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PTPN22 antibody (Internal Region)

Verified PTPN22 Reactivity: Human ELISA, IHC, IF, FACS Host: Goat Polyclonal unconjugated
Catalog No. ABIN6391380
  • Target See all PTPN22 Antibodies
    PTPN22 (Tyrosine-Protein Phosphatase Non-Receptor Type 22 (PTPN22))
    Binding Specificity
    • 13
    • 9
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 43
    • 15
    • 14
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 39
    • 6
    Goat
    Clonality
    • 41
    • 4
    Polyclonal
    Conjugate
    • 25
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This PTPN22 antibody is un-conjugated
    Application
    • 32
    • 27
    • 7
    • 6
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
    Purpose
    LYP / PTPN22
    Sequence
    CPPNKPAESV QSNNS
    Specificity
    This antibody is specific for human LYP1 and will not cross-react with LYP2.
    Cross-Reactivity
    Chimpanzee, Human
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Isotype
    IgG
  • Application Notes

    Immunohistochemistry: Paraffin embedded Human Small Intestine, Thyroid and Adrenal Gland. Recommended concentration: 3.75 μg/mL.

    Western Blot: Preliminary experiments showed a 100 kDa band in some Daudi cell lysates at 0.5-1 μg/mL. This molecular weight is routinely observed by other sources. An additional band at 75 kDa was also observed and both bands were successfully blocked by i

    Peptide ELISA: antibody detection limit dilution 1:32000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    PTPN22 (Tyrosine-Protein Phosphatase Non-Receptor Type 22 (PTPN22))
    Alternative Name
    PTPN22 (PTPN22 Products)
    Synonyms
    70zpep antibody, PEP antibody, Ptpn8 antibody, LYP antibody, LYP1 antibody, LYP2 antibody, PTPN8 antibody, protein tyrosine phosphatase, non-receptor type 22 (lymphoid) antibody, protein tyrosine phosphatase, non-receptor type 22 antibody, Ptpn22 antibody, PTPN22 antibody
    Background
    PTPN22, LYP, Lyp1, Lyp2, protein tyrosine phosphatase, non-receptor type 22 (lymphoid), protein tyrosine phosphatase homolog, lymphoid-specific protein tyrosine phosphatase
    Gene ID
    26191
    NCBI Accession
    NP_057051, NP_036543, NP_001180360, NP_001295226
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