CD137 antibody (AA 101-200)
(2 references)
(1 validation)-
- Target See all CD137 (TNFRSF9) Antibodies
- CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))
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Binding Specificity
- AA 101-200
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This CD137 antibody is un-conjugated
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Application
- ELISA, Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Cross-Reactivity
- Human, Mouse, Rat
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic peptide derived from human CD137
- Isotype
- IgG
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anti-Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) (AA 19-188) antibody
TNFRSF9 Reactivity: Human ELISA, FACS, WB, IHC, IF, IHC (p), Coat, StM Host: Mouse Monoclonal 4-1BB-3201 unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) (AA 214-255) antibodyTNFRSF9 Reactivity: Human ELISA, FACS, WB Host: Mouse Monoclonal 2A12C2 unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) (AA 24-186) antibodyTNFRSF9 Reactivity: Human ELISA, FACS Host: Rabbit Monoclonal DM66 unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) (AA 40-153) antibodyTNFRSF9 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) (AA 24-186) antibodyTNFRSF9 Reactivity: Human ELISA, FACS, WB, IHC, ICC, Neut Host: Mouse Monoclonal 4G10B9 unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) (AA 150-250) antibodyTNFRSF9 Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) antibodyTNFRSF9 Reactivity: Human, Non-Human Primate ELISA, FACS, IP, Func Host: Mouse Monoclonal 4B4-1 unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) antibodyTNFRSF9 Reactivity: Human, Non-Human Primate ELISA, FACS, IP Host: Mouse Monoclonal 4B4-1 unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) (Internal Region) antibodyTNFRSF9 Reactivity: Human, Rat ELISA, WB, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) antibodyTNFRSF9 Reactivity: Human FACS, WB Host: Rabbit Polyclonal unconjugated
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- Application Notes
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WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400 - Restrictions
- For Research Use only
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- by
- Pharmacology Department, Faculty of Medicine, Granada University
- No.
- #101793
- Date
- 09/13/2017
- Antigen
- CD137
- Lot Number
- AG05152084
- Method validated
- Flow Cytometry
- Positive Control
- rat adipose-derived mesenchymal stem cells
- Negative Control
- cells stained with secondary antibody only
- Notes
Passed. ABIN740445 specifically recognizes Cd137 in rat adipose-derived mesenchymal stem cells.
- Primary Antibody
- ABIN740445
- Secondary Antibody
- donkey anti-rabbit Alexa Fluor 680 conjugated antibody (ThermoFisher Scientific, A10043, lot 1812322)
- Full Protocol
- Grow rat adipose-derived mesenchymal stem cells in DMEM high glucose, GLUTAMAX, pyruvate supplemented (Gibco, 31966021, lot 1774921) supplemented with 10% fetal bovine serum (Gibco, 16000044, lot 1774921) 1% penicillin/streptomycin (Gibco, 15140122, lot 1759446), at 37°C and 5% CO2 in ml on a flask to 95% confluency.
- Wash cells with PBS buffer.
- Detach cells from the flask with trypsin-versene solution (Lonza, H3BE17-161E, lot RB35937) for 3min at 37°C and 5% CO2.
- Centrifuge cells for at 400xg for 5min at RT, aspirate the supernatant.
- Resuspend cells in 1mL of 1% BSA in PBS per 2x106 cells and incubate 20min at RT.
- Incubate cells with primary rabbit anti-CD137 antibody (antibodies-online, ABIN740445, lot AG05152084) diluted 1:35 in 1% BSA in PBS buffer for 20min on ice. Include a negative control without primary antibody.
- Wash cells 1x with PBS buffer and centrifuge them for 400xg for 5min at RT.
- Resuspend cells in 175μl 1% BSA in PBS buffer.
- Incubate cells with secondary donkey anti-rabbit Alexa Fluor 680 conjugated antibody (ThermoFisher Scientific, A10043, lot 1812322) diluted 1:200 in buffer for 15min in ice.
- Wash cells 2xwith PBS buffer and centrifuge them for 400xg for 5min at RT.
- Resuspend the pellet in 500μl PBS buffer.
- Data acquisition in a BD FACSCanto II flow cytometer, equipped with two laser lines of 488nm and 633nm and two scattered light detectors (FSC and SSC). Detect fluorescence in PMTs with filters suitable for the fluorescence of the fluorophore AF680 (735LP and 780/60BP). Threshold is established in FSC. The data is saved in files in FCS format and analyzed offline with the FACSDiva software (BD).
- Experimental Notes
ABIN740445 reveals a specific fluorescence signal in rat adipose-derived mesenchymal stem cells. This is the first time that flow cytometry is performed in this type of cells using this antibody. No signal was registered in the negative control cells.
Validation #101793 (Flow Cytometry)!['Independent Validation' Badge]( "Successfully validated")
!['Independent Validation' Badge]( "Successfully validated")
Validation Images![Flow cytometric analysis of cells stained with ABIN740445 and an AF680-conjugated secondary antibody (A) or the secondary antibody only (B). The top row illustrates the gating for life cells. The second and third row show dots blots and histograms respectively of the positively stained cell populations.]()
Flow cytometric analysis of cells stained with ABIN740445 and an AF680-conjugated secondary antibody (A) or the secondary antibody only (B). The top row illustrates the gating for life cells. The second and third row show dots blots and histograms respectively of the positively stained cell populations.
Full Methods -
- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Expiry Date
- 12 months
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: "Decreased beige adipocyte number and mitochondrial respiration coincide with increased histone methyl transferase (G9a) and reduced FGF21 gene expression in Sprague-Dawley rats fed prenatal low ..." in: The Journal of nutritional biochemistry, Vol. 31, pp. 113-21, (2016) (PubMed).
: "Depletion of white adipocyte progenitors induces beige adipocyte differentiation and suppresses obesity development." in: Cell death and differentiation, (2014) (PubMed).
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: "Decreased beige adipocyte number and mitochondrial respiration coincide with increased histone methyl transferase (G9a) and reduced FGF21 gene expression in Sprague-Dawley rats fed prenatal low ..." in: The Journal of nutritional biochemistry, Vol. 31, pp. 113-21, (2016) (PubMed).
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- Target
- CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))
- Alternative Name
- CD137 (TNFRSF9 Products)
- Synonyms
- TNFRSF9 antibody, zgc:136557 antibody, 4-1BB antibody, CD137 antibody, CDw137 antibody, ILA antibody, A930040I11Rik antibody, AA408498 antibody, AI325004 antibody, Cd137 antibody, Ly63 antibody, tumor necrosis factor receptor superfamily member 9 antibody, TNF receptor superfamily member 9 antibody, tumor necrosis factor receptor superfamily, member 9a antibody, tumor necrosis factor receptor superfamily, member 9 antibody, TNFRSF9 antibody, tnfrsf9a antibody, Tnfrsf9 antibody
- Background
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Synonyms: ILA, 4-1BB, CD137, CDw137, Tumor necrosis factor receptor superfamily member 9, 4-1BB ligand receptor, T-cell antigen 4-1BB homolog, T-cell antigen ILA, TNFRSF9
Background: Receptor for TNFSF9/4-1BBL. Possibly active during T cell activation.
- Gene ID
- 3604
- UniProt
- Q07011
- Pathways
- Cancer Immune Checkpoints
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