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14-3-3 sigma/SFN antibody

Name: 14-3-3 sigma/SFN antibody
Brand: antibodies-online
SKU: ABIN2854867
Price: 609.18 USD
Availability: InStock

SFN Reactivity: Human WB, IF, IHC (p), IP, ICC Host: Rabbit Polyclonal unconjugated

WB Image 14-3-3 sigma antibody detects SFN protein by Western blot analysis.

IP Image 14-3-3 sigma antibody immunoprecipitates SFN protein in IP experiments.

IHC-P Image Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using 14-3-3 sigma, antibody at 1:100 dilution.

IHC

ICC/IF Image 14-3-3 sigma antibody detects 14-3-3 sigma protein at cytoplasm by immunofluorescent analysis.

IHC-P Image 14-3-3 sigma antibody detects 14-3-3 sigma protein at cytosol on mouse skin by immunohistochemical analysis.

IHC

AKT regulates UVB-mediated nuclear translocation of TC45 in keratinocytesA.

AKT cooperates with 14-3-3σ to facilitate nuclear translocation of TC45 in response to UVB irradiationWestern blot analysis of A.

14-3-3σ transports TC45 into the keratinocyte nucleus following UVB irradiation through direct binding3PC keratinocytes overexpressing A.

IF (cc)

18 images

(1 reference)

Catalog No. ABIN2854867

Datasheet as PDF

Target See all 14-3-3 sigma/SFN (SFN) Antibodies

14-3-3 sigma/SFN (SFN) (Stratifin (SFN))
Reactivity
101

35

28

5

5

5

4

4

4

4

3

1

1

1

1

1
Human
Host
91

9

2

1
Rabbit
Compatible Secondaries
Clonality
87

16
Polyclonal
Conjugate
46

11

9

6

4

4

3

3

3

3

3

3

1

1

1

1

1
This 14-3-3 sigma/SFN antibody is un-conjugated
Application
84

44

37

22

16

14

14

13

13

8

3

2

1

1

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Cross-Reactivity

Human, Mouse

Characteristics

Rabbit Polyclonal antibody to 14-3-3 sigma (stratifin)
14-3-3 sigma antibody

Purification

Purified by antigen-affinity chromatography.

Immunogen

Recombinant protein encompassing a sequence within the center region of human 14-3-3 sigma. The exact sequence is proprietary.

Isotype

IgG
anti-Stratifin (SFN) (Internal Region) antibody
Verified SFN Reactivity: Human, Mouse, Rat WB, IHC, ELISA Host: Goat Polyclonal unconjugated

anti-Stratifin (SFN) (AA 1-248) antibody
SFN Reactivity: Human WB, ELISA, IHC (p), IP Host: Mouse Monoclonal 3C3 unconjugated

anti-Stratifin (SFN) (C-Term) antibody
SFN Reactivity: Human WB, IF, IHC (p), ICC Host: Rabbit Polyclonal unconjugated

anti-Stratifin (SFN) (AA 140-156) antibody
SFN Reactivity: Human, Mouse, Rat WB, IHC (p), ICC Host: Rabbit Polyclonal unconjugated

anti-Stratifin (SFN) (AA 1-248) antibody
SFN Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Stratifin (SFN) (AA 222-248), (C-Term) antibody
SFN Reactivity: Human WB, IF, IHC (p), FACS Host: Rabbit Polyclonal RB27523 unconjugated

anti-Stratifin (SFN) (AA 1-248) antibody
SFN Reactivity: Human WB, ELISA, IF Host: Mouse Monoclonal 2G2 unconjugated

anti-Stratifin (SFN) antibody
SFN Reactivity: Human WB, IHC, StM Host: Mouse Monoclonal CPTC-SFN-2 unconjugated

anti-Stratifin (SFN) (AA 1-249) antibody
SFN Reactivity: Human WB, IHC, IHC (p) Host: Mouse Monoclonal 3C3 unconjugated

anti-Stratifin (SFN) (AA 140-156), (Middle Region) antibody
SFN Reactivity: Human WB, IHC (p), ICC Host: Rabbit Polyclonal unconjugated

Application Notes

WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment

Positive Control: A549 , 293T , A431 , HeLa , HepG2 , A375 , Neuro2A , GL261 , C8D30
Validation: Orthogonal

Restrictions

For Research Use only
Format

Liquid

Concentration

0.32 mg/mL

Buffer

0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative

Thimerosal (Merthiolate)

Precaution of Use

This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Kim, Morales, Baek, Slaga, DiGiovanni, Kim: "UVB-induced nuclear translocation of TC-PTP by AKT/14-3-3σ axis inhibits keratinocyte survival and proliferation." in: Oncotarget, Vol. 8, Issue 53, pp. 90674-90692, (2017) (PubMed).
Target

14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Alternative Name

stratifin (SFN Products)

Synonyms

YWHAS antibody, Er antibody, Mme1 antibody, Ywhas antibody, stratifin antibody, SFN antibody, Sfn antibody

Background

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. p53-regulated inhibitor of G2/M progression.

Cellular Localization: Cytoplasm , Nucleus , Secreted

Molecular Weight

28 kDa

Gene ID

2810

UniProt

P31947

Pathways

p53 Signaling, Myometrial Relaxation and Contraction