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OXT antibody (C-Term)

Verified OXT Reactivity: Mouse ELISA, IF, FACS Host: Goat Polyclonal unconjugated
Catalog No. ABIN570836
  • Target See all OXT Antibodies
    OXT (Oxytocin, Prepropeptide (OXT))
    Binding Specificity
    • 6
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 27
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Host
    • 26
    • 1
    • 1
    • 1
    Goat
    Clonality
    • 28
    • 1
    Polyclonal
    Conjugate
    • 17
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This OXT antibody is un-conjugated
    Application
    • 15
    • 13
    • 9
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
    Purpose
    OXT (mouse)
    Sequence
    DGCRTDPACD PES
    Cross-Reactivity
    Mouse, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    DGCRTDPACDPES
    Isotype
    IgG
    Top Product
    Discover our top product OXT Primary Antibody
  • Application Notes

    Western Blot: Preliminary experiments gave an approx 22 kDa band in Mouse Liver after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for this band, given the calculated size of 12.9 kDa according to NP

    Peptide ELISA: antibody detection limit dilution 1:16000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm and membranes of NIH3T3 cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of NIH3T3 cells. Recommended concen

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • McMahon, Chang, Jackson: "Multiple cytosolic calcium buffers in posterior pituitary nerve terminals." in: The Journal of general physiology, Vol. 147, Issue 3, pp. 243-54, (2016) (PubMed).

  • Target
    OXT (Oxytocin, Prepropeptide (OXT))
    Alternative Name
    Oxt (OXT Products)
    Synonyms
    OT antibody, OT-NPI antibody, OXT-NPI antibody, Oxy antibody, OTNPI antibody, NEURL1 antibody, RNF67 antibody, bA416N2.1 antibody, neu antibody, neu-1 antibody, Ocytocin antibody, Oxytocin antibody, oxytocin/neurophysin I prepropeptide antibody, oxytocin antibody, oxytocin/neurophysin 1 prepropeptide antibody, neuralized E3 ubiquitin protein ligase 1 antibody, vasopressin-neurophysin 2 antibody, OXT antibody, Oxt antibody, NEURL1 antibody, LOC100066842 antibody
    Background
    Oxt, oxytocin, OT, Oxy, OTTMUSP00000016535
    Gene ID
    18429, 25504
    NCBI Accession
    NP_035155
    Pathways
    Myometrial Relaxation and Contraction, Feeding Behaviour
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