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RCHY1 antibody

Name: RCHY1 antibody
Brand: antibodies-online
SKU: ABIN1724723
Price: 467.5 USD
Availability: InStock

RCHY1 Reactivity: Human, Rat WB, ELISA, IHC, FACS, ICC Host: Mouse Monoclonal 1H10 unconjugated

Western blot analysis using Pirh2 mouse mAb against Hela (1), A549 (2), MCF-7 (3) and PC-12 (4) cell lysate.

Flow cytometric analysis of PC-12 cells using anti-Pirh2 mAb (blue) and negative control (red).

FACS

Immunofluorescence analysis of Hela cells using Pirh2 mouse mAb (green).

Immunohistochemical analysis of paraffin-embedded human Tonsil tissues using anti-Pirh2 mouse mAb

IHC

4 images

(2 references)

Catalog No. ABIN1724723

Datasheet as PDF

Target See all RCHY1 Antibodies

RCHY1 (Ring Finger and CHY Zinc Finger Domain Containing 1, E3 Ubiquitin Protein Ligase (RCHY1))
Reactivity
35

19

11

2

2

2

2

2

1

1
Human, Rat
Host
25

10
Mouse
Compatible Secondaries
Clonality
24

11
Monoclonal
Conjugate
14

2

2

2

2

2

2

2

1

1

1

1

1

1

1
This RCHY1 antibody is un-conjugated
Application
28

13

13

8

4

3

3

2

2

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Purification

purified

Immunogen

Purified recombinant fragment of human Pirh2 expressed in E. coli.

Clone

1H10

Isotype

IgG1
anti-Ring Finger and CHY Zinc Finger Domain Containing 1, E3 Ubiquitin Protein Ligase (RCHY1) (AA 1-261) antibody
RCHY1 Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-Ring Finger and CHY Zinc Finger Domain Containing 1, E3 Ubiquitin Protein Ligase (RCHY1) antibody
RCHY1 Reactivity: Human, Mouse WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-Ring Finger and CHY Zinc Finger Domain Containing 1, E3 Ubiquitin Protein Ligase (RCHY1) (AA 1-252) antibody
RCHY1 Reactivity: Human WB Host: Rabbit Polyclonal unconjugated

anti-Ring Finger and CHY Zinc Finger Domain Containing 1, E3 Ubiquitin Protein Ligase (RCHY1) (AA 31-130) antibody
RCHY1 Reactivity: Human, Mouse WB, ELISA, IF (p), IF (cc), IHC (p), IHC (fro), ICC Host: Rabbit Polyclonal unconjugated

anti-Ring Finger and CHY Zinc Finger Domain Containing 1, E3 Ubiquitin Protein Ligase (RCHY1) (AA 1-100) antibody
RCHY1 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 5D12 unconjugated

anti-Ring Finger and CHY Zinc Finger Domain Containing 1, E3 Ubiquitin Protein Ligase (RCHY1) (AA 36-85) antibody
RCHY1 Reactivity: Human, Rat, Sheep, Cow, Horse, Rabbit, Monkey WB Host: Rabbit Polyclonal unconjugated

anti-Ring Finger and CHY Zinc Finger Domain Containing 1, E3 Ubiquitin Protein Ligase (RCHY1) (AA 31-130) antibody (Biotin)
RCHY1 Reactivity: Human, Mouse WB, ELISA, IHC (p), IHC (fro) Host: Rabbit Polyclonal Biotin

anti-Ring Finger and CHY Zinc Finger Domain Containing 1, E3 Ubiquitin Protein Ligase (RCHY1) (AA 31-130) antibody (Alexa Fluor 350)
RCHY1 Reactivity: Human, Mouse WB, IF (p), IF (cc) Host: Rabbit Polyclonal Alexa Fluor 350

anti-Ring Finger and CHY Zinc Finger Domain Containing 1, E3 Ubiquitin Protein Ligase (RCHY1) (AA 31-130) antibody (Alexa Fluor 488)
RCHY1 Reactivity: Human, Mouse WB, IF (p), IF (cc) Host: Rabbit Polyclonal Alexa Fluor 488

anti-Ring Finger and CHY Zinc Finger Domain Containing 1, E3 Ubiquitin Protein Ligase (RCHY1) (AA 31-130) antibody (Alexa Fluor 555)
RCHY1 Reactivity: Human, Mouse WB, IF (p), IF (cc) Host: Rabbit Polyclonal Alexa Fluor 555

Application Notes

ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, ICC: 1:200 - 1:1000, FCM: 1:200 - 1:400

Restrictions

For Research Use only
Format

Liquid

Buffer

Ascitic fluid containing 0.03 % sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C/-20 °C

Storage Comment

4°C, -20°C for long term storage
Duan, Gao, Wu, Zhang, Otterson, Villalona-Calero: "Differential response between the p53 ubiquitin-protein ligases Pirh2 and MdM2 following DNA damage in human cancer cells." in: Experimental cell research, Vol. 312, Issue 17, pp. 3370-8, (2006) (PubMed).

Duan, Gao, Druhan, Zhu, Morrison, Otterson, Villalona-Calero: "Expression of Pirh2, a newly identified ubiquitin protein ligase, in lung cancer." in: Journal of the National Cancer Institute, Vol. 96, Issue 22, pp. 1718-21, (2004) (PubMed).
Target

RCHY1 (Ring Finger and CHY Zinc Finger Domain Containing 1, E3 Ubiquitin Protein Ligase (RCHY1))

Alternative Name

Pirh2 (RCHY1 Products)

Synonyms

ARNIP antibody, CHIMP antibody, PIRH2 antibody, PRO1996 antibody, RNF199 antibody, ZNF363 antibody, Pirh2 antibody, Zfp363 antibody, Znf363 antibody, wu:fb37e07 antibody, zgc:101128 antibody, RCHY1 antibody, arnip antibody, chimp antibody, pirh2 antibody, harnip antibody, rnf199 antibody, znf363 antibody, pro1996 antibody, ring finger and CHY zinc finger domain containing 1 antibody, ring finger and CHY zinc finger domain containing 1 L homeolog antibody, RCHY1 antibody, Rchy1 antibody, rchy1 antibody, rchy1.L antibody

Background

Description: Pirh 2 (P53 induced RING-H2 protein), also known as RCHY1, it forms dimers through its N- and C-terminus in cells. The Pirh2 has ubiquitin-protein ligase activity and it binds with p53 and promotes the ubiquitin-mediated proteosomal degradation of p53. The Pirh2 is oncogenic because loss of p53 function contributes directly to malignant tumor development. Pirh2 expression decreases the level of p53, and a decrease of endogenous Pirh2 expression increases p53 levels. Pirh2 is therefore considered, together with MDM2, to act as a negative regulator of p53 function.
Aliases: ARNIP, CHIMP, RNF199, RCHY1

Molecular Weight

30 kDa, 60 kDa (homodimer)

Gene ID

25898

HGNC

25898