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CD73 antibody (C-Term)

Name: CD73 antibody (C-Term)
SKU: ABIN388750
Price: 429 USD
Availability: InStock

NT5E Reactivity: Human, Mouse WB, IHC (p) Host: Rabbit Polyclonal RB02846 unconjugated

Western blot analysis of NT5E (arrow) using rabbit polyclonal NT5E Antibody (ABIN388750 and ABIN2839011).

Formalin-fixed and paraffin-embedded human Placenta tissue reacted with CD73 (NT5E) Antibody (C-term) (ABIN388750 and ABIN2839011) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

IHC (p)

Western blot analysis of CD73 Pab (ABIN388750 and ABIN2839011) in mouse brain tissue lysate.

Western blot analysis of lysates from U266B1,HepG2 cell line,mouse brain tissue lysate(from left to right), using NT5E- Antibody (ABIN388750 and ABIN2839011).

All lanes : Anti-NT5E Antibody at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: U266B1 whole cell lysate Lysates/proteins at 20 μg per lane.

5 images

(10 references)

Catalog No. ABIN388750

Datasheet as PDF

Target See all CD73 (NT5E) Antibodies

CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
Binding Specificity
16

9

8

6

5

5

5

4

4

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 520-550, C-Term
Reactivity
101

50

26

18

18

16

4

2

1

1

1

1

1
Human, Mouse
Host
81

31

9

2
Rabbit
Compatible Secondaries
Clonality
72

51
Polyclonal
Conjugate
58

14

11

7

7

4

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1
This CD73 antibody is un-conjugated
Application
83

52

48

40

17

14

14

14

12

7

5

3

3

2

2

1

1

1

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen

This CD73 (NT5E) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 520-550 amino acids from the C-terminal region of human CD73 (NT5E).

Clone

RB02846

Isotype

Ig Fraction
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 30-250) antibody
NT5E Reactivity: Human WB, IHC, ELISA Host: Mouse Monoclonal 4G6B10 unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 29-500) antibody
NT5E Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 30-250) antibody
NT5E Reactivity: Human WB, FACS, IHC, ELISA Host: Mouse Monoclonal 4G6E3 unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) antibody
NT5E Reactivity: Human WB, FACS, IHC, ELISA, IF, StM, Coat Host: Mouse Monoclonal NT5E-2545 unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) antibody
NT5E Reactivity: Human WB, FACS, IHC, IF, StM Host: Mouse Monoclonal NT5E-2503 unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 290-550) antibody
NT5E Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) antibody (PE)
NT5E Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal AD2 PE

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 48-527) antibody
NT5E Reactivity: Cow WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) antibody
NT5E Reactivity: Human, Mouse, Rat IHC Host: Rabbit Polyclonal unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 29-500) antibody
NT5E Reactivity: Human WB, IHC, IP, ICC Host: Mouse Monoclonal D1 unconjugated

Application Notes

WB: 1:1000. WB: 1:1000. WB: 1:500. WB: 1:1000. IHC-P: 1:10~50

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid freeze-thaw cycles.

Storage

4 °C,-20 °C

Storage Comment

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Expiry Date

6 months
Soleimanifar, Mortazavi, Nadri, Soleimani: "Conjunctiva derived mesenchymal stem cell (CJMSCs) as a potential platform for differentiation into corneal epithelial cells on bioengineered electrospun scaffolds." in: Journal of biomedical materials research. Part A, Vol. 105, Issue 10, pp. 2703-2711, (2018) (PubMed).

Yu, Wang, Lu, Wang, Li, Liao, Zhu, Lv, Zhi, Yu, Jin, Zou, Ou, Liu, Zhou: "Extracellular 5'-nucleotidase (CD73) promotes human breast cancer cells growth through AKT/GSK-3β/β-catenin/cyclinD1 signaling pathway." in: International journal of cancer, Vol. 142, Issue 5, pp. 959-967, (2018) (PubMed).

Tak, Jung, Kim, Park, Jun, Lee, Jung, Kirchner, Hwang, Song, Lee: "Protective role of hypoxia-inducible factor-1α-dependent CD39 and CD73 in fulminant acute liver failure." in: Toxicology and applied pharmacology, Vol. 314, pp. 72-81, (2017) (PubMed).

Cartón-García, Overeem, Nieto, Bazzocco, Dopeso, Macaya, Bilic, Landolfi, Hernandez-Losa, Schwartz, Ramon Y Cajal, van Ijzendoorn, Arango: "Myo5b knockout mice as a model of microvillus inclusion disease." in: Scientific reports, Vol. 5, pp. 12312, (2015) (PubMed).

Zhi, Wang, Yu, Yu, Zhang, Yin, Zhou: "Potential prognostic biomarker CD73 regulates epidermal growth factor receptor expression in human breast cancer." in: IUBMB life, Vol. 64, Issue 11, pp. 911-20, (2012) (PubMed).

Hart, Grenz, Gorzolla, Schittenhelm, Dalton, Eltzschig: "Hypoxia-inducible factor-1?-dependent protection from intestinal ischemia/reperfusion injury involves ecto-5'-nucleotidase (CD73) and the A2B adenosine receptor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 7, pp. 4367-74, (2011) (PubMed).

Zhi, Wang, Zhou, Yu, Jian, Tang, Yin, Zhou: "RNAi-mediated CD73 suppression induces apoptosis and cell-cycle arrest in human breast cancer cells." in: Cancer science, Vol. 101, Issue 12, pp. 2561-9, (2010) (PubMed).

Leth-Larsen, Lund, Hansen, Laenkholm, Tarin, Jensen, Ditzel: "Metastasis-related plasma membrane proteins of human breast cancer cells identified by comparative quantitative mass spectrometry." in: Molecular & cellular proteomics : MCP, Vol. 8, Issue 6, pp. 1436-49, (2009) (PubMed).

Hart, Henn, Köhler, Kloor, Mittelbronn, Gorzolla, Stahl, Eltzschig: "Role of extracellular nucleotide phosphohydrolysis in intestinal ischemia-reperfusion injury." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 22, Issue 8, pp. 2784-97, (2008) (PubMed).

Macdonald, Pike: "A simplified method for the preparation of detergent-free lipid rafts." in: Journal of lipid research, Vol. 46, Issue 5, pp. 1061-7, (2005) (PubMed).
Target

CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Alternative Name

CD73 (NT5E) (NT5E Products)

Synonyms

CALJA antibody, CD73 antibody, E5NT antibody, NT antibody, NT5 antibody, NTE antibody, eN antibody, eNT antibody, zgc:63784 antibody, NT5E antibody, MGC114869 antibody, PSPTO2277 antibody, 2210401F01Rik antibody, AI447961 antibody, Nt5 antibody, 5'-nucleotidase ecto antibody, 5'-nucleotidase, ecto (CD73) antibody, 5'-nucleotidase antibody, 5' nucleotidase, ecto antibody, NT5E antibody, nt5e antibody, nt5e.S antibody, PSPTO_2277 antibody, CBU_1233 antibody, MCA1322 antibody, Nt5e antibody

Background

Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70 kD subunits bound externally to the plasma membrane by a glycosyl phosphatidyl inositol linkage. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5E occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. It is not known whether the different enzymes are coded by different genes or result from different posttranslational modifications of a single coding sequence.

Molecular Weight

63368

Gene ID

4907

NCBI Accession

NP_001191742, NP_002517

UniProt

P21589

Pathways

Synaptic Membrane, Ribonucleoside Biosynthetic Process