NUP153 antibody (AA 610-1191)
(2 references)-
- Target See all NUP153 Antibodies
- NUP153 (Nucleoporin 153kDa (NUP153))
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Binding Specificity
- AA 610-1191
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Reactivity
- Rat
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Host
- Rat
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Clonality
- Monoclonal
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Conjugate
- This NUP153 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
- Specificity
- Specific to human, mouse, rat and monkey Nup153 proteins.
- Cross-Reactivity (Details)
- Not tested for other species
- Characteristics
- When injected into the nucleus,R4C8 accumulates into the nuclear pores of Hela cells. R4C8 works in immuno-cytochemistry very well.
- Purification
- Purified
- Sterility
- Sterile filtered
- Immunogen
- Recombinant GST-fused rat Nup153 (610-1191aa, Zn finger and FG repeats domain)
- Clone
- R4C8
- Isotype
- IgG2a kappa
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anti-Nucleoporin 153kDa (NUP153) (AA 1456-1475) antibody
NUP153 Reactivity: Human, Rat, Xenopus laevis WB, IP, IF, ICC Host: Sheep Polyclonal unconjugated
anti-Nucleoporin 153kDa (NUP153) antibodyNUP153 Reactivity: Human ELISA, IF, IHC Host: Rabbit Monoclonal 8D4 unconjugated Recombinant Antibody
anti-Nucleoporin 153kDa (NUP153) (AA 58-86), (N-Term) antibodyNUP153 Reactivity: Human WB, FACS Host: Rabbit Polyclonal RB17477 unconjugated
anti-Nucleoporin 153kDa (NUP153) antibodyNUP153 Reactivity: Rat WB, ELISA, IF, ICC Host: Rat Monoclonal R4C8 unconjugated
anti-Nucleoporin 153kDa (NUP153) (AA 1238-1468) antibodyNUP153 Reactivity: Human WB, IHC, ICC Host: Rabbit Polyclonal unconjugated
anti-Nucleoporin 153kDa (NUP153) (AA 657-880) antibodyNUP153 Reactivity: Human ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-Nucleoporin 153kDa (NUP153) (C-Term) antibodyNUP153 Reactivity: Human, Rat, Mouse WB, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Nucleoporin 153kDa (NUP153) (AA 1127-1176) antibodyNUP153 Reactivity: Human, Monkey WB Host: Rabbit Polyclonal unconjugated
anti-Nucleoporin 153kDa (NUP153) (AA 65-94), (N-Term) antibodyNUP153 Reactivity: Human WB, FACS, EIA Host: Rabbit Polyclonal unconjugated
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- Application Notes
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1. Western blotting: 160 kDa single band in Hela cell extract
2. Immunocytochemistry
3. ELISA
Other applications have not been tested.
Additional comments:
When injected into the nucleus, R4C8 accumulates into the nuclear pores of Hela cells.
R4C8 works in immuno-cytochemistry very well. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS, 50 % glycerol
- Preservative
- Azide free
- Storage
- -20 °C
- Storage Comment
- Upon receipt aliquot and store at -20 C
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: "Nanomechanical basis of selective gating by the nuclear pore complex." in: Science (New York, N.Y.), Vol. 318, Issue 5850, pp. 640-3, (2007) (PubMed).
: "The nucleoporin nup153 plays a critical role in multiple types of nuclear export." in: Molecular biology of the cell, Vol. 10, Issue 3, pp. 649-64, (1999) (PubMed).
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: "Nanomechanical basis of selective gating by the nuclear pore complex." in: Science (New York, N.Y.), Vol. 318, Issue 5850, pp. 640-3, (2007) (PubMed).
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- Target
- NUP153 (Nucleoporin 153kDa (NUP153))
- Alternative Name
- Nup153 (NUP153 Products)
- Synonyms
- CG4453 antibody, Dmel\\CG4453 antibody, NUP153 antibody, Nup 153 antibody, dNup153 antibody, dmNup153 antibody, nup153 antibody, NUCZINK antibody, B130015D15Rik antibody, C88147 antibody, HNUP153 antibody, N153 antibody, Nucleoporin 153kD antibody, nucleoporin 153 antibody, nucleoporin 153kDa L homeolog antibody, Nup153 antibody, NUP153 antibody, nup153.L antibody
- Background
- The nuclear pore complex (NPC) regulates cargo transport between the cytoplasm and the nucleus. Nup (Nucleoporin) 153 is a large (153 kDa) O-linked glycoprotein which is a component of the basket structure located on the nucleoplasmic face of NPC. Nup153 plays a critical role in nuclear export of RNA and proteins. The antibody was purified from the serum-free cultured medium of the hybridoma under mild conditions by propriety chromatography processes.
- UniProt
- P49790
- Pathways
- Protein targeting to Nucleus
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