For best experience we recommend to activate Javascript in your browser.

SKP2 antibody (AA 351-424)

Name: SKP2 antibody (AA 351-424)
SKU: ABIN737601
Price: 384.62 USD
Availability: InStock

SKP2 Reactivity: Human, Mouse, Rat WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin embedded human thyroid carcinoma labeled with Anti-SKP2/skp2 p45 Polyclonal Antibody, Unconjugated (ABIN737601) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

IHC

MCF-7 cells were stained with SKP2 Polyclonal Antibody, Unconjugated at 1:500 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody.

IF (cc)

293T lysates probed with SKP2 Polyclonal Antibody, Unconjugated at 1:500 dilution and 4˚C overnight incubation.

Formalin-fixed and paraffin embedded human thyroid carcinoma labeled with Anti-SKP2/skp2 p45 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.

IHC (p)

Hela cells probed with SKP2 Polyclonal Antibody, Unconjugated (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).

FACS

5 images

(4 references)

Catalog No. ABIN737601

Datasheet as PDF

Target See all SKP2 Antibodies

SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))
Binding Specificity
15

8

7

6

5

2

2

1

1

1

1

1

1

1

1

1

1

1

1
AA 351-424
Reactivity
69

34

32

5

5

2

1

1

1

1
Human, Mouse, Rat
Host
61

9

1
Rabbit
Compatible Secondaries
Clonality
61

9
Polyclonal
Conjugate
40

4

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1
This SKP2 antibody is un-conjugated
Application
49

19

18

16

14

12

12

12

11

6

2

2

1

1

1
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity

Human, Mouse, Rat

Predicted Reactivity

Cow,Rabbit

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human Skp2

Isotype

IgG
anti-S-Phase Kinase-Associated Protein 2 (SKP2) (C-Term) antibody
SKP2 Reactivity: Human, Mouse WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-S-Phase Kinase-Associated Protein 2 (SKP2) (AA 1-100) antibody
SKP2 Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated

anti-S-Phase Kinase-Associated Protein 2 (SKP2) antibody
SKP2 Reactivity: Human, Mouse, Rat WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-S-Phase Kinase-Associated Protein 2 (SKP2) (AA 43-397) antibody
SKP2 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-S-Phase Kinase-Associated Protein 2 (SKP2) (AA 1-424) antibody
SKP2 Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-S-Phase Kinase-Associated Protein 2 (SKP2) antibody
SKP2 Reactivity: Human ELISA, IHC, IF Host: Rabbit Monoclonal 6D4 unconjugated Recombinant Antibody

anti-S-Phase Kinase-Associated Protein 2 (SKP2) antibody
SKP2 Reactivity: Human ELISA, IF, FACS Host: Rabbit Monoclonal 3C11 unconjugated Recombinant Antibody

anti-S-Phase Kinase-Associated Protein 2 (SKP2) (AA 156-185) antibody
SKP2 Reactivity: Human WB, FACS Host: Rabbit Polyclonal RB19860 unconjugated

anti-S-Phase Kinase-Associated Protein 2 (SKP2) (AA 1-130) antibody
SKP2 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 6G9D10 unconjugated

anti-S-Phase Kinase-Associated Protein 2 (SKP2) (AA 1-424) antibody
SKP2 Reactivity: Human WB, PLA Host: Mouse Polyclonal unconjugated

Application Notes

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Pan, Li, Zhong, Chen, Chen, Gao, Wu, Guo: "RIG-I inhibits pancreatic β cell proliferation through competitive binding of activated Src." in: Scientific reports, Vol. 6, pp. 28914, (2016) (PubMed).

Yang, Wang, Wang, Zhang, Zhang, Lu, Wang: "mTOR is involved in 17?-estradiol-induced, cultured immature boar Sertoli cell proliferation via regulating the expression of SKP2, CCND1, and CCNE1." in: Molecular reproduction and development, Vol. 82, Issue 4, pp. 305-14, (2015) (PubMed).

Jiao, Yi, Rong, Kee, Zhong: "MicroRNA-1285 regulates 17?-estradiol-inhibited immature boar Sertoli cell proliferation via adenosine monophosphate-activated protein kinase activation." in: Endocrinology, pp. en20141982, (2015) (PubMed).

Sun, Yang, Luo, Wang, Chen, Zhang, Wang, Li: "Thyroid hormone inhibits the proliferation of piglet Sertoli cell via PI3K signaling pathway." in: Theriogenology, (2014) (PubMed).
Target

SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

Alternative Name

SKP2 (SKP2 Products)

Synonyms

FBXL1 antibody, FBL1 antibody, FLB1 antibody, p45 antibody, RGD1562456 antibody, S-phase kinase-associated protein 2 (p45) antibody, S-phase kinase associated protein 2 antibody, Skp2 antibody, SKP2 antibody

Background

Synonyms: p45, FBL1, FLB1, FBXL1, S-phase kinase-associated protein 2, Cyclin-A/CDK2-associated protein p45, F-box protein Skp2, F-box/LRR-repeat protein 1, p45skp2, SKP2
Background: Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, KMT2A/MLL1, CDK9, RAG2, FOXO1, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2. Promotes ubiquitination and destruction of CDH1 in a CK1-Dependent Manner, thereby regulating cell migration.

Gene ID

6502

UniProt

Q13309

Pathways

Mitotic G1-G1/S Phases