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MYLK antibody (AA 1441-1709)

Name: MYLK antibody (AA 1441-1709)
SKU: ABIN5693029
Price: 370 USD
Availability: InStock

MYLK Reactivity: Human, Mouse, Rat WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

Western blot analysis of MYLK using anti-MYLK antibody .

IHC analysis of MYLK using anti-MYLK antibody .

IHC

7 images

Catalog No. ABIN5693029

Datasheet as PDF

Target See all MYLK Antibodies

MYLK (Myosin Light Chain Kinase (MYLK))
Binding Specificity
15

8

8

7

5

4

3

3

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 1441-1709
Reactivity
79

41

34

2

2

1

1
Human, Mouse, Rat
Host
87

4

3

1
Rabbit
Compatible Secondaries
Clonality
82

13
Polyclonal
Conjugate
42

8

5

5

5

5

5

5

5

3

1

1

1

1

1

1

1
This MYLK antibody is un-conjugated
Application
39

35

24

13

13

12

5

5

4

4

3

2

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Brand

Picoband™

Cross-Reactivity (Details)

No cross reactivity with other proteins.

Characteristics

Rabbit IgG polyclonal antibody for MYLK detection. Tested with WB, IHC-P, Direct ELISA in Human,Mouse,Rat.

Immunogen

E. coli-derived human MYLK recombinant protein (Position: D1441-D1709).

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MYLK Reactivity: Mouse WB, IHC, ICC, IP Host: Rabbit Polyclonal unconjugated

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MYLK Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated

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MYLK Reactivity: Human, Mouse WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-Myosin Light Chain Kinase (MYLK) (AA 1661-1877) antibody
MYLK Reactivity: Human WB, ELISA, IHC (p), ICC, IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Myosin Light Chain Kinase (MYLK) (C-Term) antibody
MYLK Reactivity: Human, Mouse, Rat WB, ELISA Host: Rabbit Polyclonal unconjugated

anti-Myosin Light Chain Kinase (MYLK) (AA 1-992) antibody
MYLK Reactivity: Human WB, PLA Host: Rabbit Polyclonal unconjugated

anti-Myosin Light Chain Kinase (MYLK) antibody
MYLK Reactivity: Human, Mouse, Rat IHC Host: Rabbit Polyclonal unconjugated

anti-Myosin Light Chain Kinase (MYLK) (AA 908-938) antibody
MYLK Reactivity: Human, Mouse WB, IHC (p) Host: Rabbit Polyclonal unconjugated

Application Notes

Recommended Detection Systems: Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Application Details: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 μg/mL
Direct ELISA, 0.1-0.5 μg/mL

Restrictions

For Research Use only
Format

Lyophilized

Reconstitution

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Target

MYLK (Myosin Light Chain Kinase (MYLK))

Alternative Name

MYLK (MYLK Products)

Synonyms

AAT7 antibody, KRP antibody, MLCK antibody, MLCK1 antibody, MLCK108 antibody, MLCK210 antibody, MSTP083 antibody, MYLK1 antibody, smMLCK antibody, 9530072E15Rik antibody, A930019C19Rik antibody, AW489456 antibody, Mlck antibody, SMMLCK antibody, mlck antibody, fj60a06 antibody, mlck1a antibody, mylk antibody, wu:fj60a06 antibody, zmlck1 antibody, myosin light chain kinase antibody, myosin, light polypeptide kinase antibody, myosin, light chain kinase a antibody, MYLK antibody, Mylk antibody, mylka antibody

Background

Synonyms: Myosin light chain kinase, smooth muscle, MLCK, s  mMLCK, Kinase-related protein, KRP, Telokin, Myosin light chain kinase, smooth muscle, deglutamylated form, MYLK, MLCK, MLCK1, MYLK1
Tissue Specificity: Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. Telokin has been found in a wide variety of adult and fetal tissues. Accumulates in well differentiated enterocytes of the intestinal epithelium in response to tumor necrosis factor (TNF).
Background: Myosin light chain kinase, smooth muscle also known as kinase-related protein (KRP) or telokin is an enzyme that in humans is encoded by the MYLK gene. This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin.

UniProt

Q15746