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NR2C1 antibody (N-Term)

NR2C1 Reactivity: Human, Mouse, Rat, Dog, Horse, Rabbit, Pig, Monkey WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6741570
  • Target See all NR2C1 Antibodies
    NR2C1 (Nuclear Receptor Subfamily 2, Group C, Member 1 (NR2C1))
    Binding Specificity
    • 13
    • 12
    • 9
    • 9
    • 7
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    • 65
    • 16
    • 11
    • 9
    • 8
    • 8
    • 8
    • 7
    • 6
    • 3
    • 3
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Dog, Horse, Rabbit, Pig, Monkey
    Host
    • 58
    • 8
    Rabbit
    Clonality
    • 62
    • 4
    Polyclonal
    Conjugate
    • 39
    • 5
    • 4
    • 4
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This NR2C1 antibody is un-conjugated
    Application
    • 57
    • 30
    • 8
    • 4
    • 4
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Brand
    IHC-plus™
    Specificity
    Human NR2C1 / TR2-11
    Predicted Reactivity
    Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit, Horse, Pig (100%) Bovine, Guinea pig (92%) Chicken (84%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic peptide from N-Terminus of human NR2C1 (Q15626, NP_001027458). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Rabbit, Horse, Pig (100%), Hamster, Bovine, Opossum, Guinea pig, Platypus, Lizard, Zebrafish (92%), Bat, Turkey, Zebra finch, Chicken, Xenopus (84%).

    Type of Immunogen: Synthetic peptide
    Isotype
    IgG
  • Application Notes
    Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.
    Comment

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Distilled water
    Concentration
    Lot specific
    Buffer
    Lyophilized from PBS with 2 % sucrose
    Handling Advice
    Avoid repeat freeze-thaw cycles.
    Storage
    4 °C,-20 °C
    Storage Comment
    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target
    NR2C1 (Nuclear Receptor Subfamily 2, Group C, Member 1 (NR2C1))
    Alternative Name
    NR2C1 (NR2C1 Products)
    Synonyms
    TR2 antibody, NR2C1 antibody, dor2 antibody, tr2-11 antibody, xDOR2 antibody, DKFZp468M0512 antibody, si:dkey-183c16.2 antibody, zgc:110672 antibody, 4831444H07Rik antibody, 80.3 antibody, Eenr antibody, Tr2-11 antibody, Tr2 antibody, Psg4 antibody, TR2-11 antibody, PSG9 antibody, nr2c1 antibody, nuclear receptor subfamily 2 group C member 1 antibody, nuclear receptor subfamily 2 group C member 1 S homeolog antibody, nuclear receptor subfamily 2, group C, member 1 antibody, pregnancy specific beta-1-glycoprotein 4 antibody, nuclear receptor subfamily 2 group C member 1 L homeolog antibody, NR2C1 antibody, nr2c1.S antibody, nr2c1 antibody, Nr2c1 antibody, PSG4 antibody, nr2c1.L antibody
    Background
    Name/Gene ID: NR2C1
    Subfamily: NR2 Hepatocyte NF4-like
    Family: NHR

    Synonyms: NR2C1, Orphan nuclear receptor TR2, Testicular orphan receptor-2, TR2, TR2 nuclear hormone receptor, TR2-11, Testicular receptor 2
    Gene ID
    7181
    NCBI Accession
    NP_001027458
    UniProt
    P13056
    Pathways
    Nuclear Receptor Transcription Pathway, Retinoic Acid Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway
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