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PTK2B antibody (pTyr402)

Name: PTK2B antibody (pTyr402)
SKU: ABIN745763
Price: 384.62 USD
Availability: InStock

PTK2B Reactivity: Mouse, Rat WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti phospho-Pyk2/PTK2B(Tyr402) Polyclonal Antibody, Unconjugated (ABIN745763) at 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC

Paraformaldehyde-fixed, paraffin embedded mouse brain, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Pyk2 (Tyr402) Polyclonal Antibody (bs-3400R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.

IHC (p)

Formalin-fixed and paraffin embedded rat spleen labeled with Rabbit Anti phospho-Pyk2/PTK2B(Tyr402) Polyclonal Antibody, Unconjugated (ABIN745763) at 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC

Paraformaldehyde-fixed, paraffin embedded rat brain, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Pyk2 (Tyr402) Polyclonal Antibody (bs-3400R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.

IHC (p)

4 images

(1 reference)

Catalog No. ABIN745763

Datasheet as PDF

Target See all PTK2B Antibodies

PTK2B (PTK2B Protein tyrosine Kinase 2 beta (PTK2B))
Binding Specificity
24

20

17

15

10

7

6

6

6

5

4

4

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pTyr402
Reactivity
114

114

95

8

7

7

6

5

5

5

1

1

1
Mouse, Rat
Host
145

13

3
Rabbit
Compatible Secondaries
Clonality
140

21
Polyclonal
Conjugate
75

11

8

7

7

7

7

7

7

6

3

3

3

3

3

2

2
This PTK2B antibody is un-conjugated
Application
123

58

44

39

38

25

13

13

8

4

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity

Mouse, Rat

Predicted Reactivity

Human,Dog,Cow,Pig,Horse,Rabbit

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic phosphopeptide derived from human Pyk2/PTK2B around the phosphorylation site of Tyr402

Isotype

IgG
anti-PTK2B Protein tyrosine Kinase 2 beta (PTK2B) (AA 1-1009) antibody
PTK2B Reactivity: Human WB, IF, PLA Host: Rabbit Polyclonal unconjugated

anti-PTK2B Protein tyrosine Kinase 2 beta (PTK2B) (AA 682-871) antibody
PTK2B Reactivity: Human WB, ELISA, IP Host: Mouse Monoclonal 1F9 unconjugated

anti-PTK2B Protein tyrosine Kinase 2 beta (PTK2B) (pTyr402) antibody
PTK2B Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-PTK2B Protein tyrosine Kinase 2 beta (PTK2B) (AA 805-838), (C-Term) antibody
PTK2B Reactivity: Human WB, IHC (p), IF Host: Rabbit Polyclonal RB50594 unconjugated

anti-PTK2B Protein tyrosine Kinase 2 beta (PTK2B) antibody
PTK2B Reactivity: Human, Mouse, Rat WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-PTK2B Protein tyrosine Kinase 2 beta (PTK2B) (AA 815-997) antibody
PTK2B Reactivity: Human WB, ELISA, IHC Host: Mouse Monoclonal 5E2D5 unconjugated

anti-PTK2B Protein tyrosine Kinase 2 beta (PTK2B) (AA 682-871) antibody
PTK2B Reactivity: Human WB, ELISA Host: Mouse Monoclonal X1 unconjugated

anti-PTK2B Protein tyrosine Kinase 2 beta (PTK2B) (AA 812-861) antibody
PTK2B Reactivity: Human, Mouse, Rat, Dog, Rabbit, Pig, Horse, Monkey, Guinea Pig, Cow, Bat WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-PTK2B Protein tyrosine Kinase 2 beta (PTK2B) (Tyr579) antibody
PTK2B Reactivity: Human WB, ELISA Host: Rabbit Polyclonal unconjugated

anti-PTK2B Protein tyrosine Kinase 2 beta (PTK2B) (AA 707-875) antibody
PTK2B Reactivity: Human ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

Application Notes

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Cao, Zheng, Wang, Wang, Li, Li, Lai, Wang, Qin: "Src blockage by siRNA inhibits VEGF-induced vascular hyperpemeability and osteoclast activity - an in vitro mechanism study for preventing destructive repair of osteonecrosis." in: Bone, Vol. 74C, pp. 58-68, (2015) (PubMed).
Target

PTK2B (PTK2B Protein tyrosine Kinase 2 beta (PTK2B))

Alternative Name

Pyk2 (PTK2B Products)

Synonyms

CADTK antibody, CAKB antibody, FADK2 antibody, FAK2 antibody, PKB antibody, PTK antibody, PYK2 antibody, RAFTK antibody, CAKbeta antibody, E430023O05Rik antibody, Raftk antibody, Pyk2 antibody, fak1b antibody, ptk2.2 antibody, ptk2b antibody, si:ch1073-355e8.1 antibody, protein tyrosine kinase 2 beta antibody, PTK2 protein tyrosine kinase 2 beta antibody, protein tyrosine kinase 2aa antibody, PTK2B antibody, Ptk2b antibody, ptk2aa antibody

Background

Synonyms: Protein-tyrosine kinase 2-beta, Calcium-dependent tyrosine kinase, CADTK, Calcium-regulated non-receptor proline-rich tyrosine kinase, Cell adhesion kinase beta, CAK-beta, CAKB, Focal adhesion kinase 2, FADK 2, Proline-rich tyrosine kinase 2, Related adhesion focal tyrosine kinase, RAFTK, PTK2B, FAK2, PYK2
Background: Non-receptor protein-tyrosine kinase that regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading and bone remodeling. Plays a role in the regulation of the humoral immune response, and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. Required for normal macrophage polarization and migration towards sites of inflammation. Regulates cytoskeleton rearrangement and cell spreading in T-cells, and contributes to the regulation of T-cell responses. Promotes osteoclastic bone resorption, this requires both PTK2B/PYK2 and SRC. May inhibit differentiation and activity of osteoprogenitor cells. Functions in signaling downstream of integrin and collagen receptors, immune receptors, G-protein coupled receptors (GPCR), cytokine, chemokine and growth factor receptors, and mediates responses to cellular stress. Forms multisubunit signaling complexes with SRC and SRC family members upon activation, this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Regulates production of the cellular messenger cGMP. Promotes activation of the MAP kinase signaling cascade, including activation of MAPK1/ERK2, MAPK3/ERK1 and MAPK8/JNK1. Promotes activation of Rho family GTPases, such as RHOA and RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Acts as a scaffold, binding to both PDPK1 and SRC, thereby allowing SRC to phosphorylate PDPK1 at 'Tyr-9, 'Tyr-373', and 'Tyr-376'. Promotes phosphorylation of NMDA receptors by SRC family members, and thereby contributes to the regulation of NMDA receptor ion channel activity and intracellular Ca(2+) levels.

Gene ID

2185

UniProt

Q14289

Pathways

EGFR Signaling Pathway, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Cell-Cell Junction Organization, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGF Signaling