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EIF4A2 antibody (C-Term)

Name: EIF4A2 antibody (C-Term)
SKU: ABIN653137
Price: 429 USD
Availability: InStock

EIF4A2 Reactivity: Human WB, IHC (p), FACS Host: Rabbit Polyclonal RB23766 unconjugated

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with EIF4A2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

IHC (p)

EIF4A2 Antibody (C-term) (ABIN653137 and ABIN2842713) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).

FACS

Western blot analysis of EIF4A2 Antibody (C-term) (ABIN653137 and ABIN2842713) in K562 cell line lysates (35 μg/lane).

Western blot analysis of EIF4A2 (arrow) using rabbit polyclonal EIF4A2 Antibody (C-term) (ABIN653137 and ABIN2842713).

Anti-EIF4A2 Antibody (C-term) at 1:4000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 μg per lane.

5 images

Catalog No. ABIN653137

Datasheet as PDF

Target See all EIF4A2 Antibodies

EIF4A2 (Eukaryotic Translation Initiation Factor 4A2 (EIF4A2))
Binding Specificity
15

7

7

5

4

4

2

1

1

1

1

1

1
AA 333-360, C-Term
Reactivity
48

35

21

4

3

3

2

2

2

2

1

1

1
Human
Host
61

2
Rabbit
Compatible Secondaries
Clonality
62

1
Polyclonal
Conjugate
25

6

6

5

2

2

2

2

2

2

2

2

1

1

1

1

1
This EIF4A2 antibody is un-conjugated
Application
35

29

27

15

13

13

10

4

3

2

2

1

1

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Predicted Reactivity

B, Pr, M, Rb, C, Rat

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen

This EIF4A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 333-360 amino acids from the C-terminal region of human EIF4A2.

Clone

RB23766

Isotype

Ig Fraction
anti-Eukaryotic Translation Initiation Factor 4A2 (EIF4A2) (AA 1-270) antibody
EIF4A2 Reactivity: Human WB Host: Rabbit Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 4A2 (EIF4A2) (AA 1-407) antibody
EIF4A2 Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 4A2 (EIF4A2) antibody
EIF4A2 Reactivity: Human, Rat, Mouse IHC Host: Rabbit Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 4A2 (EIF4A2) antibody
EIF4A2 Reactivity: Human WB, IHC (p), IF, ICC, IHC (wm) Host: Rabbit Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 4A2 (EIF4A2) (AA 5-31), (N-Term) antibody
EIF4A2 Reactivity: Human, Rat, Mouse WB, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 4A2 (EIF4A2) antibody
EIF4A2 Reactivity: Human, Rat, Mouse WB, IHC, IF, IP, RIP Host: Rabbit Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 4A2 (EIF4A2) (AA 339-369), (C-Term) antibody
EIF4A2 Reactivity: Human WB, IHC (p), FACS, EIA Host: Rabbit Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 4A2 (EIF4A2) (AA 1-100) antibody
EIF4A2 Reactivity: Human WB, ELISA Host: Mouse Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 4A2 (EIF4A2) antibody
EIF4A2 Reactivity: Human, Rat, Mouse ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 4A2 (EIF4A2) (AA 1-50) antibody
EIF4A2 Reactivity: Human, Rat, Mouse, Pig, Cow WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated

Application Notes

WB: 1:1000. WB: 1:1000. WB: 1:4000. IHC-P: 1:50~100. FC: 1:10~50

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date

6 months
Target

EIF4A2 (Eukaryotic Translation Initiation Factor 4A2 (EIF4A2))

Alternative Name

EIF4A2 (EIF4A2 Products)

Synonyms

BM-010 antibody, DDX2B antibody, EIF4A antibody, EIF4F antibody, eIF-4A-II antibody, eIF4A-II antibody, 4833432N07Rik antibody, Ddx2b antibody, Eif4 antibody, ddx2b antibody, eif4aii antibody, eif4f antibody, xeif4aii antibody, EIF4A2 antibody, EIF4AII antibody, wu:fd50g11 antibody, zgc:63783 antibody, eukaryotic translation initiation factor 4A2 antibody, eukaryotic translation initiation factor 4A2 L homeolog antibody, microRNA 1248 antibody, eukaryotic translation initiation factor 4A, isoform 2 antibody, Eif4a2 antibody, EIF4A2 antibody, eif4a2.L antibody, eif4a2 antibody, MIR1248 antibody

Background

Eukaryotic initiation factor 4A plays an important role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins. Two highly homologous forms of functional EIF4A genes, Eif4a1 and Eif4a2, have been isolated in mice, yeast cells also possess 2 EIF4A genes, TIF1 and TIF2. The murine Eif4a and yeast TIF genes appear to belong to a DEAD-box gene family, whose members exhibit extensive amino acid similarity and contain the asp-glu-ala-asp (DEAD) sequence. DEAD-box genes have been identified in species ranging from E-coli to humans. Their function appears to be related to transcriptional/translational regulation (referenced from OMIM).

Molecular Weight

46402

Gene ID

1974

NCBI Accession

NP_001958

UniProt

Q14240