TUBB3 antibody (AA 443-450)
(1 reference)
(1 validation)-
- Target See all TUBB3 Antibodies
- TUBB3 (Tubulin, beta 3 (TUBB3))
-
Binding Specificity
- AA 443-450
-
Reactivity
- Human, Mouse, Rat
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This TUBB3 antibody is un-conjugated
-
Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
- Specificity
- Specific for beta3-tubulin.
- Purification
- antiserum
- Immunogen
- Synthetic peptide (aa 443-450 of mouse beta3-tubulin) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine.
- Top Product
- Discover our top product TUBB3 Primary Antibody
-
anti-Tubulin, beta 3 (TUBB3) (N-Term) antibody
TUBB3 Reactivity: Human, Mouse, Rat, Dog, Pig WB, FACS, IHC (p), ICC Host: Mouse Monoclonal TU-20 unconjugated
anti-Tubulin, beta 3 (TUBB3) (N-Term) antibody (FITC)TUBB3 Reactivity: Human, Mouse, Rat, Dog, Pig FACS, ICC Host: Mouse Monoclonal TU-20 FITC
anti-Tubulin, beta 3 (TUBB3) (AA 36-63) antibodyTUBB3 Reactivity: Human, Mouse WB, FACS, IHC (p) Host: Rabbit Polyclonal RB21098 unconjugated
anti-Tubulin, beta 3 (TUBB3) antibodyTUBB3 Reactivity: Human, Mouse WB, ELISA, IHC, FACS Host: Mouse Monoclonal 8E3A7 unconjugated
anti-Tubulin, beta 3 (TUBB3) (AA 36-63) antibodyTUBB3 Reactivity: Human, Mouse WB, ELISA, IHC, FACS Host: Rabbit Polyclonal unconjugated
anti-Tubulin, beta 3 (TUBB3) antibodyTUBB3 Reactivity: Human WB, ELISA, FACS, ICC Host: Mouse Monoclonal 2-00E-09 unconjugated
anti-Tubulin, beta 3 (TUBB3) antibodyTUBB3 Reactivity: Human, Mouse, Rat WB, IF Host: Rabbit Polyclonal unconjugated
anti-Tubulin, beta 3 (TUBB3) antibodyTUBB3 Reactivity: Human WB, ELISA, IHC, IF, IP Host: Mouse Monoclonal unconjugated
anti-Tubulin, beta 3 (TUBB3) antibodyTUBB3 Reactivity: Human WB, ELISA, IHC Host: Mouse Monoclonal unconjugated
anti-Tubulin, beta 3 (TUBB3) (AA 1-210) antibodyTUBB3 Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
-
- Application Notes
-
WB: 1 : 1000 up to 1 : 10000 (AP staining)
ICC: 1 : 1000 up to 1 : 5000
IHC: 1 : 200 up to 1 : 500 - Restrictions
- For Research Use only
-
- by
- Kinexus Bioinformatics Corporation
- No.
- #029822
- Date
- 10/28/2014
- Antigen
- Lot Number
- 302302/3
- Method validated
- Western Blotting
- Positive Control
- Mouse brain
- Negative Control
- Mouse heart, HeLa cells, A431 cells
- Notes
- A band was observed in the positive controls at the expected size (~50 kDa), which is lower in the negative controls.
- Primary Antibody
- Antigen: Tubulin, Beta, 3 (TUBB3) (AA 443-450)
- Catalog number: ABIN1742553
- Lot number: 302302/3
- Dilution: 1:1000
- Secondary Antibody
- Antibody: Donkey anti-Rabbit IgG Antibody (HRP)
- Lot number: F0613
- Dilution: 1:10,000
- Full Protocol
- 1. Cell/tissue total protein lysates were boiled in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol at 95°C for 5 minutes prior to loading.
- 2. 15 μg of boiled lysate were loaded and resolved on a 12% SDS-polyacrylamide gel.
- 3. The Precision Plus Protein™ All Blue Prestained Standards from BioRad (161-0373) were used as molecular mass markers.
- 4. Proteins were transferred onto nitrocellulose membrane by tank transfer and protein transfer was confirmed with Ponceau S staining.
- 5. The immunoblot membrane was blocked in 2.5% skim milk and 1.5% BSA solution in TTBS at room temperature for 60 minutes.
- 6. The membrane was washed in TTBS twice for 5 minutes each.
- 7. The membrane was immersed with the protein side up in the antibody solution in TBS and incubated overnight at 4°C with gentle agitation.
- 8. The membrane was rinsed twice with TTBS.
- 9. The membrane was washed in TTBS twice for 5 minutes each.
- 10. The membrane was washed in TTBS once for 15 minutes.
- 11. The membrane was incubated in the HRP-conjugated secondary antibody solution in TBS for 60 minutes at room temperature with gentle agitation.
- 12. The membrane was rinsed twice with TTBS.
- 13. The membrane was washed in TTBS twice for 5 minutes each.
- 14. The membrane was washed in TTBS once for 15 minutes.
- 15. Signals were detected by chemiluminescence (ECL). The blot was scanned for 320 seconds.
- 16. The membrane was rinsed three times with TTBS.
- 17. Repeated Steps 4-15 with the loading control antibody and its matching secondary antibody. The blot was scanned for 160 seconds.
- Experimental Notes
- - No experimental challenges noted.
Validation #029822 (Western Blotting)!['Independent Validation' Badge]( "Successfully validated")
!['Independent Validation' Badge]( "Successfully validated")
Validation Images![Figure 1: Western Blot for Tubulin, Beta, 3 (TUBB3). Grey arrowhead indicates the expected molecular weight of ~50 kDa.]()
Figure 1: Western Blot for Tubulin, Beta, 3 (TUBB3). Grey arrowhead indicates the expected molecular weight of ~50 kDa.
Full Methods -
- Format
- Liquid
- Buffer
- PBS
- Handling Advice
-
Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable. - Storage
- 4 °C/-20 °C
- Storage Comment
- Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
-
-
: "Neuron-astrocyte interaction enhance GABAergic synaptic transmission in a manner dependent on key metabolic enzymes." in: Frontiers in cellular neuroscience, Vol. 9, pp. 120, (2015) (PubMed).
-
: "Neuron-astrocyte interaction enhance GABAergic synaptic transmission in a manner dependent on key metabolic enzymes." in: Frontiers in cellular neuroscience, Vol. 9, pp. 120, (2015) (PubMed).
-
- Target
- TUBB3 (Tubulin, beta 3 (TUBB3))
- Alternative Name
- beta3-Tubulin (TUBB3) (TUBB3 Products)
- Background
- Synonyms: TuJ1
- Pathways
- Microtubule Dynamics, M Phase
-
