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Nephronectin antibody (AA 418-565)

NPNT Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN7436474
  • Target See all Nephronectin (NPNT) Antibodies
    Nephronectin (NPNT)
    Binding Specificity
    • 7
    • 6
    • 6
    • 6
    • 3
    • 2
    • 1
    • 1
    • 1
    AA 418-565
    Reactivity
    • 24
    • 6
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Host
    • 26
    Rabbit
    Clonality
    • 26
    Polyclonal
    Conjugate
    • 11
    • 6
    • 3
    • 2
    • 2
    • 2
    This Nephronectin antibody is un-conjugated
    Application
    • 25
    • 16
    • 6
    • 3
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
    Purpose
    Polyclonal Antibody to Nephronectin (NPNT)
    Specificity
    The antibody is a rabbit polyclonal antibody raised against NPNT. It has been selected for its ability to recognize NPNT in immunohistochemical staining and western blotting.
    Cross-Reactivity
    Mouse
    Purification
    Antigen-specific affinity chromatography followed by Protein A affinity chromatography
    Immunogen
    Recombinant Nephronectin (NPNT) corresdonding to Leu418~Arg565 with N-terminal His Tag
    Isotype
    IgG
  • Application Notes

    Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
    Expiry Date
    24 months
  • Target
    Nephronectin (NPNT)
    Alternative Name
    Nephronectin (NPNT Products)
    Synonyms
    POEM antibody, si:ch211-202n8.2 antibody, egfl6l antibody, 1110009H02Rik antibody, AA682063 antibody, AI314031 antibody, Nctn antibody, EGFL6L antibody, nephronectin antibody, NPNT antibody, npnt antibody, Npnt antibody
    Background
    EGFL6L, POEM, Preosteoblast EGF-like repeat protein with MAM domain, Protein EGFL6-like
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