FLT3LG Protein (AA 27-185) (Fc Tag)
-
- Target See all FLT3LG Proteins
- FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 27-185
-
Origin
- Human
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This FLT3LG protein is labelled with Fc Tag.
- Purpose
- Human FLT3 Ligand Protein
- Sequence
- Thr27-Pro185
- Characteristics
- Recombinant Human FLT3 Ligand Protein is expressed from HEK293 with hFc at the C-Terminus.It contains Thr27-Pro185.
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human FLT3, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human FLT3 Ligand, hFc Tag with the EC50 of 18.7ng/ml determined by ELISA. The affinity constant of 35.07 nM as determined in SPR assay (Biacore T200). See testing image for detail.
-
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) (AA 27-185) protein (His tag)
FLT3LG Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) (AA 27-189) protein (Fc Tag)FLT3LG Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) protein (mFc-His Tag)FLT3LG Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) (AA 27-185) protein (His tag)FLT3LG Origin: Human Host: Hi-5 Cells Recombinant >90 % as determined by SDS-PAGE.
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) (AA 28-189) protein (His tag)FLT3LG Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) (AA 27-181) protein (His tag)FLT3LG Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) (AA 1-235) protein (GST tag)FLT3LG Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) (AA 27-136) protein (GST tag)FLT3LG Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) (AA 27-184) protein (His tag)Crystallography grade FLT3LG Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) (AA 27-189) protein (His tag)Crystallography grade FLT3LG Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose and 0.01 % tween is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
-
- Target
- FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))
- Alternative Name
- FLT3 Ligand (FLT3LG Products)
- Synonyms
- Flt3lg Protein, Ly72L Protein, FL Protein, FLT3L Protein, Vegfr3 Protein, fms related tyrosine kinase 3 ligand Protein, FMS-like tyrosine kinase 3 ligand Protein, fms-related tyrosine kinase 4 Protein, fms-related tyrosine kinase 3 ligand Protein, FLT3LG Protein, Flt3l Protein, Flt4 Protein, Flt3lg Protein
- Background
- Flt-3 Ligand, also known as FL, is an alpha -helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages.Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.
- Molecular Weight
- 44.8 kDa. Due to glycosylation, the protein migrates to 90-118 kDa based on Tris-Bis PAGE result.
- Pathways
- RTK Signaling
-
