Cullin 1 antibody
-
- Target See all Cullin 1 (CUL1) Antibodies
- Cullin 1 (CUL1)
-
Reactivity
- Human, Mouse
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This Cullin 1 antibody is un-conjugated
-
Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
- Specificity
- Cullin 1 antibody detects endogenous levels of total Cullin 1
- Cross-Reactivity
- Human, Mouse (Murine)
- Purification
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- Immunogen
- A synthesized peptide derived from human Cullin 1
- Isotype
- IgG
- Top Product
- Discover our top product CUL1 Primary Antibody
-
anti-Cullin 1 (CUL1) (AA 500-776) antibody
CUL1 Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated
anti-Cullin 1 (CUL1) antibodyCUL1 Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Cullin 1 (CUL1) (AA 1-250) antibodyCUL1 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Cullin 1 (CUL1) antibodyCUL1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Cullin 1 (CUL1) (AA 1-776) antibodyCUL1 Reactivity: Human WB, IF, PLA Host: Rabbit Polyclonal unconjugated
anti-Cullin 1 (CUL1) (C-Term) antibodyCUL1 Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Cullin 1 (CUL1) (C-Term) antibodyCUL1 Reactivity: Human, Mouse WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Cullin 1 (CUL1) (C-Term) antibodyCUL1 Reactivity: Human WB, ELISA, IHC, IP, IHC (p) Host: Mouse Monoclonal unconjugated
anti-Cullin 1 (CUL1) (C-Term) antibodyCUL1 Reactivity: Human, Mouse, Rat, Chicken, Cow, Dog, Rabbit, Pig WB, IHC, IF, IC Host: Rabbit Polyclonal unconjugated
anti-Cullin 1 (CUL1) (AA 727-776) antibodyCUL1 Reactivity: Human, Mouse WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
-
- Application Notes
- WB: 1:500~1:3000 IHC: 1:50~1:200 IF/ICC: 1:100~1:500
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20 °C.Stable for 12 months from date of receipt
- Expiry Date
- 12 months
-
- Target
- Cullin 1 (CUL1)
- Alternative Name
- Cullin 1 (CUL1 Products)
- Synonyms
- LOC100282292 antibody, Cullin-1 antibody, Cul1 antibody, zgc:55747 antibody, zgc:77500 antibody, wu:fc13a10 antibody, cullin-1 antibody, ATCUL1 antibody, AUXIN RESISTANT 6 antibody, AXR6 antibody, T10P11.26 antibody, T10P11_26 antibody, cullin 1 antibody, cul1 antibody, wu:fi43h07 antibody, wu:fj43a08 antibody, zgc:111899 antibody, zgc:55483 antibody, Cul-1 antibody, cullin 1 antibody, cullin-1 antibody, Cullin 1 antibody, Cullin-1 antibody, cullin 1a antibody, cullin 1 S homeolog antibody, cullin 1b antibody, CUL1 antibody, LOC100282292 antibody, cul1 antibody, ATEG_00491 antibody, EDI_072700 antibody, EDI_264400 antibody, SJAG_02591 antibody, BDBG_03785 antibody, Cul1 antibody, cul1a antibody, cul1.S antibody, cul-1 antibody, cul1b antibody
- Background
-
Description: Core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. SCF complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins (PubMed:27565346). In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and exchange of the substrate recognition component is mediated by TIP120A/CAND1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2. SCF(BTRC) and/or SCF(FBXW11) direct ubiquitination of CEP68 (PubMed:25704143, PubMed:25503564). SCF(SKP2) directs ubiquitination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquitination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO1) directs ubiquitination of BCL6 and DTL but does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22', the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(CCNF) directs ubiquitination of CCP110. SCF(FBXL3) and SCF(FBXL21) direct ubiquitination of CRY1 and CRY2. SCF(FBXO9) directs ubiquitination of TTI1 and TELO2. SCF(FBXO10) directs ubiquitination of BCL2.
Gene: CUL1
- Molecular Weight
- 90kDa
- Gene ID
- 8454
- UniProt
- Q13616
- Pathways
- Cell Division Cycle, Hedgehog Signaling, Mitotic G1-G1/S Phases, Regulation of Cell Size
-
