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Cortactin antibody (N-Term)

Name: Cortactin antibody (N-Term)
Brand: antibodies-online
SKU: ABIN2854674
Price: 609.18 USD
Availability: InStock
Rating: 5 (1 reviews)

KO Validated CTTN Reactivity: Human WB, IF, IHC (p), ICC Host: Rabbit Polyclonal unconjugated

Multiplex Immunohistochemistry validation image for anti-Cortactin (CTTN) (N-Term) antibody (ABIN2854674)

mIHC

WB Image Sample (30 ug of whole cell lysate) A: PC-12 7.

WB Image Sample (30 ug of whole cell lysate) A: Hep G2 , 7.

IHC-P Image Immunohistochemical analysis of paraffin-embedded human breast cancer, using Cortactin, antibody at 1:250 dilution.

IHC

ICC/IF Image Cortactin antibody [N1], N-term detects Cortactin protein at cell membrane and cytoplasm by immunofluorescent analysis.

WB Image Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7.

7 images

(1 validation)

Catalog No. ABIN2854674

Datasheet as PDF

Target See all Cortactin (CTTN) Antibodies

Cortactin (CTTN)
Binding Specificity
15

13

12

7

7

7

6

6

4

4

3

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
N-Term
Reactivity
122

60

43

5

4

2

2

2

2

2

2

1

1

1
Human
Host
122

6

1
Rabbit
Compatible Secondaries
Clonality
113

16
Polyclonal
Conjugate
72

8

5

5

5

5

5

5

4

4

3

3

1

1

1

1

1
This Cortactin antibody is un-conjugated
Application
94

52

51

15

14

14

13

11

9

6

4

3

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Cross-Reactivity

Human, Mouse, Rat

Characteristics

Rabbit Polyclonal antibody to Cortactin (src substrate cortactin)
Cortactin antibody [N1], N-term

Purification

Purified by antigen-affinity chromatography.

Grade

KO Validated

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Cortactin. The exact sequence is proprietary.

Isotype

IgG
anti-Cortactin (CTTN) (AA 214-513) antibody
CTTN Reactivity: Human WB Host: Rabbit Polyclonal unconjugated

anti-Cortactin (CTTN) (pTyr466) antibody
CTTN Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Cortactin (CTTN) antibody
CTTN Reactivity: Human, Mouse WB, IHC, ELISA Host: Mouse Monoclonal 4C6 unconjugated

anti-Cortactin (CTTN) (AA 341-450) antibody
CTTN Reactivity: Human WB, ELISA, PLA Host: Mouse Monoclonal 2B5 unconjugated

anti-Cortactin (CTTN) (AA 1-509) antibody
CTTN Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Cortactin (CTTN) (AA 1-509) antibody
CTTN Reactivity: Rat WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Cortactin (CTTN) (pTyr421) antibody
CTTN Reactivity: Human, Mouse, Rat WB, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Cortactin (CTTN) (AA 273-302) antibody
CTTN Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB33698 unconjugated

anti-Cortactin (CTTN) (N-Term) antibody
Verified CTTN Reactivity: Human, Mouse, Rat WB, ELISA Host: Goat Polyclonal unconjugated

anti-Cortactin (CTTN) (AA 1-200) antibody
CTTN Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

Application Notes

WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment

Positive Control: NIH-3T3 , JC , PC-12 , mouse brain , rat brain , HeLa
Validation: KO/KD

Restrictions

For Research Use only
Validation #104334 (Multiplex Immunohistochemistry)

by

Akoya Biosciences

No.

#104334

Date

04/20/2021

Antigen

Cortactin

Lot Number

39568

Method validated

Multiplex Immunohistochemistry

Positive Control

Fresh frozen mouse olfactory bulb

Negative Control

Unlabeled control (mouse fresh frozen)

Notes

Passed. The anti-cortactin antibody ABIN2854674 produces high degrees of labeling in granular and external plexiform layers of the mouse olfactory bulb. Additional labeling can be observed in the neuropil of individual glomeruli which is consistent with staining of synapses and neuronal processes.

Validation Images

Murine fresh frozen coronal olfactory bulb section (Thickness = 5 µm) labeled with anti-cortactin antibody ABIN2854674 (green; conjugated to fluorophore ATTO 550). Labeling is present in high degrees of labeling in granular and external plexiform layers with additional staining observed in the glomerular neuropil regions. Grin2b and doublecortin were labeled with ABIN5611338 (red; bound to fluorophore ATTO 550) and a doublecortin antibody (blue; bound to fluorophore AF 488).

FFPE normal human cortex tissue section labeled with anti-cortactin antibody ABIN2854674 (green; bound to fluorophore ATTO 550) after EDTA antigen retrieval. MAP2 and neurofilament were additionally labeled with anti-MAP2 antibody ABIN125739 (red; bound to fluorophore ATTO 550) and anti-neurofilament antibody (blue; bound to fluorophore AF488).
Full Methods
Primary Antibody

ABIN2854674

Secondary Antibody

Full Protocol

Protocol details are described in the Akoya Biosciences CODEX® User Manual (see https://www.akoyabio.com/wp-content/uploads/2021/01/CODEX-User-Manual.pdf).
Tissue preparation as outlined in the Akoya Biosciences CODEX® User Manual fresh-frozen tissue protocol.
Conjugation of the anti-cortactin ABIN2854674 antibody to an oligo barcode used to bind oligo-conjugated fluorophore ATTO 550.

Experimental Notes

No signal was detected in unlabeled specimens.
Specific staining of Cortactin was also observed with human FFPE cortical tissue sections with both citrate antigen retrieval and EDTA antigen retrieval.
Optimal staining and signal to noise ratios were obtained if tissue was pre-treated for autofluorescence removal (see https://www.akoyabio.com/wp-content/uploads/2020/07/Customer-Demonstrated-Protocol-Autofluorescence-Quenching-Mar2020.pdf).
Format

Liquid

Concentration

1 mg/mL

Buffer

1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Target

Cortactin (CTTN)

Alternative Name

cortactin (CTTN Products)

Synonyms

EMS1 antibody, 1110020L01Rik antibody, Ems1 antibody, Cttnb antibody, CG3637 antibody, Dmel\CG3637 antibody, cortactin antibody, cttn antibody, CTTN1 antibody, P85.25 antibody, Cttn antibody, ems1 antibody, CTTN antibody, cortactin antibody, CG3637 gene product from transcript CG3637-RD antibody, cortactin S homeolog antibody, cortactin L homeolog antibody, src substrate cortactin antibody, Src substrate cortactin antibody, CTTN antibody, Cttn antibody, Cortactin antibody, cttn.S antibody, cttn.L antibody, cttn antibody, CpipJ_CPIJ006351 antibody, Bm1_57220 antibody, LOC100533277 antibody

Background

This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene.

Cellular Localization: Cytoplasm , cytoskeleton (By similarity) , Cell projection , lamellipodium (By similarity) , ruffle (By similarity)

Molecular Weight

62 kDa

Gene ID

2017

UniProt

Q14247

Pathways

MAPK Signaling