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Hsc70 antibody (C-Term)

Name: Hsc70 antibody (C-Term)
Brand: antibodies-online
SKU: ABIN2854950
Price: 609.18 USD
Availability: InStock

HSPA8 Reactivity: Human, Rat, Mouse WB, ICC, IF, IHC (p) Host: Rabbit Polyclonal unconjugated

WB Image Sample (50 ug of whole cell lysate) A: Mouse brain 7.

ICC/IF Image Immunofluorescence analysis of methanol-fixed HeLa, using HSC70, antibody at 1:200 dilution.

IHC-P Image HSC70 antibody detects HSC70 protein at nucleolus on rat hind brain by immunohistochemical analysis.

IHC

IHC-P Image HSC70 antibody detects HSC70 protein at nucleolus on mouse prostate by immunohistochemical analysis.

IHC

IHC-P Image Immunohistochemical analysis of paraffin-embedded U87 xenograft, using HSC70, antibody at 1:100 dilution.

IHC

WB Image Sample (30 ug of whole cell lysate) A: H1299 7.

6 images

Catalog No. ABIN2854950

Datasheet as PDF

Target See all Hsc70 (HSPA8) Antibodies

Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))
Binding Specificity
14

6

6

4

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
C-Term
Reactivity
82

45

41

21

17

14

13

11

11

10

10

10

5

5

4

4

4

3

3

3

2

1

1

1

1

1
Human, Rat, Mouse
Host
71

27

7

2
Rabbit
Compatible Secondaries
Clonality
69

37
Polyclonal
Conjugate
59

19

11

3

3

1

1

1

1

1

1

1

1

1

1

1

1
This Hsc70 antibody is un-conjugated
Application
93

53

40

32

29

29

16

15

11

11

11

5

4

2

2

2

2

1

1
Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity

Pig (Porcine), Rhesus Monkey

Cross-Reactivity (Details)

Pig (100 %), Rhesus Monkey (100 %)

Characteristics

Rabbit Polyclonal antibody to Hsc70 (heat shock 70 kDa protein 8)
HSC70 antibody

Purification

Affinity purified by Protein A.

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human HSC70. The exact sequence is proprietary.

Isotype

IgG

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anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 1-300) antibody
HSPA8 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) antibody
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anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 539-569), (C-Term) antibody
HSPA8 Reactivity: Human WB, IF, IHC (p), FACS Host: Rabbit Polyclonal RB16528 unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 82-110), (N-Term) antibody
HSPA8 Reactivity: Human WB, IF, IHC (p), FACS Host: Rabbit Polyclonal RB17655 unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 13-31), (N-Term) antibody
HSPA8 Reactivity: Human, Rat, Mouse WB, ICC, IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (N-Term) antibody
HSPA8 Reactivity: Human, Rat, Mouse, Cow, Sheep, Pig, Zebrafish (Danio rerio), Goat WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 534-615) antibody
HSPA8 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 418-597) antibody
HSPA8 Reactivity: Human WB, IHC, ELISA Host: Rabbit Polyclonal unconjugated

Application Notes

Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:1000-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:1000-1:10000*

Comment

Positive Control: 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain

Restrictions

For Research Use only
Format

Liquid

Concentration

10 mg/mL

Buffer

1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative

Thimerosal (Merthiolate)

Precaution of Use

This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Target

Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Alternative Name

HSC70 (HSPA8 Products)

Synonyms

hsc54 antibody, hsc70 antibody, hsc71 antibody, hsp71 antibody, hsp73 antibody, hspa10 antibody, lap1 antibody, nip71 antibody, HSC54 antibody, HSC70 antibody, HSC71 antibody, HSP71 antibody, HSP73 antibody, HSPA10 antibody, LAP1 antibody, NIP71 antibody, Hsc70 antibody, 2410008N15Rik antibody, Hsc71 antibody, Hsc73 antibody, Hsp73 antibody, Hspa10 antibody, wu:fb01g06 antibody, wu:fi48b06 antibody, heat shock protein family A (Hsp70) member 8 L homeolog antibody, heat shock protein family A (Hsp70) member 8 antibody, heat shock 70kDa protein 8 antibody, heat shock protein 8 antibody, hspa8.L antibody, HSPA8 antibody, Hspa8 antibody, hspa8 antibody

Background

The product encoded by this gene belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. This gene encodes a heat-shock cognate protein. This protein binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Two alternatively spliced variants have been characterized to date.

Cellular Localization: Cytoplasm , Melanosome

Molecular Weight

71 kDa

Gene ID

3312