CHFR antibody
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- Target See all CHFR Antibodies
- CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This CHFR antibody is un-conjugated
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Application
- ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
- Specificity
- CHFR Antibody detects endogenous levels of total CHFR
- Cross-Reactivity
- Human, Mouse (Murine), Rat (Rattus)
- Purification
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- Immunogen
- A synthesized peptide
- Isotype
- IgG
- Top Product
- Discover our top product CHFR Primary Antibody
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anti-Checkpoint with Forkhead and Ring Finger Domains (CHFR) (AA 1-652) antibody
CHFR Reactivity: Human ELISA, WB, IHC (p), RNAi Host: Mouse Monoclonal 1H3-A12 unconjugated
anti-Checkpoint with Forkhead and Ring Finger Domains (CHFR) (AA 141-422) antibodyCHFR Reactivity: Human ELISA, WB, IHC Host: Rabbit Polyclonal unconjugated
anti-Checkpoint with Forkhead and Ring Finger Domains (CHFR) (AA 136-194) antibodyCHFR Reactivity: Human ELISA, WB Host: Mouse Monoclonal 2B11 unconjugated
anti-Checkpoint with Forkhead and Ring Finger Domains (CHFR) (C-Term) antibodyCHFR Reactivity: Human WB Host: Rabbit Polyclonal unconjugated
anti-Checkpoint with Forkhead and Ring Finger Domains (CHFR) (AA 36-85) antibodyCHFR Reactivity: Human, Mouse, Rat, Rabbit, Zebrafish (Danio rerio), Guinea Pig, Monkey WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Checkpoint with Forkhead and Ring Finger Domains (CHFR) antibodyCHFR Reactivity: Human ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-Checkpoint with Forkhead and Ring Finger Domains (CHFR) (C-Term) antibodyVerified CHFR Reactivity: Human ELISA, IHC Host: Goat Polyclonal unconjugated
anti-Checkpoint with Forkhead and Ring Finger Domains (CHFR) (AA 1-290) antibodyCHFR Reactivity: Human WB Host: Rabbit Polyclonal unconjugated
anti-Checkpoint with Forkhead and Ring Finger Domains (CHFR) (N-Term) antibodyCHFR Reactivity: Human, Mouse, Rat, Rabbit, Cow, Zebrafish (Danio rerio), Horse, Dog, Guinea Pig WB, IHC Host: Rabbit Polyclonal unconjugated
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- Application Notes
- WB 1:500~1:1000, IF/ICC 1:100-1:500
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20 °C.Stable for 12 months from date of receipt
- Expiry Date
- 12 months
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- Target
- CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))
- Alternative Name
- CHFR (CHFR Products)
- Synonyms
- RNF116 antibody, RNF196 antibody, CHFR antibody, fc43f10 antibody, si:dkey-69h6.7 antibody, wu:fc43f10 antibody, rnf116 antibody, rnf196 antibody, 5730484M20Rik antibody, C230082M18 antibody, checkpoint with forkhead and ring finger domains antibody, checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase antibody, checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase L homeolog antibody, CHFR antibody, chfr antibody, Chfr antibody, chfr.L antibody
- Background
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Description: E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress.
Gene: CHFR
- Molecular Weight
- 73 kDa
- Gene ID
- 55743
- UniProt
- Q96EP1
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