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GCLM antibody (C-Term)

Name: GCLM antibody (C-Term)
SKU: ABIN653046
Price: 429 USD
Availability: InStock

GCLM Reactivity: Human, Mouse WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB22980 unconjugated

Western blot analysis of GCLM Antibody (C-term) (ABIN653046 and ABIN2842657) in 293 cell line and mouse kidney tissue lysates (35 μg/lane).

IHC (p)

Confocal immunofluorescent analysis of GCLM Antibody (C-term) (ABIN653046 and ABIN2842657) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).

Western blot analysis of GCLM (arrow) using rabbit polyclonal GCLM Antibody (C-term) (ABIN653046 and ABIN2842657).

Anti-GCLM Antibody (C-term) at 1:8000 dilution + Hela whole cell lysate Lysates/proteins at 20 μg per lane.

IHC (p)

GCLM Antibody (C-term) (ABIN653046 and ABIN2842657) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).

FACS

7 images

(1 reference)

Catalog No. ABIN653046

Datasheet as PDF

Target See all GCLM Antibodies

GCLM (Glutamate-Cysteine Ligase, Modifier Subunit (GCLM))
Binding Specificity
11

7

7

7

6

2

1

1

1

1

1

1

1

1
AA 246-274, C-Term
Reactivity
63

33

26

3

3

2

2

2

2

2

2

1
Human, Mouse
Host
57

6
Rabbit
Compatible Secondaries
Clonality
50

13
Polyclonal
Conjugate
29

5

4

3

3

3

3

3

3

3

2

2
This GCLM antibody is un-conjugated
Application
38

28

25

18

15

8

7

5

2

2

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen

This GCLM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 246-274 amino acids from the C-terminal region of human GCLM.

Clone

RB22980

Isotype

Ig Fraction
anti-Glutamate-Cysteine Ligase, Modifier Subunit (GCLM) (AA 40-251) antibody
GCLM Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Glutamate-Cysteine Ligase, Modifier Subunit (GCLM) (AA 1-274), (Regulatory Subunit) antibody
GCLM Reactivity: Human WB, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated

anti-Glutamate-Cysteine Ligase, Modifier Subunit (GCLM) (AA 1-274) antibody
GCLM Reactivity: Human WB, ELISA Host: Mouse Monoclonal 2B8 unconjugated

anti-Glutamate-Cysteine Ligase, Modifier Subunit (GCLM) (AA 245-274), (C-Term) antibody
GCLM Reactivity: Human WB, IF, FACS, IHC (p), EIA Host: Rabbit Polyclonal unconjugated

anti-Glutamate-Cysteine Ligase, Modifier Subunit (GCLM) (N-Term) antibody
GCLM Reactivity: Human, Mouse, Rat, Pig WB, IHC, ELISA Host: Rabbit Polyclonal unconjugated

anti-Glutamate-Cysteine Ligase, Modifier Subunit (GCLM) (AA 1-274) antibody
GCLM Reactivity: Human WB Host: Mouse Polyclonal unconjugated

anti-Glutamate-Cysteine Ligase, Modifier Subunit (GCLM) (AA 1-274) antibody
GCLM Reactivity: Human WB, IP Host: Rabbit Polyclonal unconjugated

anti-Glutamate-Cysteine Ligase, Modifier Subunit (GCLM) (AA 182-231) antibody
GCLM Reactivity: Human, Mouse, Rat, Cow, Monkey, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Pig, Bat WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Glutamate-Cysteine Ligase, Modifier Subunit (GCLM) (AA 1-274) antibody
GCLM Reactivity: Human WB, ELISA, IF, ICC Host: Mouse Monoclonal AT2D12 unconjugated

anti-Glutamate-Cysteine Ligase, Modifier Subunit (GCLM) antibody
GCLM Reactivity: Human, Mouse IHC Host: Rabbit Polyclonal unconjugated

Application Notes

IF: 1:10~50. WB: 1:1000. WB: 1:1000. WB: 1:8000. IHC-P: 1:25. IHC-P: 1:25. FC: 1:10~50

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date

6 months
Chen, Jiang, Hu, Zhang: "The protective role of resveratrol in the sodium arsenite-induced oxidative damage via modulation of intracellular GSH homeostasis." in: Biological trace element research, Vol. 155, Issue 1, pp. 119-31, (2014) (PubMed).
Target

GCLM (Glutamate-Cysteine Ligase, Modifier Subunit (GCLM))

Alternative Name

GCLM (GCLM Products)

Synonyms

Glclr antibody, GLCLR antibody, id:ibd3182 antibody, wu:fi24c07 antibody, zgc:55903 antibody, AI649393 antibody, Gcmc antibody, glutamate-cysteine ligase regulatory subunit antibody, glutamate cysteine ligase, modifier subunit antibody, glutamate-cysteine ligase modifier subunit antibody, glutamate-cysteine ligase, modifier subunit antibody, glutamate-cysteine ligase, modifier subunit L homeolog antibody, PTRG_09814 antibody, Gclm antibody, GCLM antibody, gclm antibody, gclm.L antibody

Background

GCLM known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.

Molecular Weight

30727

Gene ID

2730

NCBI Accession

NP_002052

UniProt

P48507