DSCAM antibody (Internal Region)
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- Target See all DSCAM Antibodies
- DSCAM (Down Syndrome Cell Adhesion Molecule (DSCAM))
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Binding Specificity
- Internal Region
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This DSCAM antibody is un-conjugated
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Application
- ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)
- Purpose
- DSCAM
- Sequence
- DSWDSAQRTK DVSPQ
- Specificity
- This antibody is expected to recognise both reported isoforms (NP_001380.2 and NP_996770.1).
- Cross-Reactivity
- Dog, Human, Mouse, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence C-DSWDSAQRTKDVSPQ, from the internal region of the protein sequence according to NP_001380.2, NP_996770.1.
- Isotype
- IgG
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anti-Down Syndrome Cell Adhesion Molecule (DSCAM) (AA 1711-1867) antibody
DSCAM Reactivity: Human ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-Down Syndrome Cell Adhesion Molecule (DSCAM) (AA 301-400) antibodyDSCAM Reactivity: Mouse, Rat ELISA, IF (cc), IF (p), IHC (p), ICC, IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Down Syndrome Cell Adhesion Molecule (DSCAM) (AA 1548-1597) antibodyDSCAM Reactivity: Human, Mouse, Rat, Dog, Horse, Rabbit, Monkey, Bat WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Down Syndrome Cell Adhesion Molecule (DSCAM) (AA 184-271) antibodyDSCAM Reactivity: Human ELISA, WB Host: Mouse Polyclonal unconjugated
anti-Down Syndrome Cell Adhesion Molecule (DSCAM) (Middle Region) antibodyDSCAM Reactivity: Human, Mouse, Rat, Dog, Horse, Cow, Rabbit, Guinea Pig, Pig WB, IP Host: Rabbit Polyclonal unconjugated
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- Application Notes
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Western Blot: Approx 200-220 kDa band observed in HepG2 cell lysates (calculated MW of 174 kDa according to NP_996770.1 ). This molecular weight is routinely observed by other sources. Recommended concentration: 2-3 μg/mL. Primary incubation 1 hour at room
Peptide ELISA: antibody detection limit dilution 1:16000.
- Comment
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Immunofluorescence: Strong expression of the protein seen in the nuclei of MCF7 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- DSCAM (Down Syndrome Cell Adhesion Molecule (DSCAM))
- Alternative Name
- DSCAM (DSCAM Products)
- Synonyms
- CHD2-42 antibody, CHD2-52 antibody, Dscam antibody, DSCAM antibody, chd2-42 antibody, chd2-52 antibody, dscam antibody, GB15141 antibody, 4932410A21Rik antibody, 43Bc antibody, CG17800 antibody, CT39257 antibody, DScam antibody, DmDscam antibody, Dm_2R:13612 antibody, Dmel\\CG17800 antibody, Dscam-hv antibody, Dscam1 antibody, FBgn0033159 antibody, Neu1 antibody, dScam antibody, l(2)05518 antibody, l(2)43Bc antibody, p270 antibody, DS cell adhesion molecule antibody, Down syndrome cell adhesion molecule antibody, Down syndrome cell adhesion molecule a antibody, Down syndrome cell adhesion molecule 1 antibody, Down syndrome cell adhesion molecule-like protein Dscam2 antibody, down syndrome cell adhesion molecule antibody, Down syndrome cell adhesion molecule L homeolog antibody, DSCAM antibody, Dscam antibody, dscam antibody, dscama antibody, Dscam1 antibody, LOC5573626 antibody, CpipJ_CPIJ006173 antibody, dscam.L antibody
- Background
- DSCAM, Down syndrome cell adhesion molecule , CHD2-42, CHD2-52 , human CHD2-52 down syndrome cell adhesion molecule
- Gene ID
- 1826, 13508, 171119
- NCBI Accession
- NP_001380, NP_001258463
- Pathways
- Regulation of Cell Size
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