For best experience we recommend to activate Javascript in your browser.

PAK1 antibody (Center)

Name: PAK1 antibody (Center)
Brand: antibodies-online
SKU: ABIN2855705
Price: 609.18 USD
Availability: InStock

PAK1 Reactivity: Human, Rat, Mouse WB, IF, IHC (p), ICC Host: Rabbit Polyclonal unconjugated

WB Image PAK1 antibody detects PAK1 protein by western blot analysis.

WB Image Sample (30 ug of whole cell lysate) A: A431 B: HeLa 7.

ICC/IF Image PAK1 antibody detects PAK1 protein at cytoplasm by immunofluorescent analysis.

IHC-P Image PAK1 antibody detects PAK1 protein on human hepatoma by immunohistochemical analysis.

IHC

6 images

(1 reference)

Catalog No. ABIN2855705

Datasheet as PDF

Target See all PAK1 Antibodies

PAK1 (P21-Activated Kinase 1 (PAK1))
Binding Specificity
34

17

15

13

10

10

9

9

8

7

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Center
Reactivity
172

94

82

7

5

4

4

3

3

2

1

1
Human, Rat, Mouse
Host
165

4

1

1
Rabbit
Compatible Secondaries
Clonality
160

12
Polyclonal
Conjugate
89

10

7

7

6

6

6

6

6

6

4

4

3

3

3

3

3
This PAK1 antibody is un-conjugated
Application
115

66

45

39

39

33

30

14

11

10

9

6

4

3

3

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Cross-Reactivity

Cow (Bovine)

Cross-Reactivity (Details)

Bovine (100 %)

Characteristics

Rabbit Polyclonal antibody to PAK1 (p21 protein (Cdc42/Rac)-activated kinase 1)
PAK1 antibody

Purification

Purified by antigen-affinity chromatography.

Immunogen

Recombinant protein encompassing a sequence within the center region of human PAK1. The exact sequence is proprietary.

Isotype

IgG
anti-P21-Activated Kinase 1 (PAK1) (AA 191-280) antibody
PAK1 Reactivity: Human WB, ELISA, IF, IHC (p), IP Host: Mouse Monoclonal 4D1 unconjugated

anti-P21-Activated Kinase 1 (PAK1) (AA 252-501) antibody
PAK1 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-P21-Activated Kinase 1 (PAK1) (pThr212) antibody
PAK1 Reactivity: Human, Rat, Mouse WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-P21-Activated Kinase 1 (PAK1) (AA 1-240) antibody
PAK1 Reactivity: Human WB Host: Rabbit Polyclonal unconjugated

anti-P21-Activated Kinase 1 (PAK1) (pThr423) antibody
PAK1 Reactivity: Human WB, IF Host: Rabbit Polyclonal RB11381 unconjugated

anti-P21-Activated Kinase 1 (PAK1) (AA 401-430) antibody
PAK1 Reactivity: Human WB, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated

anti-P21-Activated Kinase 1 (PAK1) (Ser204) antibody
PAK1 Reactivity: Human WB, IHC, ELISA Host: Rabbit Polyclonal unconjugated

anti-P21-Activated Kinase 1 (PAK1) (N-Term) antibody
PAK1 Reactivity: Human, Rat, Mouse WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-P21-Activated Kinase 1 (PAK1) (pThr212) antibody
PAK1 Reactivity: Human WB, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated

anti-P21-Activated Kinase 1 (PAK1) (AA 401-430) antibody
PAK1 Reactivity: Human WB, IF, IHC (p) Host: Rabbit Polyclonal RB11382 unconjugated

Application Notes

Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:10000*

Comment

Positive Control: A431 , HeLa , BCL-1 , PC-12 , SH-SY5Y

Restrictions

For Research Use only
Format

Liquid

Concentration

0.48 mg/mL

Buffer

0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative

Thimerosal (Merthiolate)

Precaution of Use

This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Cai, Zhang, Zhang, Chen, Hu: "Effect of Th9/IL-9 on the growth of gastric cancer in nude mice." in: OncoTargets and therapy, Vol. 12, pp. 2225-2234, (2019) (PubMed).
Target

PAK1 (P21-Activated Kinase 1 (PAK1))

Alternative Name

PAK1 (PAK1 Products)

Synonyms

X-PAK1 antibody, XPak1 antibody, pak antibody, pak-1 antibody, pakalpha antibody, PAKalpha antibody, AW045634 antibody, PAK-1 antibody, Paka antibody, p21 protein (Cdc42/Rac)-activated kinase 1 S homeolog antibody, p21 (RAC1) activated kinase 1 antibody, p21 protein (Cdc42/Rac)-activated kinase 1 antibody, Serine/threonine-protein kinase pak-1 antibody, pak1.S antibody, PAK1 antibody, Pak1 antibody, pak-1 antibody

Background

PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology. Alternativelt spliced transcript variants encoding different isoforms have been found for this gene.

Cellular Localization: Cytoplasm , Cell junction , focal adhesion

Molecular Weight

61 kDa

Gene ID

5058

Pathways

MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Embryonic Body Morphogenesis