For best experience we recommend to activate Javascript in your browser.

JNK antibody (pThr183, pTyr185)

Name: JNK antibody (pThr183, pTyr185)
SKU: ABIN732368
Price: 384.62 USD
Availability: InStock

MAPK8 Reactivity: Human, Mouse, Rat, Dog, Rabbit WB, ELISA, IHC (p), FACS, IF (cc), IF (p), ICC, IHC (fro) Host: Rabbit Polyclonal unconjugated

L1 mouse brain lysates L2 human colon carcinoma lysates probed with Anti - phospho-JNK1/2/3 (Thr183+Tyr185) Polyclonal Antibody, Unconjugated (ABIN732368) at 1:200 in 4 °C.

SDS

L1 mouse brain lysates L2 human colon carcinoma lysates probed with Anti - phospho-JNK1/2/3 (Thr183+Thr185) Polyclonal Antibody, Unconjugated at 1:200 in 4 ˚C.

SDS

Mouse splenocytes probed with Rabbit Anti-JNK1/2/3 (Thr183+Tyr185) Polyclonal Antibody, Unconjugated .

FACS

4 images

(16 references)

Catalog No. ABIN732368

Datasheet as PDF

Target See all JNK (MAPK8) Antibodies

JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))
Binding Specificity
56

27

17

16

16

10

9

9

8

8

8

6

6

6

6

6

5

4

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pThr183, pTyr185
Reactivity
207

113

98

43

33

20

17

9

5

4

2

2

1

1

1

1
Human, Mouse, Rat, Dog, Rabbit
Host
194

18

3
Rabbit
Compatible Secondaries
Clonality
179

36
Polyclonal
Conjugate
100

18

16

13

12

10

5

5

5

5

5

5

3

3

3

3

3

1
This JNK antibody is un-conjugated
Application
162

92

54

42

39

38

36

30

20

18

13

11

10

3

2

2

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity

Dog, Human, Mouse, Rabbit, Rat

Predicted Reactivity

Cow,Pig

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic phosphopeptide derived from human JNK1 around the phosphorylation site of Thr183/Tyr185

Isotype

IgG

Top Product

Discover our top product MAPK8 Primary Antibody
anti-Mitogen-Activated Protein Kinase 8 (MAPK8) (AA 245-345) antibody
MAPK8 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 8 (MAPK8) antibody
MAPK8 Reactivity: Human, Mouse, Rat, Pig WB, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 8 (MAPK8) (AA 1-384) antibody
MAPK8 Reactivity: Human WB, ELISA, IF, PLA, RNAi Host: Mouse Monoclonal 2F11 unconjugated

anti-Mitogen-Activated Protein Kinase 8 (MAPK8) (AA 201-300) antibody
MAPK8 Reactivity: Human, Mouse, Rat, Chicken, Dog WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 8 (MAPK8) (AA 1-427) antibody
MAPK8 Reactivity: Human WB, IF, PLA Host: Rabbit Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 8 (MAPK8) (AA 318-427) antibody
MAPK8 Reactivity: Human WB, ELISA, IF, IP Host: Mouse Monoclonal 4A11 unconjugated

anti-Mitogen-Activated Protein Kinase 8 (MAPK8) (pThr183) antibody
MAPK8 Reactivity: Human, Mouse, Rat, Chicken WB, ELISA, IF, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 8 (MAPK8) (AA 5-300) antibody
MAPK8 Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 8 (MAPK8) (AA 151-250) antibody
MAPK8 Reactivity: Human, Mouse, Rat, Chicken, Pig WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 8 (MAPK8) (AA 358-389), (C-Term) antibody
MAPK8 Reactivity: Human WB, IF, IHC (p) Host: Rabbit Polyclonal RB0922 unconjugated

Application Notes

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Uehara, Temma, Kobayashi, Irie, Yamaguchi: "Reduction of Thermotolerance by Heat Shock Protein 90 Inhibitors in Murine Erythroleukemia Cells." in: Biological & pharmaceutical bulletin, Vol. 41, Issue 9, pp. 1393-1400, (2018) (PubMed).

Ning, Wang, Gao, Mu, Wang, Liu, Zhu, Meng: "Chicory inulin ameliorates type 2 diabetes mellitus and suppresses JNK and MAPK pathways in vivo and in vitro." in: Molecular nutrition & food research, (2017) (PubMed).

Liu, Zhang, Han, Wang, Liu, Zhang, Zhou, Xiang: "Inhibition of BTK protects lungs from trauma-hemorrhagic shock-induced injury in rats." in: Molecular medicine reports, Vol. 16, Issue 1, pp. 192-200, (2017) (PubMed).

Li, Wang, Zhou, Zhang, Le, He: "Downregulation of BRAF-activated non-coding RNA suppresses the proliferation, migration and invasion, and induces apoptosis of hepatocellular carcinoma cells." in: Oncology letters, Vol. 14, Issue 4, pp. 4751-4757, (2017) (PubMed).

