SPG21 Protein (GST tag,His tag)
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- Target See all SPG21 Proteins
- SPG21 (Spastic Paraplegia 21 (SPG21))
- Protein Type
- Recombinant
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Origin
- Mouse
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This SPG21 protein is labelled with GST tag,His tag.
- Purpose
- Recombinant Mouse SPG21 Protein (His & GST Tag)
- Sequence
- Met 1-Pro 308
- Characteristics
- A DNA sequence encoding the mouse SPG21 isoform 1 (Q9CQC8-1) (Met 1-Pro 308) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
- Purity
- > 90 % as determined by SDS-PAGE
- Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Top Product
- Discover our top product SPG21 Protein
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Spastic Paraplegia 21 (SPG21) (AA 1-308) protein (His tag)
SPG21 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS - PAGE SDS
Spastic Paraplegia 21 (SPG21) (AA 1-308) protein (GST tag)SPG21 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Spastic Paraplegia 21 (SPG21) (AA 211-306) protein (GST tag)SPG21 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Spastic Paraplegia 21 (SPG21) (AA 1-308) protein (Strep Tag)Crystallography grade SPG21 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
Spastic Paraplegia 21 (SPG21) (AA 1-308) protein (Strep Tag)Crystallography grade SPG21 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
Spastic Paraplegia 21 (SPG21) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)SPG21 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Spastic Paraplegia 21 (SPG21) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)SPG21 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 3 mM DTT, 10 % glycerol
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- SPG21 (Spastic Paraplegia 21 (SPG21))
- Alternative Name
- SPG21 (SPG21 Products)
- Synonyms
- ACP33 Protein, GL010 Protein, MAST Protein, BM-019 Protein, C78576 Protein, D9Wsu18e Protein, Maspardin Protein, wu:fd07h02 Protein, zgc:73091 Protein, SPG21, maspardin Protein, spastic paraplegia 21 (autosomal recessive, Mast syndrome) Protein, SPG21, maspardin S homeolog Protein, SPG21 Protein, Spg21 Protein, spg21.S Protein, spg21 Protein
- Background
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Background: Spastic paraplegia 21 (SPG21), also known as acid Cluster Protein 33 (ACP33) and Mast syndrome protein, is a member of the AB hydrolase superfamily. Human SPG21 is a 308 amino acid residue protein widely expressed in all tissues, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. SPG21 binds to the hydrophobic C-terminal amino acids of CD4 which are involved in repression of T cell activation via the noncatalytic alpha/beta hydrolase fold domain. SPG21 thus is proposed to play a role as a negative regulatory factor in CD4-dependent T-cell activation of CD4. Defects in SPG21 are the cause of spastic paraplegia autosomal recessive type 21, also known as Mast syndrome, a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. SPG21 is also associated with dementia and other central nervous system abnormalities.
Synonym: ACP33,BM-019,C78576,D9Wsu18e,GL010,MAST
- Molecular Weight
- 62.8kDa
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