PSMA antibody (AA 44-750)
(9 references)-
- Target See all PSMA (FOLH1) Antibodies
- PSMA (FOLH1) (Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1))
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Binding Specificity
- AA 44-750
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Reactivity
- Human, Mouse, Rat, Pig
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This PSMA antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
- Specificity
- The mouse monoclonal antibody GCP-04 recognizes amino acids 100-104 of extracellular domain of denaturated glutamate carboxypeptidase II (PSMA, NAALADase, FOLH1), an approximately 95-110 kDa transmembrane glycoprotein.
- Cross-Reactivity (Details)
- Human, Porcine, Mouse, Rat, Other not determined
- Purification
- Purified by protein-A affinity chromatography.
- Purity
- > 95 % (by SDS-PAGE)
- Immunogen
- Recombinant fragment of human GCPII (amino acids 44-750) produced in S2 cells
- Clone
- GCP-04
- Isotype
- IgG1
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anti-Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1) antibody
FOLH1 Reactivity: Human, Mouse, Rat IHC Host: Rabbit Polyclonal unconjugated
anti-Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1) (AA 274-587) antibodyFOLH1 Reactivity: Human WB, IHC, ICC, IP Host: Rabbit Polyclonal unconjugated
anti-Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1) (AA 44-177) antibodyFOLH1 Reactivity: Human WB, IHC, ELISA, FACS, ICC, Neut Host: Mouse Monoclonal 2D10E10 unconjugated
anti-Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1) (AA 275-588) antibodyFOLH1 Reactivity: Pig WB, IHC, ICC, IP Host: Rabbit Polyclonal unconjugated
anti-Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1) (AA 44-750) antibodyFOLH1 Reactivity: Human FACS, ICC, IP, CyTOF Host: Mouse Monoclonal GCP-05 unconjugated
anti-Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1) (AA 232-433) antibodyFOLH1 Reactivity: Human WB, IHC, StM Host: Mouse Monoclonal FOLH1-2354 unconjugated
anti-Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1) (AA 161-190) antibodyFOLH1 Reactivity: Human WB, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated
anti-Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1) (AA 161-190), (N-Term) antibodyFOLH1 Reactivity: Human WB, IHC (p), IF Host: Rabbit Polyclonal RB33647 unconjugated
anti-Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1) (AA 1-719) antibodyFOLH1 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 3B5 unconjugated
anti-Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1) antibodyFOLH1 Reactivity: Human, Mouse, Rat, Dog, Monkey WB, IF Host: Mouse Monoclonal 2E1 unconjugated
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- Application Notes
- Western blotting: Recommended dilution: 1 μg/mL, positive control: LNCaP cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 mL cold lysis buffer (1 % NP-40). Incubate 30 min on ice. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer. Both reducing and non-reducing conditions.
- Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Do not freeze.
- Storage
- 4 °C
- Storage Comment
- Store at 2-8°C. Do not freeze.
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: "Prostate-Specific Membrane Antigen in Anaplastic and Poorly Differentiated Thyroid Cancer-A New Diagnostic and Therapeutic Target?" in: Cancers, Vol. 13, Issue 22, (2021) (PubMed).
: "Prostate specific membrane antigen (PSMA) in urothelial cell carcinoma (UCC) is associated with tumor grading and staging." in: Journal of cancer research and clinical oncology, (2020) (PubMed).
: "Mice lacking glutamate carboxypeptidase II develop normally, but are less susceptible to traumatic brain injury." in: Journal of neurochemistry, Vol. 134, Issue 2, pp. 340-53, (2015) (PubMed).
: "Comparative analysis of monoclonal antibodies against prostate-specific membrane antigen (PSMA)." in: The Prostate, Vol. 74, Issue 16, pp. 1674-90, (2014) (PubMed).
: "Tissue expression and enzymologic characterization of human prostate specific membrane antigen and its rat and pig orthologs." in: The Prostate, Vol. 68, Issue 2, pp. 171-82, (2007) (PubMed).
: "Expression of glutamate carboxypeptidase II in human brain." in: Neuroscience, Vol. 144, Issue 4, pp. 1361-72, (2007) (PubMed).
: "Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding." in: European journal of biochemistry / FEBS, Vol. 271, Issue 13, pp. 2782-90, (2004) (PubMed).
: "Identification of the N-glycosylation sites on glutamate carboxypeptidase II necessary for proteolytic activity." in: Protein science : a publication of the Protein Society, Vol. 13, Issue 6, pp. 1627-35, (2004) (PubMed).
: "Substrate specificity, inhibition and enzymological analysis of recombinant human glutamate carboxypeptidase II." in: Journal of neurochemistry, Vol. 80, Issue 3, pp. 477-87, (2002) (PubMed).
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: "Prostate-Specific Membrane Antigen in Anaplastic and Poorly Differentiated Thyroid Cancer-A New Diagnostic and Therapeutic Target?" in: Cancers, Vol. 13, Issue 22, (2021) (PubMed).
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- Target
- PSMA (FOLH1) (Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1))
- Alternative Name
- GCPII / PSMA (FOLH1 Products)
- Synonyms
- FGCP antibody, FOLH antibody, GCP2 antibody, GCPII antibody, NAALAD1 antibody, NAALAdase antibody, PSM antibody, PSMA antibody, mGCP antibody, mopsm antibody, Naalad antibody, putative N-acetylated-alpha-linked acidic dipeptidase antibody, folate hydrolase 1B antibody, folate hydrolase 1 antibody, folate hydrolase (prostate-specific membrane antigen) 1 antibody, LOC451185 antibody, FOLH1B antibody, FOLH1 antibody, Folh1 antibody
- Background
- Folate hydrolase 1,Glutamate carboxypeptidase II (GCPII), also known as N-acetyl-alpha-linked acidic dipeptidase I (NAALADase I), folate hydrolase (FOLH1), and prostate-specific membrane antigen (PSMA), is an approximately 95-110 kDa type II transmembrane glycoprotein expressed in various tissues. In nervous system GCPII cleaves abundant N-acetylaspartylglutamate, which is released from neurons in a calcium-dependent manner, to N-acetylaspartate and glutamate. As immoderate glutamate concentration is neurotoxic, GCPII contributes to pathological conditions regarding e.g. Alzheimer´s disease, Huntington´s disease, epilepsy, schizophrenia, stroke or neuropathic pain and appears to be an interesting therapeutic target. In jejunum GCPII hydrolyzes pteroylpoly-gamma-glutamate to folate and glutamate, enabling folate to be absorbed by gastrointestinal tract. GCPII, which is present in a number of tissues at low levels, is overexpressed in neovasculature of most solid tumours and is a target enzyme for diagnosis and treatment of prostate cancer. Normal human prostate express more mRNA coding for a cytosolic GCPII form truncated at the N-terminus (PSM´) than mRNA for membrane-bound GCPII, and this ratio is reversed upon malignant transformation.,GCP2, FOLH1, NAALADase I, PGGCP, FGGCP, FGCP,
- Gene ID
- 2346
- UniProt
- Q04609
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