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NQO1 antibody (C-Term)

Verified NQO1 Reactivity: Human, Rat WB, ELISA, IHC, IF Host: Goat Polyclonal unconjugated
Catalog No. ABIN184714
  • Target See all NQO1 Antibodies
    NQO1 (NAD(P)H Dehydrogenase, Quinone 1 (NQO1))
    Binding Specificity
    • 16
    • 6
    • 5
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 63
    • 32
    • 32
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    Human, Rat
    Host
    • 55
    • 7
    • 2
    • 1
    Goat
    Clonality
    • 48
    • 17
    Polyclonal
    Conjugate
    • 39
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This NQO1 antibody is un-conjugated
    Application
    • 52
    • 23
    • 14
    • 14
    • 11
    • 11
    • 10
    • 9
    • 8
    • 7
    • 4
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)
    Purpose
    NQO1
    Sequence
    SIPTDNQIKA RK
    Specificity
    This products is expected to recognize all three reported isoforms (NP_000894.1, NP_001020604.1 and NP_001020605.1).
    Cross-Reactivity
    Dog, Human, Pig, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-SIPTDNQIKARK, from the C Terminus of the protein sequence according to NP_000894.1, NP_001020604.1, NP_001020605.1.
    Isotype
    IgG
    Top Product
    Discover our top product NQO1 Primary Antibody
  • Application Notes

    Immunohistochemistry: In paraffin embedded Human Kidney shows specific staining of the glomeruli. Recommended concentration: 2-4 μg/mL.

    Western Blot: Approx. 30 kDa band observed in lysates of cell lines U251 and HepG2 and in Human Kidney, Lung and Duodenum lysates, and approx. 30-32 kDa band in Rat Kidney, Lung and Duodenum lysates (calculated MW of 30.9 kDa according to Human NP_000894.1

    Peptide ELISA: antibody detection limit dilution 1:64000.

    Comment

    Immunofluorescence: Expression of the protein seen in the cytoplasm and nucleus of HepG2 cells. Recommended concentration: 10μg/ml.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    NQO1 (NAD(P)H Dehydrogenase, Quinone 1 (NQO1))
    Alternative Name
    NQO1 (NQO1 Products)
    Synonyms
    zgc:77191 antibody, wu:fb63c10 antibody, nqo1 antibody, DHQU antibody, DIA4 antibody, DTD antibody, NMOR1 antibody, NMORI antibody, QR1 antibody, Dia4 antibody, AV001255 antibody, Dtd antibody, Nmo-1 antibody, Nmo1 antibody, Nmor1 antibody, Ox-1 antibody, Ox1 antibody, Qr1 antibody, NADPH-d antibody, NAD(P)H dehydrogenase, quinone 1 antibody, NAD(P)H quinone dehydrogenase 1 antibody, NAD(P)H dehydrogenase, quinone 1 L homeolog antibody, nqo1 antibody, NQO1 antibody, Bpet2092 antibody, nqo1.L antibody, Nqo1 antibody
    Background
    NQO1, NAD(P)H dehydrogenase, quinone 1, DTD, QR1, DHQU, DIA4, NMOR1, NMORI, diaphorase-4, diaphorase (NADH/NADPH), diaphorase (NADH/NADPH) (cytochrome b-5 reductase), NAD(P)H:menadione oxidoreductase 1, dioxin-inducible 1, DT-diaphorase, NAD(P)H menadione
    Gene ID
    1728, 18104
    NCBI Accession
    NP_000894, NP_001020604, NP_001020605
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