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NSG1 antibody (N-Term)

Verified D4S234E Reactivity: Mouse, Rat WB, ELISA, ICC Host: Goat Polyclonal unconjugated
Catalog No. ABIN5539461
  • Target See all NSG1 (D4S234E) Antibodies
    NSG1 (D4S234E) (Neuron-Specific Protein Family Member 1 (D4S234E))
    Binding Specificity
    • 15
    • 9
    • 8
    • 6
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    • 30
    • 26
    • 16
    • 11
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Mouse, Rat
    Host
    • 42
    • 3
    Goat
    Clonality
    • 42
    • 3
    Polyclonal
    Conjugate
    • 19
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This NSG1 antibody is un-conjugated
    Application
    • 45
    • 28
    • 13
    • 13
    • 6
    • 6
    • 3
    • 3
    • 2
    • 1
    Western Blotting (WB), ELISA, Immunocytochemistry (ICC)
    Purpose
    Nsg1 / Neep21
    Sequence
    EKGTKQPLLE DGFD
    Cross-Reactivity
    Cow, Human, Mouse, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Isotype
    IgG
    Top Product
    Discover our top product D4S234E Primary Antibody
  • Application Notes

    Western Blot: Approx 22 kDa band observed in Mouse fetal Brain lysates (calculated MW of 20.9 kDa according to NP_035072.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour.

    Peptide ELISA: antibody detection limit dilution 1:128000.

    Comment

    Immunocytochemistry: Primary DIV9 cells from Hippocampus E18 Rat embryos, stained for neuronal marker MAP2 in blue and for glial marker GFAP in green showed exclusive labelling of EB11637 (red) in the neurons only. Recommended concentrat

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Digilio, Yap, Winckler et al.: "Ctip2-, Satb2-, Prox1-, and GAD65-Expressing Neurons in Rat Cultures: Preponderance of Single- and Double-Positive Cells, and Cell Type-Specific Expression of Neuron-Specific Gene Family Members, ..." in: PLoS ONE, Vol. 10, Issue 10, pp. e0140010, (2015) (PubMed).

  • Target
    NSG1 (D4S234E) (Neuron-Specific Protein Family Member 1 (D4S234E))
    Alternative Name
    Nsg1 (D4S234E Products)
    Synonyms
    D4S234 antibody, D4S234E antibody, NEEP21 antibody, P21 antibody, Neep21 antibody, m234 antibody, p21 antibody, Bsmrb antibody, PEP1 antibody, neuronal vesicle trafficking associated 1 antibody, neuron specific gene family member 1 antibody, NSG1 antibody, Nsg1 antibody
    Background
    Nsg1, neuron specific gene family member 1, Bsmrb, Neep21, PEP1, brain neuron cytoplasmic protein 1/2, brain specific mRNA b, neuron-specific protein family member 1
    Gene ID
    27065, 18196, 25247
    NCBI Accession
    NP_077042
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