Cong, Chen: "Neuroprotective Effect of Ginsenoside Rd in Spinal Cord Injury Rats." in: Basic & clinical pharmacology & toxicology, Vol. 119, Issue 2, pp. 193-201, (2016) (PubMed).

Zhang, Lin, Jin, Lin, Cao, Zheng: "Overexpression of WNT5B promotes COLO 205 cell migration and invasion through the JNK signaling pathway." in: Oncology reports, Vol. 36, Issue 1, pp. 23-30, (2016) (PubMed).

Iriyama, Hatta, Takei: "Direct effect of dasatinib on signal transduction pathways associated with a rapid mobilization of cytotoxic lymphocytes." in: Cancer medicine, Vol. 5, Issue 11, pp. 3223-3234, (2016) (PubMed).

Yu, Shi, Qi, Zhao, Gao, Li: "Gypenoside Protects Cardiomyocytes against Ischemia-Reperfusion Injury via the Inhibition of Mitogen-Activated Protein Kinase Mediated Nuclear Factor Kappa B Pathway In Vitro and In Vivo." in: Frontiers in pharmacology, Vol. 7, pp. 148, (2016) (PubMed).

Zhao, Xue, Guo, Sun, Liu, Wang: "Inhibition of endocan attenuates monocrotaline-induced connective tissue disease related pulmonary arterial hypertension." in: International immunopharmacology, Vol. 42, pp. 115-121, (2016) (PubMed).

Zhao, Liu, Liu, Han, Zhao: "Betulin attenuates lung and liver injuries in sepsis." in: International immunopharmacology, Vol. 30, pp. 50-6, (2015) (PubMed).

Xing, He, Yu, Hou, Chen: "Increased expression of receptor for advanced glycation end-products worsens focal brain ischemia in diabetic rats." in: Neural regeneration research, Vol. 7, Issue 13, pp. 1000-5, (2015) (PubMed).

Król, Mucha, Majchrzak, Homa, Bulkowska, Majewska, Gajewska, Pietrzak, Perszko, Romanowska, Paw?owski, Manuali, Hellmen, Motyl: "Macrophages mediate a switch between canonical and non-canonical Wnt pathways in canine mammary tumors." in: PLoS ONE, Vol. 9, Issue 1, pp. e83995, (2014) (PubMed).

Rosenzweig, Ou, Quinn: "P38 mitogen-activated protein kinase promotes dedifferentiation of primary articular chondrocytes in monolayer culture." in: Journal of cellular and molecular medicine, Vol. 17, Issue 4, pp. 508-17, (2013) (PubMed).

Song, Liu, Li, Liu, Song, Song: "Protective effects of lipoic acid and mesna on cyclophosphamide-induced haemorrhagic cystitis in mice." in: Cell biochemistry and function, (2013) (PubMed).

Rosenzweig, Djap, Ou, Quinn: "Mechanical injury of bovine cartilage explants induces depth-dependent, transient changes in MAP kinase activity associated with apoptosis." in: Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society, Vol. 20, Issue 12, pp. 1591-602, (2012) (PubMed).
Target

JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Alternative Name

JNK1+2+3 (MAPK8 Products)

Synonyms

JNK antibody, JNK-46 antibody, JNK1 antibody, JNK1A2 antibody, JNK21B1/2 antibody, PRKM8 antibody, SAPK1 antibody, SAPK1c antibody, AI849689 antibody, Prkm8 antibody, BSK antibody, BSK/DJNK antibody, Bsk antibody, CG5680 antibody, D-JNK antibody, D-junk antibody, DBSK/JNK antibody, DJNK antibody, DJNK/bsk antibody, Dmel\\CG5680 antibody, JNK/SAPK antibody, Jnk antibody, Junk antibody, SAPKa antibody, c-Jun antibody, dJNK antibody, jnk antibody, T10F20.15 antibody, jnk1 antibody, sapk1 antibody, mapk8 antibody, zgc:112379 antibody, JNKb antibody, mapk8b antibody, mitogen-activated protein kinase 8 antibody, Stress-activated protein kinase jnk-1 antibody, basket antibody, Protein kinase superfamily protein antibody, mitogen-activated protein kinase 8 L homeolog antibody, mitogen-activated protein kinase 8b antibody, mitogen-activated protein kinase 8B-like antibody, MAPK8 antibody, jnk-1 antibody, Mapk8 antibody, bsk antibody, ATMPK8 antibody, mapk8.L antibody, mapk8b antibody, LOC109094263 antibody

Background

Synonyms: JNK, JNK1, PRKM8, SAPK1, JNK-46, JNK1A2, SAPK1c, JNK21B1/2, Mitogen-activated protein kinase 8, MAP kinase 8, MAPK 8, Stress-activated protein kinase 1c, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, MAPK8
Background: JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.

Gene ID

5599

UniProt

P45983

Pathways

MAPK Signaling, WNT Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, S100 Proteins