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SMO antibody (AA 551-650)

Name: SMO antibody (AA 551-650)
SKU: ABIN680908
Price: 384.62 USD
Availability: InStock

SMO Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p), IF (p) Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Smoothened/SMO Polyclonal Antibody, Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

IHC (p)

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Smoothened/SMO Polyclonal Antibody, Unconjugated (ABIN680908) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin-embedded rat brain labeled with Anti-Smoothened/SMO Polyclonal Antibody, Unconjugated (ABIN680908) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C.

L1 rat kidney lysates L2 rat brain lysates probed with Anti Smoothened/SMO Polyclonal Antibody, Unconjugated (ABIN680908) at 1:200 overnight at 4 °C.

SDS

4 images

(3 references)

Catalog No. ABIN680908

Datasheet as PDF

Target See all SMO Antibodies

SMO (Smoothened Homolog (Drosophila) (SMO))
Binding Specificity
14

8

7

6

6

6

6

2

2

1

1

1

1

1

1

1

1

1
AA 551-650
Reactivity
52

25

22

2

2

2

2

2

2

2

1

1

1
Human, Mouse, Rat
Host
48

4
Rabbit
Compatible Secondaries
Clonality
49

3
Polyclonal
Conjugate
24

5

4

4

2

2

1

1

1

1

1

1

1

1

1

1

1
This SMO antibody is un-conjugated
Application
44

30

12

9

7

5

2

2

1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity

Human, Mouse, Rat

Predicted Reactivity

Dog,Cow,Pig,Horse,Chicken,Rabbit

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human Smoothened

Isotype

IgG
anti-Smoothened Homolog (Drosophila) (SMO) (AA 554-787) antibody
SMO Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated

anti-Smoothened Homolog (Drosophila) (SMO) (AA 28-233) antibody
SMO Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Smoothened Homolog (Drosophila) (SMO) (N-Term) antibody
SMO Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Smoothened Homolog (Drosophila) (SMO) (N-Term) antibody
SMO Reactivity: Human, Mouse, Rat, Dog, Monkey, Pig, Rabbit, Cow, Hamster, Horse, Bat WB, IHC, IHC (p), IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Smoothened Homolog (Drosophila) (SMO) (1st Extracellular Domain) antibody
SMO Reactivity: Human, Mouse IHC, IHC (p), IF Host: Rabbit Polyclonal unconjugated

anti-Smoothened Homolog (Drosophila) (SMO) (AA 68-117) antibody
SMO Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Smoothened Homolog (Drosophila) (SMO) (AA 653-787) antibody
SMO Reactivity: Human WB, ELISA Host: Mouse Monoclonal 1D9 unconjugated

anti-Smoothened Homolog (Drosophila) (SMO) antibody
SMO Reactivity: Human WB, ELISA, IHC, IHC (p) Host: Mouse Monoclonal 3D10 unconjugated

anti-Smoothened Homolog (Drosophila) (SMO) (N-Term) antibody
SMO Reactivity: Human, Mouse, Rat WB, IHC (p), EIA Host: Rabbit Polyclonal unconjugated

anti-Smoothened Homolog (Drosophila) (SMO) (AA 56-155) antibody
SMO Reactivity: Human ELISA Host: Mouse Monoclonal 3G8 unconjugated

Application Notes

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IF(IHC-P) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Zhou, Zhou, Liu, Shen: "GDC-0449 improves the antitumor activity of nano-doxorubicin in pancreatic cancer in a fibroblast-enriched microenvironment." in: Scientific reports, Vol. 7, Issue 1, pp. 13379, (2019) (PubMed).

Wang, Zhang, Bai, Wang, Du: "Baicalin Attenuates Alcoholic Liver Injury through Modulation of Hepatic Oxidative Stress, Inflammation and Sonic Hedgehog Pathway in Rats." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 39, Issue 3, pp. 1129-40, (2016) (PubMed).
Target

SMO (Smoothened Homolog (Drosophila) (SMO))

Alternative Name

Smoothened homolog (SMO Products)

Synonyms

FZD11 antibody, Gx antibody, SMOH antibody, Smoh antibody, E130215L21Rik antibody, bnb antibody, smoothened antibody, XSmo antibody, smoothened, frizzled class receptor antibody, smoothened, frizzled class receptor L homeolog antibody, SMO antibody, Smo antibody, smo.L antibody, smo antibody

Background

Synonyms: Gx, SMOH, FZD11, Smoothened homolog, SMO, Protein Gx
Background: G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7 and GLI3 in the cilia.

Gene ID

6608

UniProt

Q99835

Pathways

Hedgehog Signaling