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MMP14 antibody (AA 319-333)

MMP14 Reactivity: Human, Mouse, Rat, Cow, Monkey, Sheep, Goat, Hamster WB, ELISA, IHC, IP, IHC (p), IHC (fro) Host: Mouse Monoclonal unconjugated

Catalog No. ABIN209030

Datasheet as PDF

Target See all MMP14 Antibodies

MMP14 (Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14))
Binding Specificity
14

13

9

8

5

4

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 319-333
Reactivity
94

33

32

2

2

2

1

1

1
Human, Mouse, Rat, Cow, Monkey, Sheep, Goat, Hamster
Host
84

10

1
Mouse
Compatible Secondaries
Clonality
75

20
Monoclonal
Conjugate
51

8

6

6

4

4

2

2

2

2

2

2

2

2
This MMP14 antibody is un-conjugated
Application
64

46

35

18

13

10

9

6

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity

Recognizes human MT1-MMP. Very slight cross-reactivity to hMMP-3. Species cross-reactivity: Mouse, rat, rabbit porcine and equine. For unknown reasons this antibody does not react on westerns to MMP-14 [MT1-MMP], Human, Prodomain, Catalytic Domain.

Predicted Reactivity

Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Goat, Hamster, Porcine, Bovine, Opossum, Platypus (100%) Elephant, Panda, Stickleback, Turkey, Bat, Dog, Horse, Rabbit, Chicken, Pufferfish (93%) Zebrafish, Xenopus, Medaka (87%) Lizard (80%).

Purification

Immunoaffinity purified

Immunogen

Peptide (aa 319-333, CDGNFDTVAMLRGEM) in the hemopexin-like domain of human membrane Type1 matrix metalloproteinase human MT1-MMP also referred to as MMP-14. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Goat, Hamster, Porcine, Bovine, Opossum, Platypus (100%), Elephant, Panda, Stickleback, Turkey, Bat, Dog, Horse, Rabbit, Chicken, Pufferfish (93%), Zebrafish, Xenopus, Medaka (87%), Lizard (80%).

Isotype

IgG3 kappa

Top Product

Discover our top product MMP14 Primary Antibody
anti-Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14) (AA 121-487) antibody
MMP14 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14) (AA 112-246) antibody
MMP14 Reactivity: Human WB, ELISA, IHC, FACS, ICC Host: Mouse Monoclonal 6A11D1 unconjugated

anti-Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14) (C-Term) antibody
MMP14 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, ICC, IF Host: Rabbit Polyclonal unconjugated

anti-Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14) (AA 145-174), (N-Term) antibody
MMP14 Reactivity: Human, Mouse, Rat WB, IHC (p), FACS Host: Rabbit Polyclonal RB02027 unconjugated

anti-Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14) (AA 470-499) antibody
MMP14 Reactivity: Human WB, ELISA, IF Host: Mouse Monoclonal 133CT15-10-5-1 unconjugated

anti-Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14) (AA 322-541) antibody
MMP14 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14) (N-Term) antibody
MMP14 Reactivity: Human WB, ELISA, IHC (p), FACS Host: Rabbit Polyclonal unconjugated

anti-Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14) (Internal Region) antibody
Verified MMP14 Reactivity: Human, Mouse WB, ELISA, IHC Host: Goat Polyclonal unconjugated

anti-Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14) (AA 160-173) antibody
MMP14 Reactivity: Human IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14) (AA 112-321) antibody
MMP14 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

Application Notes

Approved: ELISA, IHC, IHC-Fr, IHC-P, IP, WB (10 μg/mL)

Usage: Suitable for use in ELISA, Western Blot and Immunohistochemistry, and Immunoprecipitation. Western Blot: 10 μg/mL, membrane preparations, MD-MB-231 cells pretreated with 1-5 μg/mL ConA for 24-48 hours for positive control. 60-65kD proteins are identified. When tested against recombinant MT1-MMP (lacking the TM domain) expressed in CHO cells the antibody reacts with a band of ~59kD. Immunohistochemistry: Frozen sections, traditional formalin fixation problematic. Paraffin-embedded tissue sections with PLP fixation required.

Comment

Target Species of Antibody: Human

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

PBS,  pH 7.0, 2 % BSA (protease free).

Storage

4 °C,-20 °C

Storage Comment

Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Target

MMP14 (Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14))

Alternative Name

MMP14 / MMP-14 (MMP14 Products)

Synonyms

MMP1 antibody, MMP-14 antibody, MMP-X1 antibody, MT-MMP antibody, MT-MMP 1 antibody, MT1-MMP antibody, MT1MMP antibody, MTMMP1 antibody, mmp-x1 antibody, mt1-mmp antibody, mtmmp1 antibody, AI325305 antibody, MT-MMP-1 antibody, sabe antibody, Mt1-mmp antibody, MT-MMPbeta antibody, cb386 antibody, sb:cb386 antibody, wu:fb72b09 antibody, mmp14 antibody, matrix metallopeptidase 14 antibody, matrix metallopeptidase 14 (membrane-inserted) antibody, matrix metallopeptidase 14b (membrane-inserted) antibody, matrix metallopeptidase 14a (membrane-inserted) antibody, matrix metallopeptidase 14 L homeolog antibody, MMP14 antibody, mmp14 antibody, Mmp14 antibody, mmp14b antibody, mmp14a antibody, mmp14.L antibody

Background

Name/Gene ID: MMP14
Subfamily: Metallopeptidase M10A
Family: Protease

Synonyms: MMP14, Membrane type-1 MMP, MT1-MMP, MT1MMP, MT-MMP, MT-MMP 1, Matrix metalloproteinase-14, MMP-14, MMP-X1, MTMMP1

Gene ID

4323

UniProt

P50281

Pathways

Autophagy