 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, Crys, ELISA
Notch 4 (NOTCH4) (AA 21-1443) protein (His tag)Crystallography grade NOTCH4 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, Crys, ELISA
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 20 mM Tris, pH 8.0; 300 mM NaCl; 2 mM DTT, 2 mM EDTA, 0.1% Triton X-100
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- NOTCH4 (Notch 4 (NOTCH4))
- Alternative Name
- Notch4 (NOTCH4 Products)
- Background
- Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). May regulate branching morphogenesis in the developing vascular system. {ECO:0000250, ECO:0000269|PubMed:11344305}.
- Molecular Weight
- 59.5 kDa Including tag.
- UniProt
- P31695
- Pathways
- Notch Signaling
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