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CCT5 antibody

Name: CCT5 antibody
Brand: antibodies-online
SKU: ABIN2856739
Price: 609.18 USD
Availability: InStock

KO Validated CCT5 Reactivity: Human WB, IP, IF, IHC (p), ICC, IHC (fro) Host: Rabbit Polyclonal unconjugated

WB Image Sample (50 ug of whole cell lysate) A: mouse brain 7.

IHC-P Image Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using TCP-1 epsilon, antibody at 1:500 dilution.

IHC

WB Image TCP1 epsilon antibody detects TCP1 protein by western blot analysis.

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TCP-1 epsilon, antibody at 1:200 dilution.

IP Image Immunoprecipitation of TCP1 epsilon protein from 293T whole cell extracts using 5 μg of TCP1 epsilon antibody [N1C1], Western blot analysis was performed using TCP1 epsilon antibody [N1C1], EasyBlot anti-Rabbit IgG was used as a secondary reagent.

WB Image Sample (30 ug of whole cell lysate) A: Molt-4 , 7.

6 images

Catalog No. ABIN2856739

Datasheet as PDF

Target See all CCT5 Antibodies

CCT5 (Chaperonin Containing TCP1, Subunit 5 (Epsilon) (CCT5))
Reactivity
50

35

24

7

6

6

5

4

4

3

3

3

2

2

2

2
Human
Host
45

3

3
Rabbit
Compatible Secondaries
Clonality
45

5
Polyclonal
Conjugate
34

3

2

2

2

2

2

2

1

1
This CCT5 antibody is un-conjugated
Application
33

14

12

10

10

8

6

3

1

1

1

1
Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity

Human, Mouse, Zebrafish (Danio rerio)

Characteristics

Rabbit Polyclonal antibody to TCP1 epsilon (chaperonin containing TCP1, subunit 5 (epsilon))
TCP1 epsilon antibody [N1C1]

Purification

Purified by antigen-affinity chromatography.

Grade

KO Validated

Immunogen

Recombinant protein encompassing a sequence within the center region of human TCP1 epsilon. The exact sequence is proprietary.

Isotype

IgG
anti-Chaperonin Containing TCP1, Subunit 5 (Epsilon) (CCT5) (AA 1-541) antibody
CCT5 Reactivity: Human WB, ELISA, IF, IHC (p) Host: Mouse Monoclonal 4E5-4B1 unconjugated

anti-Chaperonin Containing TCP1, Subunit 5 (Epsilon) (CCT5) (AA 1-270) antibody
CCT5 Reactivity: Human WB, ELISA, IHC, IP Host: Rabbit Polyclonal unconjugated

anti-Chaperonin Containing TCP1, Subunit 5 (Epsilon) (CCT5) antibody
KO Validated CCT5 Reactivity: Human WB, IF, IHC (p), ICC, IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Chaperonin Containing TCP1, Subunit 5 (Epsilon) (CCT5) (AA 443-535) antibody
CCT5 Reactivity: Human, Mouse, Rat WB, IHC (p), ICC Host: Rabbit Polyclonal unconjugated

anti-Chaperonin Containing TCP1, Subunit 5 (Epsilon) (CCT5) (AA 1-542) antibody
CCT5 Reactivity: Human WB, ELISA, IHC, IF, IHC (p) Host: Mouse Monoclonal 4E5-4B1 unconjugated

anti-Chaperonin Containing TCP1, Subunit 5 (Epsilon) (CCT5) (AA 1-224) antibody
CCT5 Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Xenopus laevis, Chicken WB, IF, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Chaperonin Containing TCP1, Subunit 5 (Epsilon) (CCT5) (AA 1-270) antibody
CCT5 Reactivity: Human WB, ELISA, IF Host: Rabbit Polyclonal unconjugated

anti-Chaperonin Containing TCP1, Subunit 5 (Epsilon) (CCT5) (Internal Region) antibody
CCT5 Reactivity: Human, Mouse, Rat WB, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Chaperonin Containing TCP1, Subunit 5 (Epsilon) (CCT5) (Internal Region) antibody
CCT5 Reactivity: Human, Mouse, Rat WB, ELISA Host: Rabbit Polyclonal unconjugated

anti-Chaperonin Containing TCP1, Subunit 5 (Epsilon) (CCT5) antibody
CCT5 Reactivity: Human, Mouse, Rat ELISA, IHC Host: Rabbit Polyclonal unconjugated

Application Notes

WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment

Positive Control: Mouse brain , Molt-4 , 293T
Validation: KO/KD

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative

Thimerosal (Merthiolate)

Precaution of Use

This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Target

CCT5 (Chaperonin Containing TCP1, Subunit 5 (Epsilon) (CCT5))

Alternative Name

chaperonin containing TCP1 subunit 5 (CCT5 Products)

Synonyms

CCT-epsilon antibody, CCTE antibody, TCP-1-epsilon antibody, wu:fb54h08 antibody, CCT5 antibody, Cct5 antibody, DKFZp469K0732 antibody, cct5a antibody, Ccte antibody, TCPE antibody, mKIAA0098 antibody, chaperonin containing TCP1 subunit 5 antibody, chaperonin containing TCP1, subunit 5 (epsilon) antibody, chaperonin containing TCP1 subunit 5 S homeolog antibody, Chaperonin Containing TCP-1 antibody, T-complex protein 1 subunit epsilon antibody, chaperonin containing Tcp1, subunit 5 (epsilon) antibody, chaperonin containing TCP1, subunit 5 (epsilon) b antibody, CCT5 antibody, Cct5 antibody, cct5 antibody, cct5.S antibody, cct-5 antibody, LOC100632800 antibody, cct5b antibody

Background

This gene encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

Cellular Localization: Cytoplasm

Molecular Weight

60 kDa

Gene ID

22948

UniProt

P48643